Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica

Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n = 7) was unimmunized and un...

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Autores: Zafra, R., Pérez-Écija, R. A., Buffoni, Leandro, Pacheco Luque, Lourdes Isabel, Martínez Moreno, A., LaCourse, E. J., Perally, S., Brophy, P. M., Pérez, J.
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/3857
Acceso en línea:http://hdl.handle.net/20.500.12466/3857
Access Level:acceso embargado
Palabra clave:Glutathione transferase
Fasciola hepatica
Vaccine
Liver
2401.12 Parasitología Animal
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spelling Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepaticaZafra, R.Pérez-Écija, R. A.Buffoni, LeandroPacheco Luque, Lourdes IsabelMartínez Moreno, A.LaCourse, E. J.Perally, S.Brophy, P. M.Pérez, J.Glutathione transferaseFasciola hepaticaVaccineLiver2401.12 Parasitología AnimalChanges and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n = 7) was unimmunized and uninfected; group 2 (n = 10) was immunized with adjuvant Quil A and infected; group 3 (n = 10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7–9 days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15 weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and γδ T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-γ in the hepatic inflammatory infiltrates was very occasional.20242024-01-2520132013-06-0120132013-06-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/20.500.12466/3857reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengEC FOOD-CT-2005-023025 (DELIVER) Not availableembargoed accesshttp://purl.org/coar/access_right/c_f1cfReserva de todos los derechosCerradoinfo:eu-repo/semantics/embargoedAccessoai:riucv.ucv.es:20.500.12466/38572026-06-19T08:32:07Z
dc.title.none.fl_str_mv Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica
title Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica
spellingShingle Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica
Zafra, R.
Glutathione transferase
Fasciola hepatica
Vaccine
Liver
2401.12 Parasitología Animal
title_short Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica
title_full Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica
title_fullStr Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica
title_full_unstemmed Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica
title_sort Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica
dc.creator.none.fl_str_mv Zafra, R.
Pérez-Écija, R. A.
Buffoni, Leandro
Pacheco Luque, Lourdes Isabel
Martínez Moreno, A.
LaCourse, E. J.
Perally, S.
Brophy, P. M.
Pérez, J.
author Zafra, R.
author_facet Zafra, R.
Pérez-Écija, R. A.
Buffoni, Leandro
Pacheco Luque, Lourdes Isabel
Martínez Moreno, A.
LaCourse, E. J.
Perally, S.
Brophy, P. M.
Pérez, J.
author_role author
author2 Pérez-Écija, R. A.
Buffoni, Leandro
Pacheco Luque, Lourdes Isabel
Martínez Moreno, A.
LaCourse, E. J.
Perally, S.
Brophy, P. M.
Pérez, J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Glutathione transferase
Fasciola hepatica
Vaccine
Liver
2401.12 Parasitología Animal
topic Glutathione transferase
Fasciola hepatica
Vaccine
Liver
2401.12 Parasitología Animal
description Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n = 7) was unimmunized and uninfected; group 2 (n = 10) was immunized with adjuvant Quil A and infected; group 3 (n = 10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7–9 days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15 weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and γδ T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-γ in the hepatic inflammatory infiltrates was very occasional.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-06-01
2013
2013-06-01
2024
2024-01-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12466/3857
url http://hdl.handle.net/20.500.12466/3857
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv EC FOOD-CT-2005-023025 (DELIVER) Not available
dc.rights.none.fl_str_mv embargoed access
http://purl.org/coar/access_right/c_f1cf
Reserva de todos los derechos
Cerrado
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv embargoed access
http://purl.org/coar/access_right/c_f1cf
Reserva de todos los derechos
Cerrado
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
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