Light signals counteract alterations caused by simulated microgravity in proliferating plant cells

18 p.-9 fig.

Detalhes bibliográficos
Autores: Manzano, Aranzazu, Pereda-Loth, Veronica, De Bures, Anne, Sáez-Vásquez, J., Herranz, Raúl, Medina, F. Javier
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/250904
Acesso em linha:http://hdl.handle.net/10261/250904
Access Level:acceso abierto
Palavra-chave:Abiotic stress
Auxin transport
Cell cycle
Graviresponse
Nucleolin
Ribosome biogenesis
Root meristem
Space plant biology
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spelling Light signals counteract alterations caused by simulated microgravity in proliferating plant cellsManzano, AranzazuPereda-Loth, VeronicaDe Bures, AnneSáez-Vásquez, J.Herranz, RaúlMedina, F. JavierAbiotic stressAuxin transportCell cycleGraviresponseNucleolinRibosome biogenesisRoot meristemSpace plant biology18 p.-9 fig.Premise: Light and gravity are fundamental cues for plant development. Our understanding of the effects of light stimuli on plants in space, without gravity, is key to providing conditions for plants to acclimate to the environment. Here we tested the hypothesis that the alterations caused by the absence of gravity in root meristematic cells can be counteracted by light.Methods: Seedlings of wild‐type Arabidopsis thaliana and two mutants of the essential nucleolar protein nucleolin (nuc1, nuc2) were grown in simulated microgravity,either under a white light photoperiod or under continuous darkness. Key variables of cell proliferation (cell cycle regulation), cell growth (ribosome biogenesis),and auxin transport were measured in the root meristem using in situ cellular markers and transcriptomic methods and compared with those of a 1 g control.Results: The incorporation of a photoperiod regime was sufficient to attenuate or suppress the effects caused by gravitational stress at the cellular level in the root meristem. In all cases, values for variables recorded from samples receiving light stimuli in simulated microgravity were closer to values from the controls than values from samples grown in darkness. Differential sensitivities were obtained for the two nucleolin mutants.Conclusions: Light signals may totally or partially replace gravity signals, significantly improving plant growth and development in microgravity. Despite that, molecular alterations are still compatible with the expected acclimation mechanisms, which need to be better understood. The differential sensitivity of nuc1 and nuc2 mutants to gravitational stress points to new strategies to produce more resilient plants to travel with humans in new extraterrestrial endeavors.This work was funded by the Agencia Estatal de Investigación of the Spanish Ministry of Science an Innovation, Grants#ESP2015‐64323‐R and #RTI2018‐099309‐B‐I00 (co‐funded by EU‐ERDF) to F.J.M., and Bonus Recherche from the UPVD to J.S.V. The use of the facilities of microgravity simulation was provided by the ESA‐CORA‐Ground Based Facilities Program, contract Ref. #4000105761 to F.J.M. and R.H. A.M. was recipient of a contract of the Program for Young Researchers Training of the Agencia Estatal de Investigación of the Spanish Ministry of Science an Innovation Ref. #BES‐2013‐063933.Peer reviewedBotanical Society of AmericaAgencia Estatal de Investigación (España)European CommissionManzano, Aranzazu [0000-0002-0150-0803]Pereda-Loth, Veronica [0000-0002-7365-6217]Sáez-Vásquez, J. [0000-0002-2717-7995]Herranz, Raúl [0000-0002-0246-9449]Medina, F. Javier [0000-0002-0866-7710]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/250904reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/ESP2015‐64323‐Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018‐099309‐B‐I00https://doi.org/10.1002/ajb2.1728Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2509042026-05-22T06:33:51Z
dc.title.none.fl_str_mv Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
title Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
spellingShingle Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
Manzano, Aranzazu
Abiotic stress
Auxin transport
Cell cycle
Graviresponse
Nucleolin
Ribosome biogenesis
Root meristem
Space plant biology
title_short Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
title_full Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
title_fullStr Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
title_full_unstemmed Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
title_sort Light signals counteract alterations caused by simulated microgravity in proliferating plant cells
dc.creator.none.fl_str_mv Manzano, Aranzazu
Pereda-Loth, Veronica
De Bures, Anne
Sáez-Vásquez, J.
Herranz, Raúl
Medina, F. Javier
author Manzano, Aranzazu
author_facet Manzano, Aranzazu
Pereda-Loth, Veronica
De Bures, Anne
Sáez-Vásquez, J.
Herranz, Raúl
Medina, F. Javier
author_role author
author2 Pereda-Loth, Veronica
De Bures, Anne
Sáez-Vásquez, J.
Herranz, Raúl
Medina, F. Javier
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
European Commission
Manzano, Aranzazu [0000-0002-0150-0803]
Pereda-Loth, Veronica [0000-0002-7365-6217]
Sáez-Vásquez, J. [0000-0002-2717-7995]
Herranz, Raúl [0000-0002-0246-9449]
Medina, F. Javier [0000-0002-0866-7710]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Abiotic stress
Auxin transport
Cell cycle
Graviresponse
Nucleolin
Ribosome biogenesis
Root meristem
Space plant biology
topic Abiotic stress
Auxin transport
Cell cycle
Graviresponse
Nucleolin
Ribosome biogenesis
Root meristem
Space plant biology
description 18 p.-9 fig.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/250904
url http://hdl.handle.net/10261/250904
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/ESP2015‐64323‐R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018‐099309‐B‐I00
https://doi.org/10.1002/ajb2.1728
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Botanical Society of America
publisher.none.fl_str_mv Botanical Society of America
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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