Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior

Human aging is accompanied by a relevant increase in age-associated chronic pathologies, including neurodegenerative and metabolic diseases. The appearance and evolution of numerous neurodegenerative diseases is paralleled by the appearance of intracellular and extracellular accumulation of misfolde...

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Authors: Río Fernández, José Antonio del, Ferrer, Isidro (Ferrer Abizanda)
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/176854
Online Access:https://hdl.handle.net/2445/176854
Access Level:Open access
Keyword:Envelliment
Microfluídica
Aging
Microfluidics
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spelling Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behaviorRío Fernández, José Antonio delFerrer, Isidro (Ferrer Abizanda)EnvellimentMicrofluídicaAgingMicrofluidicsHuman aging is accompanied by a relevant increase in age-associated chronic pathologies, including neurodegenerative and metabolic diseases. The appearance and evolution of numerous neurodegenerative diseases is paralleled by the appearance of intracellular and extracellular accumulation of misfolded proteins in affected brains. In addition, recent evidence suggests that most of these amyloid proteins can behave and propagate among neural cells similarly to infective prions. In order to improve understanding of the seeding and spreading processes of these 'prion-like' amyloids, microfluidics and 3D lab-on-chip approaches have been developed as highly valuable tools. These techniques allow us to monitor changes in cellular and molecular processes responsible for amyloid seeding and cell spreading and their parallel effects in neural physiology. Their compatibility with new optical and biochemical techniques and their relative availability have increased interest in them and in their use in numerous laboratories. In addition, recent advances in stem cell research in combination with microfluidic platforms have opened new humanized in vitro models for myriad neurodegenerative diseases affecting different cellular targets of the vascular, muscular, and nervous systems, and glial cells. These new platforms help reduce the use of animal experimentation. They are more reproducible and represent a potential alternative to classical approaches to understanding neurodegeneration. In this review, we summarize recent progress in neurobiological research in 'prion-like' protein using microfluidic and 3D lab-on-chip approaches. These approaches are driven by various fields, including chemistry, biochemistry, and cell biology, and they serve to facilitate the development of more precise human brain models for basic mechanistic studies of cell-to-cell interactions and drug discovery.Frontiers Media2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/176854Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fbioe.2020.570692Frontiers In Bioengineering And Biotechnology, 2020, vol. 8, num. 570692https://doi.org/10.3389/fbioe.2020.570692cc-by (c) Río Fernández, José Antonio del et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1768542026-05-27T06:46:51Z
dc.title.none.fl_str_mv Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior
title Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior
spellingShingle Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior
Río Fernández, José Antonio del
Envelliment
Microfluídica
Aging
Microfluidics
title_short Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior
title_full Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior
title_fullStr Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior
title_full_unstemmed Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior
title_sort Potential of microfluidics and lab-on-chip platforms to improve understanding of 'prion-like' protein assembly and behavior
dc.creator.none.fl_str_mv Río Fernández, José Antonio del
Ferrer, Isidro (Ferrer Abizanda)
author Río Fernández, José Antonio del
author_facet Río Fernández, José Antonio del
Ferrer, Isidro (Ferrer Abizanda)
author_role author
author2 Ferrer, Isidro (Ferrer Abizanda)
author2_role author
dc.subject.none.fl_str_mv Envelliment
Microfluídica
Aging
Microfluidics
topic Envelliment
Microfluídica
Aging
Microfluidics
description Human aging is accompanied by a relevant increase in age-associated chronic pathologies, including neurodegenerative and metabolic diseases. The appearance and evolution of numerous neurodegenerative diseases is paralleled by the appearance of intracellular and extracellular accumulation of misfolded proteins in affected brains. In addition, recent evidence suggests that most of these amyloid proteins can behave and propagate among neural cells similarly to infective prions. In order to improve understanding of the seeding and spreading processes of these 'prion-like' amyloids, microfluidics and 3D lab-on-chip approaches have been developed as highly valuable tools. These techniques allow us to monitor changes in cellular and molecular processes responsible for amyloid seeding and cell spreading and their parallel effects in neural physiology. Their compatibility with new optical and biochemical techniques and their relative availability have increased interest in them and in their use in numerous laboratories. In addition, recent advances in stem cell research in combination with microfluidic platforms have opened new humanized in vitro models for myriad neurodegenerative diseases affecting different cellular targets of the vascular, muscular, and nervous systems, and glial cells. These new platforms help reduce the use of animal experimentation. They are more reproducible and represent a potential alternative to classical approaches to understanding neurodegeneration. In this review, we summarize recent progress in neurobiological research in 'prion-like' protein using microfluidic and 3D lab-on-chip approaches. These approaches are driven by various fields, including chemistry, biochemistry, and cell biology, and they serve to facilitate the development of more precise human brain models for basic mechanistic studies of cell-to-cell interactions and drug discovery.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176854
url https://hdl.handle.net/2445/176854
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fbioe.2020.570692
Frontiers In Bioengineering And Biotechnology, 2020, vol. 8, num. 570692
https://doi.org/10.3389/fbioe.2020.570692
dc.rights.none.fl_str_mv cc-by (c) Río Fernández, José Antonio del et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Río Fernández, José Antonio del et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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