Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells

[EN] The T-2 toxin is a mycotoxin produced by molds belonging to Fusarium. Among the Fusarium mycotoxins, trichothecenes are frequently reported in food and feed, being the T-2 toxin (T-2) the mycotoxin which possesses the highest toxicity. According to EFSA, T-2 is found in various cereal grains us...

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Autores: Taroncher, Mercedes, Rodríguez-Carrasco, Yelko, Ruiz, María-José, Fuentes López, Cristina|||0000-0002-0589-6550
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/205630
Acceso en línea:https://riunet.upv.es/handle/10251/205630
Access Level:acceso abierto
Palabra clave:HepG2 cells
Micronucleus
Comet assay
Mutagenicity
Genotoxicity
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spelling Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cellsTaroncher, MercedesRodríguez-Carrasco, YelkoRuiz, María-JoséFuentes López, Cristina|||0000-0002-0589-6550HepG2 cellsMicronucleusComet assayMutagenicityGenotoxicity[EN] The T-2 toxin is a mycotoxin produced by molds belonging to Fusarium. Among the Fusarium mycotoxins, trichothecenes are frequently reported in food and feed, being the T-2 toxin (T-2) the mycotoxin which possesses the highest toxicity. According to EFSA, T-2 is found in various cereal grains used in food and feed products, mainly in oats, and it has a high environmental impact due to its mechanisms of toxicity. However, recent information on its genotoxic and mutagenic effects is lacking. This work aimed to evaluate the genotoxic and mutagenic potential of T-2 in vitro. For this purpose, HepG2 cells were exposed to 15, 30, and 60 nM T-2 for 24 h, then the DNA damage was evaluated by the micronucleus and the comet assays. In addition, point mutation analysis was performed by the bacterial reverse mutation test using 0.15-60 nM of T-2 concentrations. The results showed chromosomal damage at 60 nM T-2 since significantly more MN appeared at this concentration than in the control samples. Regarding the comet assay, DNA double helix breaks appeared at all concentrations tested and, in a concentration-dependent manner. However, no mutagenic effects were observed at any of the concentrations tested for the Salmonella typhimurium (S. Typhimurium) strains TA98, TA100, TA1535, TA1537, or the Escherichia coli (E. Coli) WP2 strain in the absence or presence of a metabolic activation system. Therefore, these results showed that T-2 mycotoxin produced genotoxic effects by MN and comet assay, while no mutagenicity was observed. However, further research simulating different metabolic activation pathways and the combined exposure of this mycotoxin with other mutagenic chemicals that could be present in the diet is necessary to discard the mutagenic potential of T-2 fully. These results highlight the carcinogenic potential and danger associated with T-2 exposure and should be considered to prevent associated food risks for the human population.This work is part of a research project funded by the Spanish Ministry of Science and Innovation PID2020-115871RB-I00, MCIN/AEI/10.13039/501100011033. Mercedes Taroncher is grateful for the predoctoral grant PRE2021-096941 provided by the Spanish Ministry of Science and Innovation project. Cristina Fuentes was supported by a Margarita Salas Postdoctoral Fellowship from Universitat Politecnica de Valencia funded by Ministerio de Universidades and the European Union-NextGeneration EU.Elsevier Ireland Ltd.Instituto Universitario de Ingeniería de Alimentos (FoodUPV)European CommissionAgencia Estatal de InvestigaciónUNIVERSIDAD POLITECNICA DE VALENCIARepositorio Institucional de la Universitat Politècnica de València Riunet20242024-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/205630reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-115871RB-I00 EXPOSICION COMBINADA A MICOTOXINAS Y PLAGUICIDAS. EVALUACION DEL RIESGO, SEGURIDAD Y ESTRATEGIAS DE MITIGACIONAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 PRE2021-096941open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial (by-nc) http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2056302026-06-13T07:49:27Z
dc.title.none.fl_str_mv Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
title Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
spellingShingle Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
Taroncher, Mercedes
HepG2 cells
Micronucleus
Comet assay
Mutagenicity
Genotoxicity
title_short Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
title_full Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
title_fullStr Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
title_full_unstemmed Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
title_sort Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells
dc.creator.none.fl_str_mv Taroncher, Mercedes
Rodríguez-Carrasco, Yelko
Ruiz, María-José
Fuentes López, Cristina|||0000-0002-0589-6550
author Taroncher, Mercedes
author_facet Taroncher, Mercedes
Rodríguez-Carrasco, Yelko
Ruiz, María-José
Fuentes López, Cristina|||0000-0002-0589-6550
author_role author
author2 Rodríguez-Carrasco, Yelko
Ruiz, María-José
Fuentes López, Cristina|||0000-0002-0589-6550
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario de Ingeniería de Alimentos (FoodUPV)
European Commission
Agencia Estatal de Investigación
UNIVERSIDAD POLITECNICA DE VALENCIA
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv HepG2 cells
Micronucleus
Comet assay
Mutagenicity
Genotoxicity
topic HepG2 cells
Micronucleus
Comet assay
Mutagenicity
Genotoxicity
description [EN] The T-2 toxin is a mycotoxin produced by molds belonging to Fusarium. Among the Fusarium mycotoxins, trichothecenes are frequently reported in food and feed, being the T-2 toxin (T-2) the mycotoxin which possesses the highest toxicity. According to EFSA, T-2 is found in various cereal grains used in food and feed products, mainly in oats, and it has a high environmental impact due to its mechanisms of toxicity. However, recent information on its genotoxic and mutagenic effects is lacking. This work aimed to evaluate the genotoxic and mutagenic potential of T-2 in vitro. For this purpose, HepG2 cells were exposed to 15, 30, and 60 nM T-2 for 24 h, then the DNA damage was evaluated by the micronucleus and the comet assays. In addition, point mutation analysis was performed by the bacterial reverse mutation test using 0.15-60 nM of T-2 concentrations. The results showed chromosomal damage at 60 nM T-2 since significantly more MN appeared at this concentration than in the control samples. Regarding the comet assay, DNA double helix breaks appeared at all concentrations tested and, in a concentration-dependent manner. However, no mutagenic effects were observed at any of the concentrations tested for the Salmonella typhimurium (S. Typhimurium) strains TA98, TA100, TA1535, TA1537, or the Escherichia coli (E. Coli) WP2 strain in the absence or presence of a metabolic activation system. Therefore, these results showed that T-2 mycotoxin produced genotoxic effects by MN and comet assay, while no mutagenicity was observed. However, further research simulating different metabolic activation pathways and the combined exposure of this mycotoxin with other mutagenic chemicals that could be present in the diet is necessary to discard the mutagenic potential of T-2 fully. These results highlight the carcinogenic potential and danger associated with T-2 exposure and should be considered to prevent associated food risks for the human population.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/205630
url https://riunet.upv.es/handle/10251/205630
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-115871RB-I00 EXPOSICION COMBINADA A MICOTOXINAS Y PLAGUICIDAS. EVALUACION DEL RIESGO, SEGURIDAD Y ESTRATEGIAS DE MITIGACION
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 PRE2021-096941
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial (by-nc)
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial (by-nc)
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland Ltd.
publisher.none.fl_str_mv Elsevier Ireland Ltd.
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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