Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques

The marine microalga Tisochrysis lutea is a potential and sustainable source of bioactive compounds such as carotenoids and omega-3 fatty acids. In the present work, the extraction of fucoxanthin and docosahexaenoic acid (DHA), the most abundant omega-3 fatty acid which constitutes polar lipids part...

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Detalhes bibliográficos
Autores: García-García, Paz, Ospina, Mónica, Señorans Rodríguez, Fco. Javier
Tipo de documento: artigo
Data de publicação:2024
País:España
Recursos:Universidad Autónoma de Madrid
Repositório:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglês
OAI Identifier:oai:repositorio.uam.es:10486/711879
Acesso em linha:http://hdl.handle.net/10486/711879
https://dx.doi.org/10.1007/s10811-024-03233-x
Access Level:Acceso aberto
Palavra-chave:Tisochrysis Lutea
Haptophyta
2-methyloxolane
Polar Lipids
Fucoxanthin
DHA
UAE
Química
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spelling Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniquesGarcía-García, PazOspina, MónicaSeñorans Rodríguez, Fco. JavierTisochrysis LuteaHaptophyta2-methyloxolanePolar LipidsFucoxanthinDHAUAEQuímicaThe marine microalga Tisochrysis lutea is a potential and sustainable source of bioactive compounds such as carotenoids and omega-3 fatty acids. In the present work, the extraction of fucoxanthin and docosahexaenoic acid (DHA), the most abundant omega-3 fatty acid which constitutes polar lipids particularly in the brain, was studied using advanced extraction techniques with green and bio-based solvents compared to traditional extraction techniques with hazardous organic solvents. The experimental design to maximize the lipid extraction yield by ultrasound-assisted extraction (UAE) was developed, choosing as experimental factors the percentage of solvent (0, 50 and 100% of 2-methyl-tetrahydrofuran or 2-methyloxolane (2-me-THF) in ethanol), the extraction time (20, 30 and 40 min) and temperature (40, 50 and 60 ºC). The highest lipid extraction yields were obtained using ethanol as solvent. Nevertheless, the most interesting extracts based on their chemical composition were obtained when the presence of 2-me-THF in the extraction mixture was greater than that of ethanol. Through analytical techniques such as HPLC-ELSD/DAD and GC-MS as well as spectrophotometric techniques, the contents of polar lipids, fatty acids, total carotenoids and fucoxanthin were quantifed. In addition, the antioxidant capacity of diferent selected extracts was studied, being once again the most interesting those extracted with diferent amounts of 2-me-THF due to its selectivity and enriched composition in high-added value bioactives, mainly fucoxanthin and DHA. Therefore, it is shown the importance of choosing an advanced extraction technique together with the use of green solvents not only to develop procedures that are in agreement with Green Chemistry but also to preserve its bioactivityThis research was financially supported by the Comunidad de Madrid (Spain) through project ALGATEC-CM (P2018/BAA-4532), co-financed by the European Social Fund, and from the Ministry of Science and Innovation, Spain (Number project No. RTI2018-093583-B-I00)SpringerDepartamento de Química Física AplicadaFacultad de Ciencias20242024-04-05research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/711879https://dx.doi.org/10.1007/s10811-024-03233-xreponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7118792026-06-23T12:46:27Z
dc.title.none.fl_str_mv Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques
title Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques
spellingShingle Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques
García-García, Paz
Tisochrysis Lutea
Haptophyta
2-methyloxolane
Polar Lipids
Fucoxanthin
DHA
UAE
Química
title_short Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques
title_full Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques
title_fullStr Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques
title_full_unstemmed Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques
title_sort Tisochrysis lutea as a source of omega-3 polar lipids and fucoxanthin: Extraction and characterization using green solvents and advanced techniques
dc.creator.none.fl_str_mv García-García, Paz
Ospina, Mónica
Señorans Rodríguez, Fco. Javier
author García-García, Paz
author_facet García-García, Paz
Ospina, Mónica
Señorans Rodríguez, Fco. Javier
author_role author
author2 Ospina, Mónica
Señorans Rodríguez, Fco. Javier
author2_role author
author
dc.contributor.none.fl_str_mv Departamento de Química Física Aplicada
Facultad de Ciencias
dc.subject.none.fl_str_mv Tisochrysis Lutea
Haptophyta
2-methyloxolane
Polar Lipids
Fucoxanthin
DHA
UAE
Química
topic Tisochrysis Lutea
Haptophyta
2-methyloxolane
Polar Lipids
Fucoxanthin
DHA
UAE
Química
description The marine microalga Tisochrysis lutea is a potential and sustainable source of bioactive compounds such as carotenoids and omega-3 fatty acids. In the present work, the extraction of fucoxanthin and docosahexaenoic acid (DHA), the most abundant omega-3 fatty acid which constitutes polar lipids particularly in the brain, was studied using advanced extraction techniques with green and bio-based solvents compared to traditional extraction techniques with hazardous organic solvents. The experimental design to maximize the lipid extraction yield by ultrasound-assisted extraction (UAE) was developed, choosing as experimental factors the percentage of solvent (0, 50 and 100% of 2-methyl-tetrahydrofuran or 2-methyloxolane (2-me-THF) in ethanol), the extraction time (20, 30 and 40 min) and temperature (40, 50 and 60 ºC). The highest lipid extraction yields were obtained using ethanol as solvent. Nevertheless, the most interesting extracts based on their chemical composition were obtained when the presence of 2-me-THF in the extraction mixture was greater than that of ethanol. Through analytical techniques such as HPLC-ELSD/DAD and GC-MS as well as spectrophotometric techniques, the contents of polar lipids, fatty acids, total carotenoids and fucoxanthin were quantifed. In addition, the antioxidant capacity of diferent selected extracts was studied, being once again the most interesting those extracted with diferent amounts of 2-me-THF due to its selectivity and enriched composition in high-added value bioactives, mainly fucoxanthin and DHA. Therefore, it is shown the importance of choosing an advanced extraction technique together with the use of green solvents not only to develop procedures that are in agreement with Green Chemistry but also to preserve its bioactivity
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-04-05
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/711879
https://dx.doi.org/10.1007/s10811-024-03233-x
url http://hdl.handle.net/10486/711879
https://dx.doi.org/10.1007/s10811-024-03233-x
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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