Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme

Incorporation of 32P into yeast fructose-1,6-bisphosphatase (EC 3.1.3.11) was observed after addition of glucose to a cell suspension incubated with (32P)orthophosphoric acid. The 32P counts were coincident with the enzyme band when immunoprecipitates were subjected to sodium dodecyl sulfate disc ge...

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Detalhes bibliográficos
Autores: Mazón, María J., Gancedo, Juana M., Gancedo, Carlos
Formato: artículo
Estado:Versión publicada
Fecha de publicación:1982
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/259456
Acesso em linha:http://hdl.handle.net/10261/259456
Access Level:acceso abierto
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repository_id_str
spelling Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzymeMazón, María J.Gancedo, Juana M.Gancedo, CarlosIncorporation of 32P into yeast fructose-1,6-bisphosphatase (EC 3.1.3.11) was observed after addition of glucose to a cell suspension incubated with (32P)orthophosphoric acid. The 32P counts were coincident with the enzyme band when immunoprecipitates were subjected to sodium dodecyl sulfate disc gel electrophoresis. The incorporation of phosphate was associated with a decrease in enzyme activity. Approximately 1 mol of phosphate was incorporated/mol of enzyme. The phosphate is bound to the enzyme in a phosphoester linkage with a serine residue. Release of 32P accompanying enzyme reactivation was observed both in vivo and in cell-free extracts.Peer reviewedAmerican Society for Biochemistry and Molecular BiologyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220221982info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/259456reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/S0021-9258(19)68160-3Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2594562026-05-22T06:33:51Z
dc.title.none.fl_str_mv Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme
title Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme
spellingShingle Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme
Mazón, María J.
title_short Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme
title_full Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme
title_fullStr Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme
title_full_unstemmed Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme
title_sort Inactivation of yeast fructose-1,6-bisphosphatase. In vivo phosphorylation of the enzyme
dc.creator.none.fl_str_mv Mazón, María J.
Gancedo, Juana M.
Gancedo, Carlos
author Mazón, María J.
author_facet Mazón, María J.
Gancedo, Juana M.
Gancedo, Carlos
author_role author
author2 Gancedo, Juana M.
Gancedo, Carlos
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Incorporation of 32P into yeast fructose-1,6-bisphosphatase (EC 3.1.3.11) was observed after addition of glucose to a cell suspension incubated with (32P)orthophosphoric acid. The 32P counts were coincident with the enzyme band when immunoprecipitates were subjected to sodium dodecyl sulfate disc gel electrophoresis. The incorporation of phosphate was associated with a decrease in enzyme activity. Approximately 1 mol of phosphate was incorporated/mol of enzyme. The phosphate is bound to the enzyme in a phosphoester linkage with a serine residue. Release of 32P accompanying enzyme reactivation was observed both in vivo and in cell-free extracts.
publishDate 1982
dc.date.none.fl_str_mv 1982
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/259456
url http://hdl.handle.net/10261/259456
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/S0021-9258(19)68160-3
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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