Effect of several antioxidants on thawed ram spermatozoa submitted to 37ºC up to four hours
Thawed ram spermatozoa were incubated at 37 ◦C in presence of dehydroascorbic acid (DHA), TEMPOL (TPL), N-acetyl-cysteine (NAC) and rutin (RUT), at 0.1 and 1 mM, in order to test their effects on sperm physiology. Cryopreserved spermatozoa from four rams were thawed, pooled, washed and incubated in...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad de Castilla-La Mancha |
| Repositorio: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/7474 |
| Acceso en línea: | http://hdl.handle.net/10578/7474 |
| Access Level: | acceso abierto |
| Palabra clave: | Antioxidantes Estrés oxidativo Ganado ovino Espermatozoides Vitamina C |
| Sumario: | Thawed ram spermatozoa were incubated at 37 ◦C in presence of dehydroascorbic acid (DHA), TEMPOL (TPL), N-acetyl-cysteine (NAC) and rutin (RUT), at 0.1 and 1 mM, in order to test their effects on sperm physiology. Cryopreserved spermatozoa from four rams were thawed, pooled, washed and incubated in TALP-Hepes with 1 mM or 0.1 mM of each antioxidant, performing a replicate with induced oxidative stress e2+/ascorbate). Motility (CASA), viability and mitochondrial membrane potential (flow cytometry) were analyzed at 2 and 4 h. Lipoperoxidation (MDA production), intracellular ROS and DNA status (TUNEL) were analyzed at 4 h. Antioxidants, except DHA 0.1 mM, decreased motility and kinematic parameters, but had little effect on viability or mitochondrial activity. Except 1 mM DHA, the antioxidants reduced ROS at 4 h. Moreover, NAC 1 mM, rutin and TEMPOL reduced ROS and DNA damage in presence of oxidative stress. NAC, rutin 1 mM and TEMPOL reduced lipoperoxidation in presence of oxidative stress. However, DHA did not affected lipoperoxidation. At 1 mM, DHA increased DNA damage in absence of oxidative stress. DHA effects could arise from spermatozoa having a low capacity for reducing it to ascorbic acid, and it may be tested in presence of other antioxidants or reducing power. Future research should focus in testing if the inhibition of motility observed for NAC, rutin and TEMPOL is reversible. These antioxidants might be useful at lower temperatures (refrigerated storage or cryopreservation) when their protective effects could be advantageous |
|---|