Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins

25 pags., 6 figs., 3 tabs.

Detalles Bibliográficos
Autores: Campanero-Rhodes, María Asunción, Palma, Angelina S., Menéndez, Margarita, Solís, Dolores
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/221232
Acceso en línea:http://hdl.handle.net/10261/221232
Access Level:acceso abierto
Palabra clave:Bacterial glycans
Microarrays
Lectins
Antibodies
Immune system
Vaccine development
Bacterial interactions
id ES_0d1ef077a8001bfb3c34fcbb31e4cbc4
oai_identifier_str oai:digital.csic.es:10261/221232
network_acronym_str ES
network_name_str España
repository_id_str
spelling Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding ProteinsCampanero-Rhodes, María AsunciónPalma, Angelina S.Menéndez, MargaritaSolís, DoloresBacterial glycansMicroarraysLectinsAntibodiesImmune systemVaccine developmentBacterial interactions25 pags., 6 figs., 3 tabs.Bacterial surfaces are decorated with distinct carbohydrate structures that may substantially differ among species and strains. These structures can be recognized by a variety of glycan-binding proteins, playing an important role in the bacteria cross-talk with the host and invading bacteriophages, and also in the formation of bacterial microcolonies and biofilms. In recent years, different microarray approaches for exploring bacterial surface glycans and their recognition by proteins have been developed. A main advantage of the microarray format is the inherent miniaturization of the method, which allows sensitive and high-throughput analyses with very small amounts of sample. Antibody and lectin microarrays have been used for examining bacterial glycosignatures, enabling bacteria identification and differentiation among strains. In addition, microarrays incorporating bacterial carbohydrate structures have served to evaluate their recognition by diverse host/phage/bacterial glycan-binding proteins, such as lectins, effectors of the immune system, or bacterial and phagic cell wall lysins, and to identify antigenic determinants for vaccine development. The list of samples printed in the arrays includes polysaccharides, lipopoly/lipooligosaccharides, (lipo)teichoic acids, and peptidoglycans, as well as sequence-defined oligosaccharide fragments. Moreover, microarrays of cell wall fragments and entire bacterial cells have been developed, which also allow to study bacterial glycosylation patterns. In this review, examples of the different microarray platforms and applications are presented with a view to give the current state-of-the-art and future prospects in this field.This work was financed by the Spanish Ministry of Economy and Competitiveness (Grant BFU2015-70052-R), the Spanish Ministry of Science, Innovation, and Universities, the Spanish State Research Agency, the European Regional Development Fund (RTI2018-099985-B-I00, MCIU/AEI/FEDER, UE), the CIBER of Respiratory Diseases (CIBERES), an initiative from the Spanish Institute of Health Carlos III (ISCIII), the Fundação para a Ciência e a Tecnologia (FCT-MCTES), Portugal (Grants PTDC/QUI-QUI/112537/2009, PTDC/BIA-MIB/31730/2017, and IF/00023/2012), and the Applied Molecular Biosciences Unit (UCIBIO) (Financed by FCT-MCTES, UID/Multi/04378/2013/2019)Frontiers MediaMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Instituto de Salud Carlos IIIFundação de Apoio à Pesquisa do Rio Grande do NorteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_dcae04bcPublisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/221232reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-70052-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099985-B-I00http://dx.doi.org/10.3389/fmicb.2019.02909Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2212322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins
title Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins
spellingShingle Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins
Campanero-Rhodes, María Asunción
Bacterial glycans
Microarrays
Lectins
Antibodies
Immune system
Vaccine development
Bacterial interactions
title_short Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins
title_full Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins
title_fullStr Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins
title_full_unstemmed Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins
title_sort Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins
dc.creator.none.fl_str_mv Campanero-Rhodes, María Asunción
Palma, Angelina S.
Menéndez, Margarita
Solís, Dolores
author Campanero-Rhodes, María Asunción
author_facet Campanero-Rhodes, María Asunción
Palma, Angelina S.
Menéndez, Margarita
Solís, Dolores
author_role author
author2 Palma, Angelina S.
Menéndez, Margarita
Solís, Dolores
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Instituto de Salud Carlos III
Fundação de Apoio à Pesquisa do Rio Grande do Norte
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacterial glycans
Microarrays
Lectins
Antibodies
Immune system
Vaccine development
Bacterial interactions
topic Bacterial glycans
Microarrays
Lectins
Antibodies
Immune system
Vaccine development
Bacterial interactions
description 25 pags., 6 figs., 3 tabs.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_dcae04bc
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/221232
url http://hdl.handle.net/10261/221232
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-70052-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099985-B-I00
http://dx.doi.org/10.3389/fmicb.2019.02909
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403322067189760
score 15,811543

Similares en LA Referencia