A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice

Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of t...

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Autores: Tang, Xiaoqian, Zhang, Qi|||0000-0002-0052-3552, Pividori, María Isabel|||0000-0002-5266-7873, Zhang, Zhaowei, Marty, Jean-Louis|||0000-0002-1425-9887, Catanante, Gaëlle|||0000-0001-7987-7945
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:256090
Acceso en línea:https://ddd.uab.cat/record/256090
https://dx.doi.org/urn:doi:10.3390/bios12020059
Access Level:acceso abierto
Palabra clave:Patulin
Aptamer
Lateral flow assay
On-site
Apple juice
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spelling A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple JuiceTang, XiaoqianZhang, Qi|||0000-0002-0052-3552Pividori, María Isabel|||0000-0002-5266-7873Zhang, ZhaoweiMarty, Jean-Louis|||0000-0002-1425-9887Catanante, Gaëlle|||0000-0001-7987-7945PatulinAptamerLateral flow assayOn-siteApple juicePatulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B (AFB), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice. 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/256090https://dx.doi.org/urn:doi:10.3390/bios12020059reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-106625RB-I00open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2560902026-06-06T12:50:31Z
dc.title.none.fl_str_mv A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
title A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
spellingShingle A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
Tang, Xiaoqian
Patulin
Aptamer
Lateral flow assay
On-site
Apple juice
title_short A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
title_full A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
title_fullStr A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
title_full_unstemmed A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
title_sort A Sensitive Aptasensor Using Biotin-Streptavidin System for Patulin Detection in Apple Juice
dc.creator.none.fl_str_mv Tang, Xiaoqian
Zhang, Qi|||0000-0002-0052-3552
Pividori, María Isabel|||0000-0002-5266-7873
Zhang, Zhaowei
Marty, Jean-Louis|||0000-0002-1425-9887
Catanante, Gaëlle|||0000-0001-7987-7945
author Tang, Xiaoqian
author_facet Tang, Xiaoqian
Zhang, Qi|||0000-0002-0052-3552
Pividori, María Isabel|||0000-0002-5266-7873
Zhang, Zhaowei
Marty, Jean-Louis|||0000-0002-1425-9887
Catanante, Gaëlle|||0000-0001-7987-7945
author_role author
author2 Zhang, Qi|||0000-0002-0052-3552
Pividori, María Isabel|||0000-0002-5266-7873
Zhang, Zhaowei
Marty, Jean-Louis|||0000-0002-1425-9887
Catanante, Gaëlle|||0000-0001-7987-7945
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Patulin
Aptamer
Lateral flow assay
On-site
Apple juice
topic Patulin
Aptamer
Lateral flow assay
On-site
Apple juice
description Patulin contamination in fruits, vegetables, and their products is considered a serious health risk factor for food safety and human health. Thus, a rapid, simple detection method for patulin is becoming important, which could provide a tool for routine screening and food surveys. The objective of this study was to develop a sensitive aptamer-based lateral flow assay (FLA) using Streptavidin functionalized gold nanoparticles for sensitive patulin detection. An excellent dynamic range for patulin detection was obtained (2.7~139.8 ng/mL in the buffer and 7.07~359.5 ng/mL in the sample) with no affinity for other mycotoxins such as zearalenone (ZEN), ochratoxin A (OTA), aflatoxin B (AFB), citrinin or tenuazonic acid (TEA). The limit of detection was 0.19 ng/mL in the buffer and 0.36 ng/mL in the real sample. The recoveries were 83.3% to 107.1%, with a satisfactory RSD value from 6.5% to 7.5%. Hence the established LFA could be used as a rapid, simple, on-site screening tool for PAT determination in apple juice.
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/256090
https://dx.doi.org/urn:doi:10.3390/bios12020059
url https://ddd.uab.cat/record/256090
https://dx.doi.org/urn:doi:10.3390/bios12020059
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-106625RB-I00
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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