Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension

Glaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells (RGCs), with intraocular pressure (IOP) being its primary risk factor. Despite controlling IOP, the neurodegenerative process often continues. Therefore, substances with neuroprotective, antioxidant, and anti...

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Autores: Matamoros Felipe, José Antonio, Rubio-Casado, Sara, Fernández Albarral, José, Martínez López, Miguel Ángel, García Martín, Elena Salobrar, Marco López, Eva María, Paleo-García, Víctor, Hoz Montañana, María Rosa De, López Cuenca, Inés, Elvira-Hurtado, Lorena, Sánchez-Puebla Fernández, Lidia, Ramírez Sebastián, José Manuel, Salazar Corral, Juan José, López Gallardo, Meritxell, Ramírez Sebastián, Ana Isabel
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/114901
Acceso en línea:https://hdl.handle.net/20.500.14352/114901
Access Level:acceso abierto
Palabra clave:617.7:616.9
617.7-007.681
577.15.013
547:617.7-08
ganglion cells
coenzyme Q10
Brn3a
melanopsin
neuroprotection
citicoline
experimental glaucoma
visual pathway
retina
Oftalmología
Neurociencias (Medicina)
Química orgánica (Farmacia)
3201.09 Oftalmología
3207.11 Neuropatología
2306 Química Orgánica
id ES_0d5fec21c2a812c8fabda2cc18ff37ee
oai_identifier_str oai:docta.ucm.es:20.500.14352/114901
network_acronym_str ES
network_name_str España
repository_id_str
spelling Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular HypertensionMatamoros Felipe, José AntonioRubio-Casado, SaraFernández Albarral, JoséMartínez López, Miguel ÁngelGarcía Martín, Elena SalobrarMarco López, Eva MaríaPaleo-García, VíctorHoz Montañana, María Rosa DeLópez Cuenca, InésElvira-Hurtado, LorenaSánchez-Puebla Fernández, LidiaRamírez Sebastián, José ManuelSalazar Corral, Juan JoséLópez Gallardo, MeritxellRamírez Sebastián, Ana Isabel617.7:616.9617.7-007.681577.15.013547:617.7-08ganglion cellscoenzyme Q10Brn3amelanopsinneuroprotectionciticolineexperimental glaucomavisual pathwayretinaOftalmologíaNeurociencias (Medicina)Química orgánica (Farmacia)3201.09 Oftalmología3207.11 Neuropatología2306 Química OrgánicaGlaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells (RGCs), with intraocular pressure (IOP) being its primary risk factor. Despite controlling IOP, the neurodegenerative process often continues. Therefore, substances with neuroprotective, antioxidant, and anti-inflammatory properties could protect against RGC death. This study investigated the neuroprotective effects on RGCs and visual pathway neurons of a compound consisting of citicoline and coenzyme Q10 (CoQ10) in a mouse model of unilateral, laser-induced ocular hypertension (OHT). Four groups of mice were used: vehicle group (n = 6), citicoline + CoQ10 group (n = 6), laser–vehicle group (n = 6), and laser–citicoline + CoQ10 group (n = 6). The citicoline + CoQ10 was administered orally once a day starting 15 days before laser treatment, continuing until sacrifice (7 days post-laser). Retinas, the dorsolateral geniculate nucleus (dLGN), the superior colliculus (SC), and the visual cortex (V1) were analyzed. The citicoline + CoQ10 compound used in the laser–citicoline + CoQ10 group demonstrated (1) an ocular hypotensive effect only at 24 h post-laser; (2) prevention of Brn3a+ RGC death in OHT eyes; and (3) no changes in NeuN+ neurons in the dLGN. This study demonstrates that the oral administration of the citicoline + CoQ10 combination may exert a neuroprotective effect against RGC death in an established rodent model of OHT.MDPIUniversidad Complutense de Madrid20242024-12-2420242024-12-24journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/114901reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1149012026-06-02T12:44:21Z
dc.title.none.fl_str_mv Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
title Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
spellingShingle Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
Matamoros Felipe, José Antonio
617.7:616.9
617.7-007.681
577.15.013
547:617.7-08
ganglion cells
coenzyme Q10
Brn3a
melanopsin
neuroprotection
citicoline
experimental glaucoma
visual pathway
retina
Oftalmología
Neurociencias (Medicina)
Química orgánica (Farmacia)
3201.09 Oftalmología
3207.11 Neuropatología
2306 Química Orgánica
title_short Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
title_full Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
title_fullStr Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
title_full_unstemmed Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
title_sort Neuroprotective Effect of the Combination of Citicoline and CoQ10 in a Mouse Model of Ocular Hypertension
dc.creator.none.fl_str_mv Matamoros Felipe, José Antonio
Rubio-Casado, Sara
Fernández Albarral, José
Martínez López, Miguel Ángel
García Martín, Elena Salobrar
Marco López, Eva María
Paleo-García, Víctor
Hoz Montañana, María Rosa De
López Cuenca, Inés
Elvira-Hurtado, Lorena
Sánchez-Puebla Fernández, Lidia
Ramírez Sebastián, José Manuel
Salazar Corral, Juan José
López Gallardo, Meritxell
Ramírez Sebastián, Ana Isabel
author Matamoros Felipe, José Antonio
author_facet Matamoros Felipe, José Antonio
Rubio-Casado, Sara
Fernández Albarral, José
Martínez López, Miguel Ángel
García Martín, Elena Salobrar
Marco López, Eva María
Paleo-García, Víctor
Hoz Montañana, María Rosa De
López Cuenca, Inés
Elvira-Hurtado, Lorena
Sánchez-Puebla Fernández, Lidia
Ramírez Sebastián, José Manuel
Salazar Corral, Juan José
López Gallardo, Meritxell
Ramírez Sebastián, Ana Isabel
author_role author
author2 Rubio-Casado, Sara
Fernández Albarral, José
Martínez López, Miguel Ángel
García Martín, Elena Salobrar
Marco López, Eva María
Paleo-García, Víctor
Hoz Montañana, María Rosa De
López Cuenca, Inés
Elvira-Hurtado, Lorena
Sánchez-Puebla Fernández, Lidia
Ramírez Sebastián, José Manuel
Salazar Corral, Juan José
López Gallardo, Meritxell
Ramírez Sebastián, Ana Isabel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 617.7:616.9
617.7-007.681
577.15.013
547:617.7-08
ganglion cells
coenzyme Q10
Brn3a
melanopsin
neuroprotection
citicoline
experimental glaucoma
visual pathway
retina
Oftalmología
Neurociencias (Medicina)
Química orgánica (Farmacia)
3201.09 Oftalmología
3207.11 Neuropatología
2306 Química Orgánica
topic 617.7:616.9
617.7-007.681
577.15.013
547:617.7-08
ganglion cells
coenzyme Q10
Brn3a
melanopsin
neuroprotection
citicoline
experimental glaucoma
visual pathway
retina
Oftalmología
Neurociencias (Medicina)
Química orgánica (Farmacia)
3201.09 Oftalmología
3207.11 Neuropatología
2306 Química Orgánica
description Glaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells (RGCs), with intraocular pressure (IOP) being its primary risk factor. Despite controlling IOP, the neurodegenerative process often continues. Therefore, substances with neuroprotective, antioxidant, and anti-inflammatory properties could protect against RGC death. This study investigated the neuroprotective effects on RGCs and visual pathway neurons of a compound consisting of citicoline and coenzyme Q10 (CoQ10) in a mouse model of unilateral, laser-induced ocular hypertension (OHT). Four groups of mice were used: vehicle group (n = 6), citicoline + CoQ10 group (n = 6), laser–vehicle group (n = 6), and laser–citicoline + CoQ10 group (n = 6). The citicoline + CoQ10 was administered orally once a day starting 15 days before laser treatment, continuing until sacrifice (7 days post-laser). Retinas, the dorsolateral geniculate nucleus (dLGN), the superior colliculus (SC), and the visual cortex (V1) were analyzed. The citicoline + CoQ10 compound used in the laser–citicoline + CoQ10 group demonstrated (1) an ocular hypotensive effect only at 24 h post-laser; (2) prevention of Brn3a+ RGC death in OHT eyes; and (3) no changes in NeuN+ neurons in the dLGN. This study demonstrates that the oral administration of the citicoline + CoQ10 combination may exert a neuroprotective effect against RGC death in an established rodent model of OHT.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-12-24
2024
2024-12-24
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/114901
url https://hdl.handle.net/20.500.14352/114901
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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