Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries

Boscalid is an agrochemical recently developed for crop protection and the most significant member of the succinate dehydrogenase inhibitor group of fungicides. In this study, a collection of high-affinity monoclonal antibodies was generated to boscalid. By using a series of haptens with a linker at...

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Autores: Esteve Turrillas, Francesc A., Mercader Badia, Josep Vicent, Agulló, Consuelo, Abad Somovilla, Antonio, Abad Fuentes, Antonio
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/179467
Acceso en línea:http://hdl.handle.net/10261/179467
Access Level:acceso abierto
Palabra clave:Competitive ELISA
Monoclonal antibodies
Fungicide
Strawberries
Boscalid
Metabolite M510F01
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spelling Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberriesEsteve Turrillas, Francesc A.Mercader Badia, Josep VicentAgulló, ConsueloAbad Somovilla, AntonioAbad Fuentes, AntonioCompetitive ELISAMonoclonal antibodiesFungicideStrawberriesBoscalidMetabolite M510F01Boscalid is an agrochemical recently developed for crop protection and the most significant member of the succinate dehydrogenase inhibitor group of fungicides. In this study, a collection of high-affinity monoclonal antibodies was generated to boscalid. By using a series of haptens with a linker at alternative tethering sites of the boscalid framework, specific antibodies were isolated as well as antibodies that also recognized the main boscalid metabolite. Two immunoassays were developed using different ELISA formats. Optimized assays displayed very high sensitivities (limits of detection were near 0.01 µg/L). Trueness and precision for the determination of the target analyte in strawberry samples was evaluated. Moreover, immunoassay performance was validated with a reference chromatographic method using QuEChERS extracts of fruits from fungicide-treated crops. A monitoring study with strawberry samples from local markets was carried out by immunoassay, showing an occurrence of boscalid of 15% with a maximum residue concentration of 43 µg/kg.This work was supported by the Spanish Ministerio de Ciencia e Innovación (AGL2009-12940-C02-01/02/ALI and AGL2012-39965-C02-01/02/ALI) and cofinanced by European Regional Development Funds.Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/179467reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.foodchem.2017.06.013Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1794672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
title Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
spellingShingle Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
Esteve Turrillas, Francesc A.
Competitive ELISA
Monoclonal antibodies
Fungicide
Strawberries
Boscalid
Metabolite M510F01
title_short Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
title_full Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
title_fullStr Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
title_full_unstemmed Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
title_sort Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
dc.creator.none.fl_str_mv Esteve Turrillas, Francesc A.
Mercader Badia, Josep Vicent
Agulló, Consuelo
Abad Somovilla, Antonio
Abad Fuentes, Antonio
author Esteve Turrillas, Francesc A.
author_facet Esteve Turrillas, Francesc A.
Mercader Badia, Josep Vicent
Agulló, Consuelo
Abad Somovilla, Antonio
Abad Fuentes, Antonio
author_role author
author2 Mercader Badia, Josep Vicent
Agulló, Consuelo
Abad Somovilla, Antonio
Abad Fuentes, Antonio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Competitive ELISA
Monoclonal antibodies
Fungicide
Strawberries
Boscalid
Metabolite M510F01
topic Competitive ELISA
Monoclonal antibodies
Fungicide
Strawberries
Boscalid
Metabolite M510F01
description Boscalid is an agrochemical recently developed for crop protection and the most significant member of the succinate dehydrogenase inhibitor group of fungicides. In this study, a collection of high-affinity monoclonal antibodies was generated to boscalid. By using a series of haptens with a linker at alternative tethering sites of the boscalid framework, specific antibodies were isolated as well as antibodies that also recognized the main boscalid metabolite. Two immunoassays were developed using different ELISA formats. Optimized assays displayed very high sensitivities (limits of detection were near 0.01 µg/L). Trueness and precision for the determination of the target analyte in strawberry samples was evaluated. Moreover, immunoassay performance was validated with a reference chromatographic method using QuEChERS extracts of fruits from fungicide-treated crops. A monitoring study with strawberry samples from local markets was carried out by immunoassay, showing an occurrence of boscalid of 15% with a maximum residue concentration of 43 µg/kg.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/179467
url http://hdl.handle.net/10261/179467
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.foodchem.2017.06.013
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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