Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits

[EN] A total of 598 F-2 does from a cross between the high and low lines selected divergently for uterine capacity during 10 generations were used in a candidate gene analysis. The presence of major genes affecting the number of implanted embryos and uterine capacity has been suggested in lines dive...

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Autores: Peiró Barber, Rosa Mª|||0000-0002-3009-2343, Santacreu, María-Antonia|||0000-0002-6847-9551, Blasco Mateu, Agustín|||0000-0003-3558-0810, Merchán, M., Argente, M. J., García, M. L., Folch, J. M.
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/202754
Acceso en línea:https://riunet.upv.es/handle/10251/202754
Access Level:acceso abierto
Palabra clave:Rabbits
F2
Progesterone receptor gene
Litter size
Gene polymorphisms
PRODUCCION ANIMAL
GENETICA
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repository_id_str
spelling Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in RabbitsPeiró Barber, Rosa Mª|||0000-0002-3009-2343Santacreu, María-Antonia|||0000-0002-6847-9551Blasco Mateu, Agustín|||0000-0003-3558-0810Merchán, M.Argente, M. J.García, M. L.Folch, J. M.RabbitsF2Progesterone receptor geneLitter sizeGene polymorphismsPRODUCCION ANIMALGENETICA[EN] A total of 598 F-2 does from a cross between the high and low lines selected divergently for uterine capacity during 10 generations were used in a candidate gene analysis. The presence of major genes affecting the number of implanted embryos and uterine capacity has been suggested in lines divergently selected for Uterine capacity. Uterine capacity is it main component of litter size. The progesterone receptor gene was tested as a candidate gene to determine whether polymorphisms explain differences in litter size and its components. Fragments of the promoter region and exons 1-8 were amplified and sequenced. One SNP was found in the promoter region, 2464G > A, three SNPs in the 5'-UTR exon 1, and a silence SNP in exon 7. The first four SNPs were segregated in two haplotypes. The allele G found in the promoter region was found in 75% of the high-line parental animals and in 29% of the low-line parental animals. The GG genotype had 0.5 kits and 0.5 implanted embryos more than the AA genotype. At 48 hr of gestation, the difference in early embryo survival and embryonic stage of development. was small. However, at 72 hr of gestation, the GG genotype had 0.36 embryos more than the AA genotype and also had a more advanced embryonic stage of development, showing a lower percentage of compacted morulae and a higher percentage of blastocysts. The difference in litter size between the GG and CA genotypes was similar to the difference found between homozygote genotypes; however, differences in implanted embryos, early embryo survival, and embryo development were not detected between the GG and GA genotypes.The authors are grateful for the excellent assistance provided by R. Muelas and to W. Mekkawi for his software. This study was funded by projects by the Comision Interministerial de Ciencia y Tecnologia AGL2001-3068 and AGL2005-07624. M. Merchan acknowledges a Formacion de Personal Investigador (FPI) grant from the Ministerio de Ciencia y Tecnologia (MCYT) and R. Peiro an FPI grant from Oficinade Ciencia y Tecnologia de la Generalitat Valenciana (OCYT).The Genetics Society of AmericaDepartamento de Ciencia AnimalDepartamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaInstituto Universitario de Ciencia y Tecnología AnimalEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalGeneralitat ValencianaMinisterio de EducaciónMinisterio de Ciencia y TecnologíaRepositorio Institucional de la Universitat Politècnica de València Riunet20082008-11-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/202754reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMINISTERIO DE CIENCIA Y TECNOLOGIA MINISTERIO DE CIENCIA Y TECNOLOGIA AGL2001-3068-C03-01 ESTUDIO GENETICO Y SELECCION DE LOS COMPONENTES DEL TAMAÑO DE CAMADA DEL CONEJOMINISTERIO DE EDUCACION MINISTERIO DE EDUCACION AGL2005-07624-C03-01 MEJORA GENETICA DEL TAMAÑO DE CAMADA EN CONEJOopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2027542026-06-13T07:49:27Z
dc.title.none.fl_str_mv Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
title Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
spellingShingle Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
Peiró Barber, Rosa Mª|||0000-0002-3009-2343
Rabbits
F2
Progesterone receptor gene
Litter size
Gene polymorphisms
PRODUCCION ANIMAL
GENETICA
title_short Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
title_full Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
title_fullStr Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
title_full_unstemmed Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
title_sort Identification of Single-Nucleotide Polymorphism in the Progesterone Receptor Gene and Its Association With Reproductive Traits in Rabbits
dc.creator.none.fl_str_mv Peiró Barber, Rosa Mª|||0000-0002-3009-2343
Santacreu, María-Antonia|||0000-0002-6847-9551
Blasco Mateu, Agustín|||0000-0003-3558-0810
Merchán, M.
Argente, M. J.
García, M. L.
Folch, J. M.
author Peiró Barber, Rosa Mª|||0000-0002-3009-2343
author_facet Peiró Barber, Rosa Mª|||0000-0002-3009-2343
Santacreu, María-Antonia|||0000-0002-6847-9551
Blasco Mateu, Agustín|||0000-0003-3558-0810
Merchán, M.
Argente, M. J.
García, M. L.
Folch, J. M.
author_role author
author2 Santacreu, María-Antonia|||0000-0002-6847-9551
Blasco Mateu, Agustín|||0000-0003-3558-0810
Merchán, M.
Argente, M. J.
García, M. L.
Folch, J. M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Ciencia Animal
Departamento de Biotecnología
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana
Instituto Universitario de Ciencia y Tecnología Animal
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Generalitat Valenciana
Ministerio de Educación
Ministerio de Ciencia y Tecnología
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Rabbits
F2
Progesterone receptor gene
Litter size
Gene polymorphisms
PRODUCCION ANIMAL
GENETICA
topic Rabbits
F2
Progesterone receptor gene
Litter size
Gene polymorphisms
PRODUCCION ANIMAL
GENETICA
description [EN] A total of 598 F-2 does from a cross between the high and low lines selected divergently for uterine capacity during 10 generations were used in a candidate gene analysis. The presence of major genes affecting the number of implanted embryos and uterine capacity has been suggested in lines divergently selected for Uterine capacity. Uterine capacity is it main component of litter size. The progesterone receptor gene was tested as a candidate gene to determine whether polymorphisms explain differences in litter size and its components. Fragments of the promoter region and exons 1-8 were amplified and sequenced. One SNP was found in the promoter region, 2464G > A, three SNPs in the 5'-UTR exon 1, and a silence SNP in exon 7. The first four SNPs were segregated in two haplotypes. The allele G found in the promoter region was found in 75% of the high-line parental animals and in 29% of the low-line parental animals. The GG genotype had 0.5 kits and 0.5 implanted embryos more than the AA genotype. At 48 hr of gestation, the difference in early embryo survival and embryonic stage of development. was small. However, at 72 hr of gestation, the GG genotype had 0.36 embryos more than the AA genotype and also had a more advanced embryonic stage of development, showing a lower percentage of compacted morulae and a higher percentage of blastocysts. The difference in litter size between the GG and CA genotypes was similar to the difference found between homozygote genotypes; however, differences in implanted embryos, early embryo survival, and embryo development were not detected between the GG and GA genotypes.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-11-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/202754
url https://riunet.upv.es/handle/10251/202754
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MINISTERIO DE CIENCIA Y TECNOLOGIA MINISTERIO DE CIENCIA Y TECNOLOGIA AGL2001-3068-C03-01 ESTUDIO GENETICO Y SELECCION DE LOS COMPONENTES DEL TAMAÑO DE CAMADA DEL CONEJO
MINISTERIO DE EDUCACION MINISTERIO DE EDUCACION AGL2005-07624-C03-01 MEJORA GENETICA DEL TAMAÑO DE CAMADA EN CONEJO
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv The Genetics Society of America
publisher.none.fl_str_mv The Genetics Society of America
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403351700996096
score 15,300719

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