Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection

We have previously reported the presence of m6A in the AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two of these putative m6A-sites are in hairpin (hp) structures in the 3'UTR of the viral RNA3. One site (2012AAACU2016) is in the loop of hpB, within the coat protein binding site 1 (CP...

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Autores: Alvarado-Marchena, Luis, Martínez-Pérez, Mireya, Úbeda, Jesús R, Pallás Benet, Vicente, Aparicio, Frederic
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304839
Acceso en línea:http://hdl.handle.net/10261/304839
https://api.elsevier.com/content/abstract/scopus_id/85136626153
Access Level:acceso abierto
Palabra clave:3′UTR
DRACH motif
N6-methyladenosine
RNA covalent modifications
in vivo AMV replication
plant alfamovirus
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repository_id_str
spelling Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral InfectionAlvarado-Marchena, LuisMartínez-Pérez, MireyaÚbeda, Jesús RPallás Benet, VicenteAparicio, Frederic3′UTRDRACH motifN6-methyladenosineRNA covalent modificationsin vivo AMV replicationplant alfamovirusWe have previously reported the presence of m6A in the AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two of these putative m6A-sites are in hairpin (hp) structures in the 3'UTR of the viral RNA3. One site (2012AAACU2016) is in the loop of hpB, within the coat protein binding site 1 (CPB1), while the other (1900UGACC1904) is in the lower stem of hpE, a loop previously associated with AMV negative-strand RNA synthesis. In this work, we have performed in vivo experiments to assess the role of these two regions, containing the putative m6A-sites in the AMV cycle, by introducing compensatory point mutations to interfere with or abolish the m6A-tag of these sites. Our results suggest that the loop of hpB could be involved in viral replication/accumulation. Meanwhile, in the 1900UGACC1904 motif of the hpE, the maintenance of the adenosine residue and the lower stem hpE structure are necessary for in vivo plus-strand accumulation. These results extend our understanding of the requirements for hpE in the AMV infection cycle, indicating that both the residue identity and the base-pairing capacity in this structure are essential for viral accumulation.Peer reviewed0000-0002-1867-45430000-0003-4954-989XConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/304839https://api.elsevier.com/content/abstract/scopus_id/85136626153reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésVirusesSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3048392026-05-22T06:33:51Z
dc.title.none.fl_str_mv Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
title Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
spellingShingle Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
Alvarado-Marchena, Luis
3′UTR
DRACH motif
N6-methyladenosine
RNA covalent modifications
in vivo AMV replication
plant alfamovirus
title_short Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
title_full Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
title_fullStr Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
title_full_unstemmed Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
title_sort Impact of the Potential m6A Modification Sites at the 3'UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection
dc.creator.none.fl_str_mv Alvarado-Marchena, Luis
Martínez-Pérez, Mireya
Úbeda, Jesús R
Pallás Benet, Vicente
Aparicio, Frederic
author Alvarado-Marchena, Luis
author_facet Alvarado-Marchena, Luis
Martínez-Pérez, Mireya
Úbeda, Jesús R
Pallás Benet, Vicente
Aparicio, Frederic
author_role author
author2 Martínez-Pérez, Mireya
Úbeda, Jesús R
Pallás Benet, Vicente
Aparicio, Frederic
author2_role author
author
author
author
dc.contributor.none.fl_str_mv 0000-0002-1867-4543
0000-0003-4954-989X
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 3′UTR
DRACH motif
N6-methyladenosine
RNA covalent modifications
in vivo AMV replication
plant alfamovirus
topic 3′UTR
DRACH motif
N6-methyladenosine
RNA covalent modifications
in vivo AMV replication
plant alfamovirus
description We have previously reported the presence of m6A in the AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two of these putative m6A-sites are in hairpin (hp) structures in the 3'UTR of the viral RNA3. One site (2012AAACU2016) is in the loop of hpB, within the coat protein binding site 1 (CPB1), while the other (1900UGACC1904) is in the lower stem of hpE, a loop previously associated with AMV negative-strand RNA synthesis. In this work, we have performed in vivo experiments to assess the role of these two regions, containing the putative m6A-sites in the AMV cycle, by introducing compensatory point mutations to interfere with or abolish the m6A-tag of these sites. Our results suggest that the loop of hpB could be involved in viral replication/accumulation. Meanwhile, in the 1900UGACC1904 motif of the hpE, the maintenance of the adenosine residue and the lower stem hpE structure are necessary for in vivo plus-strand accumulation. These results extend our understanding of the requirements for hpE in the AMV infection cycle, indicating that both the residue identity and the base-pairing capacity in this structure are essential for viral accumulation.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/304839
https://api.elsevier.com/content/abstract/scopus_id/85136626153
url http://hdl.handle.net/10261/304839
https://api.elsevier.com/content/abstract/scopus_id/85136626153
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Viruses
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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