Thrive or survive: prokaryotic life in hypersaline soils

Background Soil services are central to life on the planet, with microorganisms as their main drivers. Thus, the evalu‑ ation of soil quality requires an understanding of the principles and factors governing microbial dynamics within it. High salt content is a constraint for life afecting more than...

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Authors: Vera Gargallo, Blanca, Hernández, Marcela, Dumont, Marc G., Ventosa Ucero, Antonio
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/156959
Online Access:https://hdl.handle.net/11441/156959
https://doi.org/10.1186/s40793-023-00475-z
Access Level:Open access
Keyword:Hypersaline environments
Saline soil
Stable isotope probing
Prokaryotic communities
Amplicon sequencing
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spelling Thrive or survive: prokaryotic life in hypersaline soilsVera Gargallo, BlancaHernández, MarcelaDumont, Marc G.Ventosa Ucero, AntonioHypersaline environmentsSaline soilStable isotope probingProkaryotic communitiesAmplicon sequencingBackground Soil services are central to life on the planet, with microorganisms as their main drivers. Thus, the evalu‑ ation of soil quality requires an understanding of the principles and factors governing microbial dynamics within it. High salt content is a constraint for life afecting more than 900 million hectares of land, a number predicted to rise at an alarming rate due to changing climate. Nevertheless, little is known about how microbial life unfolds in these habitats. In this study, DNA stable-isotope probing (DNA-SIP) with 18O-water was used to determine for the frst time the taxa able to grow in hypersaline soil samples (ECe=97.02 dS/m). We further evaluated the role of light on prokary‑ otes growth in this habitat. Results We detected growth of both archaea and bacteria, with taxon-specifc growth patterns providing insights into the drivers of success in saline soils. Phylotypes related to extreme halophiles, including haloarchaea and Salinibacter, which share an energetically efcient mechanism for salt adaptation (salt-in strategy), dominated the active community. Bacteria related to moderately halophilic and halotolerant taxa, such as Staphylococcus, Aliifodinibius, Bradymonadales or Chitinophagales also grew during the incubations, but they incorporated less heavy isotope. Light did not stimulate prokaryotic photosynthesis but instead restricted the growth of most bacteria and reduced the diversity of archaea that grew. Conclusions The results of this study suggest that life in saline soils is energetically expensive and that soil hetero‑ geneity and traits such as exopolysaccharide production or predation may support growth in hypersaline soils. The contribution of phototrophy to supporting the heterotrophic community in saline soils remains unclear. This study paves the way toward a more comprehensive understanding of the functioning of these environments, which is fundamental to their management. Furthermore, it illustrates the potential of further research in saline soils to deepen our understanding of the efect of salinity on microbial communities.MCIN/AEI/10.13039/501100011033 - PID2020-118136 GB-I00Junta de Andalucía y fondos europeos FEDER P20_01066 y BIO-213BMCMicrobiología y ParasitologíaBIO213: Estudio de Microorganismos HalófilosMinisterio de Ciencia e Innovación (MICIN). EspañaAgencia Estatal de Investigación. EspañaJunta de AndalucíaEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/156959https://doi.org/10.1186/s40793-023-00475-zreponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésEnvironmental Microbiome, 18 (1), 17.PID2020-118136 GB-I00P20_01066BIO-213https://dx.doi.org/10.1186/s40793-023-00475-zinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/1569592026-06-17T12:51:07Z
dc.title.none.fl_str_mv Thrive or survive: prokaryotic life in hypersaline soils
title Thrive or survive: prokaryotic life in hypersaline soils
spellingShingle Thrive or survive: prokaryotic life in hypersaline soils
Vera Gargallo, Blanca
Hypersaline environments
Saline soil
Stable isotope probing
Prokaryotic communities
Amplicon sequencing
title_short Thrive or survive: prokaryotic life in hypersaline soils
title_full Thrive or survive: prokaryotic life in hypersaline soils
title_fullStr Thrive or survive: prokaryotic life in hypersaline soils
title_full_unstemmed Thrive or survive: prokaryotic life in hypersaline soils
title_sort Thrive or survive: prokaryotic life in hypersaline soils
dc.creator.none.fl_str_mv Vera Gargallo, Blanca
Hernández, Marcela
Dumont, Marc G.
Ventosa Ucero, Antonio
author Vera Gargallo, Blanca
author_facet Vera Gargallo, Blanca
Hernández, Marcela
Dumont, Marc G.
Ventosa Ucero, Antonio
author_role author
author2 Hernández, Marcela
Dumont, Marc G.
Ventosa Ucero, Antonio
author2_role author
author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
BIO213: Estudio de Microorganismos Halófilos
Ministerio de Ciencia e Innovación (MICIN). España
Agencia Estatal de Investigación. España
Junta de Andalucía
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
dc.subject.none.fl_str_mv Hypersaline environments
Saline soil
Stable isotope probing
Prokaryotic communities
Amplicon sequencing
topic Hypersaline environments
Saline soil
Stable isotope probing
Prokaryotic communities
Amplicon sequencing
description Background Soil services are central to life on the planet, with microorganisms as their main drivers. Thus, the evalu‑ ation of soil quality requires an understanding of the principles and factors governing microbial dynamics within it. High salt content is a constraint for life afecting more than 900 million hectares of land, a number predicted to rise at an alarming rate due to changing climate. Nevertheless, little is known about how microbial life unfolds in these habitats. In this study, DNA stable-isotope probing (DNA-SIP) with 18O-water was used to determine for the frst time the taxa able to grow in hypersaline soil samples (ECe=97.02 dS/m). We further evaluated the role of light on prokary‑ otes growth in this habitat. Results We detected growth of both archaea and bacteria, with taxon-specifc growth patterns providing insights into the drivers of success in saline soils. Phylotypes related to extreme halophiles, including haloarchaea and Salinibacter, which share an energetically efcient mechanism for salt adaptation (salt-in strategy), dominated the active community. Bacteria related to moderately halophilic and halotolerant taxa, such as Staphylococcus, Aliifodinibius, Bradymonadales or Chitinophagales also grew during the incubations, but they incorporated less heavy isotope. Light did not stimulate prokaryotic photosynthesis but instead restricted the growth of most bacteria and reduced the diversity of archaea that grew. Conclusions The results of this study suggest that life in saline soils is energetically expensive and that soil hetero‑ geneity and traits such as exopolysaccharide production or predation may support growth in hypersaline soils. The contribution of phototrophy to supporting the heterotrophic community in saline soils remains unclear. This study paves the way toward a more comprehensive understanding of the functioning of these environments, which is fundamental to their management. Furthermore, it illustrates the potential of further research in saline soils to deepen our understanding of the efect of salinity on microbial communities.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/156959
https://doi.org/10.1186/s40793-023-00475-z
url https://hdl.handle.net/11441/156959
https://doi.org/10.1186/s40793-023-00475-z
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Environmental Microbiome, 18 (1), 17.
PID2020-118136 GB-I00
P20_01066
BIO-213
https://dx.doi.org/10.1186/s40793-023-00475-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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