Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids

Pazos-Perez, Nicolas; Pazos, Elena; Catala, Carme; Mir-Simon, Bernat|||0000-0002-7899-9577; Gómez-de Pedro, Sara|||0000-0002-3794-5820; Sagales, Juan; Villanueva, Carlos; Vila Estapé, Jordi|||0000-0002-8025-3926; Soriano Viladomiu, Alex|||0000-0002-9374-0811; García de Abajo, F. Javier; Álvarez Puebla, Ramon A.|||0000-0003-4770-5756

Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids

Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large sample...

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Autores:	Pazos-Perez, Nicolas, Pazos, Elena, Catala, Carme, Mir-Simon, Bernat\|\|\|0000-0002-7899-9577, Gómez-de Pedro, Sara\|\|\|0000-0002-3794-5820, Sagales, Juan, Villanueva, Carlos, Vila Estapé, Jordi\|\|\|0000-0002-8025-3926, Soriano Viladomiu, Alex\|\|\|0000-0002-9374-0811, García de Abajo, F. Javier, Álvarez Puebla, Ramon A.\|\|\|0000-0003-4770-5756
Tipo de recurso:	artículo
Fecha de publicación:	2016
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:254170
Acceso en línea:	https://ddd.uab.cat/record/254170 https://dx.doi.org/urn:doi:10.1038/srep29014
Access Level:	acceso abierto

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spelling	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluidsPazos-Perez, NicolasPazos, ElenaCatala, CarmeMir-Simon, Bernat\|\|\|0000-0002-7899-9577Gómez-de Pedro, Sara\|\|\|0000-0002-3794-5820Sagales, JuanVillanueva, CarlosVila Estapé, Jordi\|\|\|0000-0002-8025-3926Soriano Viladomiu, Alex\|\|\|0000-0002-9374-0811García de Abajo, F. JavierÁlvarez Puebla, Ramon A.\|\|\|0000-0003-4770-5756Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large samples of blood (~1 milliliter) extending over 24-72 hours. This delay seriously compromises the health of patients. Here we demonstrate a fast microorganism optical detection system for the exhaustive identification and quantification of pathogens in volumes of biofluids with clinical relevance (~1 milliliter) in minutes. We drive each type of bacteria to accumulate antibody functionalized SERS-labelled silver nanoparticles. Particle aggregation on the bacteria membranes renders dense arrays of inter-particle gaps in which the Raman signal is exponentially amplified by several orders of magnitude relative to the dispersed particles. This enables a multiplex identification of the microorganisms through the molecule-specific spectral fingerprints.Universitat Autònoma de Barcelona 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/254170https://dx.doi.org/urn:doi:10.1038/srep29014reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 623527Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2014-59808RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 MAT2014-59096Popen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2541702026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
title	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
spellingShingle	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids Pazos-Perez, Nicolas
title_short	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
title_full	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
title_fullStr	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
title_full_unstemmed	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
title_sort	Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids
dc.creator.none.fl_str_mv	Pazos-Perez, Nicolas Pazos, Elena Catala, Carme Mir-Simon, Bernat\|\|\|0000-0002-7899-9577 Gómez-de Pedro, Sara\|\|\|0000-0002-3794-5820 Sagales, Juan Villanueva, Carlos Vila Estapé, Jordi\|\|\|0000-0002-8025-3926 Soriano Viladomiu, Alex\|\|\|0000-0002-9374-0811 García de Abajo, F. Javier Álvarez Puebla, Ramon A.\|\|\|0000-0003-4770-5756
author	Pazos-Perez, Nicolas
author_facet	Pazos-Perez, Nicolas Pazos, Elena Catala, Carme Mir-Simon, Bernat\|\|\|0000-0002-7899-9577 Gómez-de Pedro, Sara\|\|\|0000-0002-3794-5820 Sagales, Juan Villanueva, Carlos Vila Estapé, Jordi\|\|\|0000-0002-8025-3926 Soriano Viladomiu, Alex\|\|\|0000-0002-9374-0811 García de Abajo, F. Javier Álvarez Puebla, Ramon A.\|\|\|0000-0003-4770-5756
author_role	author
author2	Pazos, Elena Catala, Carme Mir-Simon, Bernat\|\|\|0000-0002-7899-9577 Gómez-de Pedro, Sara\|\|\|0000-0002-3794-5820 Sagales, Juan Villanueva, Carlos Vila Estapé, Jordi\|\|\|0000-0002-8025-3926 Soriano Viladomiu, Alex\|\|\|0000-0002-9374-0811 García de Abajo, F. Javier Álvarez Puebla, Ramon A.\|\|\|0000-0003-4770-5756
author2_role	author author author author author author author author author author
dc.contributor.none.fl_str_mv	Universitat Autònoma de Barcelona
description	Efficient treatments in bacterial infections require the fast and accurate recognition of pathogens, with concentrations as low as one per milliliter in the case of septicemia. Detecting and quantifying bacteria in such low concentrations is challenging and typically demands cultures of large samples of blood (~1 milliliter) extending over 24-72 hours. This delay seriously compromises the health of patients. Here we demonstrate a fast microorganism optical detection system for the exhaustive identification and quantification of pathogens in volumes of biofluids with clinical relevance (~1 milliliter) in minutes. We drive each type of bacteria to accumulate antibody functionalized SERS-labelled silver nanoparticles. Particle aggregation on the bacteria membranes renders dense arrays of inter-particle gaps in which the Raman signal is exponentially amplified by several orders of magnitude relative to the dispersed particles. This enables a multiplex identification of the microorganisms through the molecule-specific spectral fingerprints.
publishDate	2016
dc.date.none.fl_str_mv	2 2016-01-01 2016 2016-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/254170 https://dx.doi.org/urn:doi:10.1038/srep29014
url	https://ddd.uab.cat/record/254170 https://dx.doi.org/urn:doi:10.1038/srep29014
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	European Commission https://doi.org/10.13039/501100000780 623527 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2014-59808R Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 MAT2014-59096P
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,301629

Ultrasensitive multiplex optical quantification of bacteria in large samples of biofluids

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