Embryonic development of the grass pufferfish (Takifugu niphobles): From egg to larvae

[EN] Tetraodontidae (pufferfish) family members carry the smallest genomes among vertebrates, and these pocket-sized genomes have directly contributed to our understanding of the structure and evolution of higher animals. The grass pufferfish (Takifugu niphobles) could be considered a potential new...

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Detalles Bibliográficos
Autores: Gallego Albiach, Victor|||0000-0002-9733-0625, Asturiano, Juan F.|||0000-0002-6441-5294, Yoshida, Manabu, Kurokawa, D., Fraser, G.J.
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/101714
Acceso en línea:https://riunet.upv.es/handle/10251/101714
Access Level:acceso abierto
Palabra clave:Fugu
Oocyte
Staging
Embryogenesis
Embryo
PRODUCCION ANIMAL
Descripción
Sumario:[EN] Tetraodontidae (pufferfish) family members carry the smallest genomes among vertebrates, and these pocket-sized genomes have directly contributed to our understanding of the structure and evolution of higher animals. The grass pufferfish (Takifugu niphobles) could be considered a potential new model organism for comparative genomics and development due to the potential access to embryos, and availability of sequence data for two similar genomes: that of spotted green pufferfish (Tetraodon nigroviridis) and Fugu (Takifugu rubripes). In this study, we provide the first description of the normal embryonic development of T niphobles, by drawing comparisons with the closely related species cited above. Embryos were obtained by in vitro fertilization of eggs, and subsequent development was monitored at a constant temperature consistent with natural conditions. T. niphobles development was divided into seven periods of embryogenesis: the zygote, cleavage, blastula, gastrula, segmentation, pharyngula, and hatching periods; and stages subdividing these periods are defined based on morphological characteristics. The developmental stage series described in this study aims to provide the utilization of T niphobles as an experimental model organism for comparative developmental studies.