Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation

The development of efficient and inexpensive methodologies for the treatment of all types of wastewater (manure, municipal wastewaters, etc.) is an increasing need. One of the most promising methods is the usage of photobioreactors. Biomass produced, composed by microalgae and bacteria, use the nutr...

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Autor: González Martín, Javier
Tipo de recurso: tesis de maestría
Fecha de publicación:2019
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/38432
Acceso en línea:http://uvadoc.uva.es/handle/10324/38432
Access Level:acceso abierto
Palabra clave:Pig manure
Photobioreactor
Microalgae
Inhibitor
Solubilization
Monosaccharides
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spelling Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediationGonzález Martín, JavierPig manurePhotobioreactorMicroalgaeInhibitorSolubilizationMonosaccharidesThe development of efficient and inexpensive methodologies for the treatment of all types of wastewater (manure, municipal wastewaters, etc.) is an increasing need. One of the most promising methods is the usage of photobioreactors. Biomass produced, composed by microalgae and bacteria, use the nutrients present in all types of wastewater to generate carbohydrates, proteins and lipids. These products can be further processed and revalorized. The use of the different fractions of the biomass, applying the concept of biorefinery, improves the economic and environmental viability of the global process. Certain aspects of the valorization of the carbohydrate fraction of algae and bacteria biomass grown in pig slurry treatment plants in the form of fermentable sugars were studied in this work. Algal biomass samples, obtained from pig manure treatment in a pilot plant, were subjected to several physical and chemical treatments, followed by an enzymatic hydrolysis step. Sugar release entails co-solubilization of other fractions of biomass (proteins and lipids) and generation of degradation byproducts, which can reduce the efficiency and inhibit further processes. The aim of the study is to identify the most efficient method to maximize sugars production while simultaneously minimizing their degradation. The effect of treatments on the generation of degradation byproducts has been investigated. To deepen the understanding of the processes of nutrient degradation and differentiate degradation due to pretreatment and due to biological action, some treatments (acid hydrolysis, alkaline hydrolysis and bead mill grinding) were applied to pure sources of carbohydrates (cellulose), gelatin (proteins) and lipids (vegetable oil). The concentrations of inhibitors obtained were studied by using multivariate statistical analysis (principal component analysis and hierarchical cluster analysis), demonstrating the effect of the different physical, chemical and enzymatic treatments in the type and concentration of degradation products released. Obtained results revealed that even though high byproduct yields, hydrochloric acid treatment is the most suitable method due to the highest monosaccharide recovery. Chemical treatments resulted in higher degradation, while in physical treatments inhibitors were mainly produced during enzymatic hydrolysis as a result of bacteria metabolism.Departamento de Ingeniería Química y Tecnología del Medio AmbienteMáster en Técnicas Avanzadas en Química. Análisis y Control de Calidad QuímicosVega Alegre, María del SolBolado Rodríguez, SilviaUniversidad de Valladolid. Facultad de Ciencias2019info:eu-repo/semantics/masterThesisapplication/pdfhttp://uvadoc.uva.es/handle/10324/38432reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidInglésinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:uvadoc.uva.es:10324/384322026-06-13T12:44:47Z
dc.title.none.fl_str_mv Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation
title Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation
spellingShingle Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation
González Martín, Javier
Pig manure
Photobioreactor
Microalgae
Inhibitor
Solubilization
Monosaccharides
title_short Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation
title_full Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation
title_fullStr Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation
title_full_unstemmed Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation
title_sort Treatment effect on degradation compounds production of microalgal-bacterial biomass grown in photobioreactors for pig manure remediation
dc.creator.none.fl_str_mv González Martín, Javier
author González Martín, Javier
author_facet González Martín, Javier
author_role author
dc.contributor.none.fl_str_mv Vega Alegre, María del Sol
Bolado Rodríguez, Silvia
Universidad de Valladolid. Facultad de Ciencias
dc.subject.none.fl_str_mv Pig manure
Photobioreactor
Microalgae
Inhibitor
Solubilization
Monosaccharides
topic Pig manure
Photobioreactor
Microalgae
Inhibitor
Solubilization
Monosaccharides
description The development of efficient and inexpensive methodologies for the treatment of all types of wastewater (manure, municipal wastewaters, etc.) is an increasing need. One of the most promising methods is the usage of photobioreactors. Biomass produced, composed by microalgae and bacteria, use the nutrients present in all types of wastewater to generate carbohydrates, proteins and lipids. These products can be further processed and revalorized. The use of the different fractions of the biomass, applying the concept of biorefinery, improves the economic and environmental viability of the global process. Certain aspects of the valorization of the carbohydrate fraction of algae and bacteria biomass grown in pig slurry treatment plants in the form of fermentable sugars were studied in this work. Algal biomass samples, obtained from pig manure treatment in a pilot plant, were subjected to several physical and chemical treatments, followed by an enzymatic hydrolysis step. Sugar release entails co-solubilization of other fractions of biomass (proteins and lipids) and generation of degradation byproducts, which can reduce the efficiency and inhibit further processes. The aim of the study is to identify the most efficient method to maximize sugars production while simultaneously minimizing their degradation. The effect of treatments on the generation of degradation byproducts has been investigated. To deepen the understanding of the processes of nutrient degradation and differentiate degradation due to pretreatment and due to biological action, some treatments (acid hydrolysis, alkaline hydrolysis and bead mill grinding) were applied to pure sources of carbohydrates (cellulose), gelatin (proteins) and lipids (vegetable oil). The concentrations of inhibitors obtained were studied by using multivariate statistical analysis (principal component analysis and hierarchical cluster analysis), demonstrating the effect of the different physical, chemical and enzymatic treatments in the type and concentration of degradation products released. Obtained results revealed that even though high byproduct yields, hydrochloric acid treatment is the most suitable method due to the highest monosaccharide recovery. Chemical treatments resulted in higher degradation, while in physical treatments inhibitors were mainly produced during enzymatic hydrolysis as a result of bacteria metabolism.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
dc.identifier.none.fl_str_mv http://uvadoc.uva.es/handle/10324/38432
url http://uvadoc.uva.es/handle/10324/38432
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
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