Identification of Tomato microRNAs in Late Response to Trichoderma atroviride

[EN] The tomato (Solanum lycopersicum) is an important crop worldwide and is considered a model plant to study stress responses. Small RNAs (sRNAs), 21–24 nucleotides in length, are recognized as a conserved mechanism for regulating gene expression in eukaryotes. Plant endogenous sRNAs, such as micr...

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Detalhes bibliográficos
Autores: Olmo López, Rocío, Martín Quijada, Narciso, Morán Díez, María Eugenia, Hermosa Prieto, María Rosa, Monte Vázquez, Enrique
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/168915
Acesso em linha:http://hdl.handle.net/10366/168915
Access Level:acceso abierto
Palavra-chave:Tomato
Trichoderma
RNA sequencing
MicroRNA
MiRNA target
Priming
Quantitative PCR
2415.02 Biología Molecular de Plantas
2409.92 Genética Molecular de Plantas
2417.19 Fisiología Vegetal
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repository_id_str
spelling Identification of Tomato microRNAs in Late Response to Trichoderma atrovirideOlmo López, RocíoMartín Quijada, NarcisoMorán Díez, María EugeniaHermosa Prieto, María RosaMonte Vázquez, EnriqueTomatoTrichodermaRNA sequencingMicroRNAMiRNA targetPrimingQuantitative PCR2415.02 Biología Molecular de Plantas2409.92 Genética Molecular de Plantas2417.19 Fisiología Vegetal[EN] The tomato (Solanum lycopersicum) is an important crop worldwide and is considered a model plant to study stress responses. Small RNAs (sRNAs), 21–24 nucleotides in length, are recognized as a conserved mechanism for regulating gene expression in eukaryotes. Plant endogenous sRNAs, such as microRNA (miRNA), have been involved in disease resistance. High-throughput RNA sequencing was used to analyze the miRNA profile of the aerial part of 30-day-old tomato plants after the application of the fungus Trichoderma atroviride to the seeds at the transcriptional memory state. Compared to control plants, ten differentially expressed (DE) miRNAs were identified in those inoculated with Trichoderma, five upregulated and five downregulated, of which seven were known (miR166a, miR398-3p, miR408, miR5300, miR6024, miR6027-5p, and miR9471b-3p), and three were putatively novel (novel miR257, novel miR275, and novel miR1767). miRNA expression levels were assessed using real-time quantitative PCR analysis. A plant sRNA target analysis of the DE miRNAs predicted 945 potential target genes, most of them being downregulated (84%). The analysis of KEGG metabolic pathways showed that most of the targets harbored functions associated with plant–pathogen interaction, membrane trafficking, and protein kinases. Expression changes of tomato miRNAs caused by Trichoderma are linked to plant defense responses and appear to have long-lasting effects.Regional Government of Castile and Leon projects (SA094P20, Escalera de Excelencia CLU-2018-04, and IR2020-1-USAL05) supported by the European Regional Development Fund (FEDER) and the Spanish Government R+D+i projects (PID-2021-126575OB-I00 and TED2021-130934B-I00), funded by Spanish MCIN/AEI/10.13039/501100011033, by “FEDER a way of making Europe” and by “European Union/Next Generation EU/PRTR”, respectively.MDPI202620262024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/168915reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésSA094P20CLU-2018-04IR2020-1-USAL05PID-2021-126575OB-I00TED2021-130934B-I00MCIN/AEI/10.13039/501100011033Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1689152026-06-07T06:28:51Z
dc.title.none.fl_str_mv Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
title Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
spellingShingle Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
Olmo López, Rocío
Tomato
Trichoderma
RNA sequencing
MicroRNA
MiRNA target
Priming
Quantitative PCR
2415.02 Biología Molecular de Plantas
2409.92 Genética Molecular de Plantas
2417.19 Fisiología Vegetal
title_short Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
title_full Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
title_fullStr Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
title_full_unstemmed Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
title_sort Identification of Tomato microRNAs in Late Response to Trichoderma atroviride
dc.creator.none.fl_str_mv Olmo López, Rocío
Martín Quijada, Narciso
Morán Díez, María Eugenia
Hermosa Prieto, María Rosa
Monte Vázquez, Enrique
author Olmo López, Rocío
author_facet Olmo López, Rocío
Martín Quijada, Narciso
Morán Díez, María Eugenia
Hermosa Prieto, María Rosa
Monte Vázquez, Enrique
author_role author
author2 Martín Quijada, Narciso
Morán Díez, María Eugenia
Hermosa Prieto, María Rosa
Monte Vázquez, Enrique
author2_role author
author
author
author
dc.subject.none.fl_str_mv Tomato
Trichoderma
RNA sequencing
MicroRNA
MiRNA target
Priming
Quantitative PCR
2415.02 Biología Molecular de Plantas
2409.92 Genética Molecular de Plantas
2417.19 Fisiología Vegetal
topic Tomato
Trichoderma
RNA sequencing
MicroRNA
MiRNA target
Priming
Quantitative PCR
2415.02 Biología Molecular de Plantas
2409.92 Genética Molecular de Plantas
2417.19 Fisiología Vegetal
description [EN] The tomato (Solanum lycopersicum) is an important crop worldwide and is considered a model plant to study stress responses. Small RNAs (sRNAs), 21–24 nucleotides in length, are recognized as a conserved mechanism for regulating gene expression in eukaryotes. Plant endogenous sRNAs, such as microRNA (miRNA), have been involved in disease resistance. High-throughput RNA sequencing was used to analyze the miRNA profile of the aerial part of 30-day-old tomato plants after the application of the fungus Trichoderma atroviride to the seeds at the transcriptional memory state. Compared to control plants, ten differentially expressed (DE) miRNAs were identified in those inoculated with Trichoderma, five upregulated and five downregulated, of which seven were known (miR166a, miR398-3p, miR408, miR5300, miR6024, miR6027-5p, and miR9471b-3p), and three were putatively novel (novel miR257, novel miR275, and novel miR1767). miRNA expression levels were assessed using real-time quantitative PCR analysis. A plant sRNA target analysis of the DE miRNAs predicted 945 potential target genes, most of them being downregulated (84%). The analysis of KEGG metabolic pathways showed that most of the targets harbored functions associated with plant–pathogen interaction, membrane trafficking, and protein kinases. Expression changes of tomato miRNAs caused by Trichoderma are linked to plant defense responses and appear to have long-lasting effects.
publishDate 2024
dc.date.none.fl_str_mv 2024
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/168915
url http://hdl.handle.net/10366/168915
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv SA094P20
CLU-2018-04
IR2020-1-USAL05
PID-2021-126575OB-I00
TED2021-130934B-I00
MCIN/AEI/10.13039/501100011033
dc.rights.none.fl_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403363893837824
score 15,81155

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