Cell adhesion on ti surface with controlled roughness

In this report, the in situ interaction between Saos-2 osteoblast cells and a smooth Ti surface was examined over time. The adhesion kinetics and mechanisms of cellular proliferation were monitored by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). The rate of Sao...

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Detalles Bibliográficos
Autores: Burgos-Asperilla, L., García-Alonso, M. C, Escudero, M L., Alonso, C.
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/676452
Acceso en línea:http://hdl.handle.net/10486/676452
https://dx.doi.org/10.3989/revmetalm.044
Access Level:acceso abierto
Palabra clave:Electrochemical impedance spectroscopy
Quartz crystal microbalance
Saos-2 osteoblasts
Titanium
Física
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spelling Cell adhesion on ti surface with controlled roughnessBurgos-Asperilla, L.García-Alonso, M. CEscudero, M L.Alonso, C.Electrochemical impedance spectroscopyQuartz crystal microbalanceSaos-2 osteoblastsTitaniumFísicaIn this report, the in situ interaction between Saos-2 osteoblast cells and a smooth Ti surface was examined over time. The adhesion kinetics and mechanisms of cellular proliferation were monitored by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). The rate of Saos-2 attachment on Ti surfaces, obtained from the measurements performed with the QCM, is a first-order reaction, with k=2.103 min1. The impedance measurements indicate that in the absence of cells, the Ti resistance diminishes over time (7 days), due to the presence of amino acids and proteins from the culture medium that have been a dsorbed, while in the presence of osteoblasts, this decrease is much greater because of the compounds generated by the cells that accelerate the dissolution of TiAdhesión de osteoblastos sobre una superficie de Ti de rugosidad controlada. En este trabajo, se ha estudiado la interacción in situ entre células osteoblásticas Saos-2 y una superficie de Ti de rugosidad controlada a lo largo del tiempo. El estudio de la cinética y los mecanismos de proliferación celular de adhesión se ha realizado a través de la microbalanza de cristal de cuarzo (QCM) y espectroscopía de impedancia electroquímica (EIS). La velocidad de adhesión de los osteoblastos sobre la superficie de Ti obtenida a través de medidas con la QCM, sigue una reacción de primer orden, con k=2×10−3 min−1. Los ensayos de impedancia indican que, en ausencia de las células, la resistencia del Ti disminuye con el tiempo (7 días), debido a la presencia de aminoácidosy proteínas del medio de cultivo que se han adsorbido, mientras que en presencia de células, esta disminución es mucho mayor debido a los productos metabólicos generados por las células que aceleran la disolución del TiThe authors thank the financial support under projects MAT 2008-06719-C03-01, CTQ2008-05775/ BQU and by the Comunidad Autónoma de Madrid (CAM) through AVANSENS S2009/PPQ-1642Centro Nacional de Investigaciones MetalurgicasDepartamento de Química Física AplicadaFacultad de Ciencias20152015-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/676452https://dx.doi.org/10.3989/revmetalm.044reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6764522026-06-23T12:46:27Z
dc.title.none.fl_str_mv Cell adhesion on ti surface with controlled roughness
title Cell adhesion on ti surface with controlled roughness
spellingShingle Cell adhesion on ti surface with controlled roughness
Burgos-Asperilla, L.
Electrochemical impedance spectroscopy
Quartz crystal microbalance
Saos-2 osteoblasts
Titanium
Física
title_short Cell adhesion on ti surface with controlled roughness
title_full Cell adhesion on ti surface with controlled roughness
title_fullStr Cell adhesion on ti surface with controlled roughness
title_full_unstemmed Cell adhesion on ti surface with controlled roughness
title_sort Cell adhesion on ti surface with controlled roughness
dc.creator.none.fl_str_mv Burgos-Asperilla, L.
García-Alonso, M. C
Escudero, M L.
Alonso, C.
author Burgos-Asperilla, L.
author_facet Burgos-Asperilla, L.
García-Alonso, M. C
Escudero, M L.
Alonso, C.
author_role author
author2 García-Alonso, M. C
Escudero, M L.
Alonso, C.
author2_role author
author
author
dc.contributor.none.fl_str_mv Departamento de Química Física Aplicada
Facultad de Ciencias
dc.subject.none.fl_str_mv Electrochemical impedance spectroscopy
Quartz crystal microbalance
Saos-2 osteoblasts
Titanium
Física
topic Electrochemical impedance spectroscopy
Quartz crystal microbalance
Saos-2 osteoblasts
Titanium
Física
description In this report, the in situ interaction between Saos-2 osteoblast cells and a smooth Ti surface was examined over time. The adhesion kinetics and mechanisms of cellular proliferation were monitored by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). The rate of Saos-2 attachment on Ti surfaces, obtained from the measurements performed with the QCM, is a first-order reaction, with k=2.103 min1. The impedance measurements indicate that in the absence of cells, the Ti resistance diminishes over time (7 days), due to the presence of amino acids and proteins from the culture medium that have been a dsorbed, while in the presence of osteoblasts, this decrease is much greater because of the compounds generated by the cells that accelerate the dissolution of Ti
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/676452
https://dx.doi.org/10.3989/revmetalm.044
url http://hdl.handle.net/10486/676452
https://dx.doi.org/10.3989/revmetalm.044
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Centro Nacional de Investigaciones Metalurgicas
publisher.none.fl_str_mv Centro Nacional de Investigaciones Metalurgicas
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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