Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water

Antibiotics can modify populations of multidrug-resistant microorganism (MDRO) in urban wastewater. Our objectives were to quantify the differences in MDR Gram-negative bacteria between influents and effluents of WWTPs of a Spanish city and to evaluate the influence of human antibiotic prescriptions...

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Autores: Monge-Olivares, Laura, Peñalva, Germán, Pulido, Marina R., Garrudo, Lara, Doval, Miguel Ángel, Ballesta, Sofía, López Cerero, Lorena
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Universidad de Sevilla (US)
Repositório:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/178931
Acesso em linha:https://hdl.handle.net/11441/178931
https://doi.org/10.1016/j.watres.2025.123706
Access Level:Acceso aberto
Palavra-chave:Wastewater
Wastewater treatment plants (WWTP)
Antibiotic resistance
Extended-spectrum beta-lactamase (ESBL)
Carbapenemase
Culture-based approach
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spelling Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated waterMonge-Olivares, LauraPeñalva, GermánPulido, Marina R.Garrudo, LaraDoval, Miguel ÁngelBallesta, SofíaLópez Cerero, LorenaWastewaterWastewater treatment plants (WWTP)Antibiotic resistanceExtended-spectrum beta-lactamase (ESBL)CarbapenemaseCulture-based approachAntibiotics can modify populations of multidrug-resistant microorganism (MDRO) in urban wastewater. Our objectives were to quantify the differences in MDR Gram-negative bacteria between influents and effluents of WWTPs of a Spanish city and to evaluate the influence of human antibiotic prescriptions, as well as the persistence of these bacteria after treatment and their genetic relatedness to clinical isolates. The mean count of ESBL producers and carbapenemase producers were 3.77 and 2.74 log 10 CFU/ml, respectively. The reduction achieved by water treatment of ESBL-producing organisms was 1.4-log (96.11 %), whereas a 1.8-log reduction (98.36 %) was obtained regarding carbapenemase producing organisms. Aeromonas spp. predominated among MDROs and blaKPC-2 was the main carbapenemase detected in the influent wastewater samples. Among Escherichia coli and Klebsiella pneumoniae influent isolates, 44 % and 30 %, respectively, belonged to high-risk clones. Regarding Enterobacteriaceae, 10.6 % matched clinical isolates and one strain from an ongoing hospital outbreak was identified among raw samples. New MDROs and persistence of certain strains were detected in effluent samples. Quinolone and third-generation cephalosporin prescriptions, flow rate and population density were associated with higher OXA-48 producer counts. Despite reductions, additional technologies should be implemented in WWTPs receiving hospital discharges. Given the prevalence of environmental species, culture-based and metagenomic approaches should be combined to distinguish between human and sewage sources for antibiotic resistance monitoring. Overall, this study shows that WWTPs with secondary treatment are effective at removing MDRO, and antibiotic stewardship is a potential strategy to reduce the release of MDROs.ElsevierMicrobiologíaJunta de AndalucíaMicroCarbaFlux2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/178931https://doi.org/10.1016/j.watres.2025.123706reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPY20_01346 2020-CC23140547https://doi.org/10.1016/j.watres.2025.123706info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1789312026-06-17T12:51:07Z
dc.title.none.fl_str_mv Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water
title Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water
spellingShingle Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water
Monge-Olivares, Laura
Wastewater
Wastewater treatment plants (WWTP)
Antibiotic resistance
Extended-spectrum beta-lactamase (ESBL)
Carbapenemase
Culture-based approach
title_short Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water
title_full Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water
title_fullStr Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water
title_full_unstemmed Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water
title_sort Quantitative study of ESBL and carbapenemase producers in wastewater treatment plants in Seville, Spain: a culture-based detection analysis of raw and treated water
dc.creator.none.fl_str_mv Monge-Olivares, Laura
Peñalva, Germán
Pulido, Marina R.
Garrudo, Lara
Doval, Miguel Ángel
Ballesta, Sofía
López Cerero, Lorena
author Monge-Olivares, Laura
author_facet Monge-Olivares, Laura
Peñalva, Germán
Pulido, Marina R.
Garrudo, Lara
Doval, Miguel Ángel
Ballesta, Sofía
López Cerero, Lorena
author_role author
author2 Peñalva, Germán
Pulido, Marina R.
Garrudo, Lara
Doval, Miguel Ángel
Ballesta, Sofía
López Cerero, Lorena
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología
Junta de Andalucía
MicroCarbaFlux
dc.subject.none.fl_str_mv Wastewater
Wastewater treatment plants (WWTP)
Antibiotic resistance
Extended-spectrum beta-lactamase (ESBL)
Carbapenemase
Culture-based approach
topic Wastewater
Wastewater treatment plants (WWTP)
Antibiotic resistance
Extended-spectrum beta-lactamase (ESBL)
Carbapenemase
Culture-based approach
description Antibiotics can modify populations of multidrug-resistant microorganism (MDRO) in urban wastewater. Our objectives were to quantify the differences in MDR Gram-negative bacteria between influents and effluents of WWTPs of a Spanish city and to evaluate the influence of human antibiotic prescriptions, as well as the persistence of these bacteria after treatment and their genetic relatedness to clinical isolates. The mean count of ESBL producers and carbapenemase producers were 3.77 and 2.74 log 10 CFU/ml, respectively. The reduction achieved by water treatment of ESBL-producing organisms was 1.4-log (96.11 %), whereas a 1.8-log reduction (98.36 %) was obtained regarding carbapenemase producing organisms. Aeromonas spp. predominated among MDROs and blaKPC-2 was the main carbapenemase detected in the influent wastewater samples. Among Escherichia coli and Klebsiella pneumoniae influent isolates, 44 % and 30 %, respectively, belonged to high-risk clones. Regarding Enterobacteriaceae, 10.6 % matched clinical isolates and one strain from an ongoing hospital outbreak was identified among raw samples. New MDROs and persistence of certain strains were detected in effluent samples. Quinolone and third-generation cephalosporin prescriptions, flow rate and population density were associated with higher OXA-48 producer counts. Despite reductions, additional technologies should be implemented in WWTPs receiving hospital discharges. Given the prevalence of environmental species, culture-based and metagenomic approaches should be combined to distinguish between human and sewage sources for antibiotic resistance monitoring. Overall, this study shows that WWTPs with secondary treatment are effective at removing MDRO, and antibiotic stewardship is a potential strategy to reduce the release of MDROs.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/178931
https://doi.org/10.1016/j.watres.2025.123706
url https://hdl.handle.net/11441/178931
https://doi.org/10.1016/j.watres.2025.123706
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PY20_01346 2020-
CC23140547
https://doi.org/10.1016/j.watres.2025.123706
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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