Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering

The creation of skeletal muscle tissue in vitro is a major topic of interest today in the field of biomedical research, due to the lack of treatments for muscle loss due to traumatic accidents or disease. For this reason, the intrinsic properties of nanofibrillar structures to promote cell adhesion,...

Descripción completa

Detalles Bibliográficos
Autores: Pérez-Puyana, Víctor Manuel, Wieringa, Paul, Yuste, Yaiza, Portilla de Juan, Fernando de la, Guerrero Conejo, Antonio Francisco, Romero García, Alberto, Moroni, Lorenzo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/111277
Acceso en línea:https://hdl.handle.net/11441/111277
https://doi.org/10.1002/jbm.a.37156
Access Level:acceso abierto
Palabra clave:Collagen
Electrospinning
PCL
Scaffolds
Skeletal muscle cells
id ES_0cff49bb026ebe8aee5069f723a4eacc
oai_identifier_str oai:idus.us.es:11441/111277
network_acronym_str ES
network_name_str España
repository_id_str
spelling Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineeringPérez-Puyana, Víctor ManuelWieringa, PaulYuste, YaizaPortilla de Juan, Fernando de laGuerrero Conejo, Antonio FranciscoRomero García, AlbertoMoroni, LorenzoCollagenElectrospinningPCLScaffoldsSkeletal muscle cellsThe creation of skeletal muscle tissue in vitro is a major topic of interest today in the field of biomedical research, due to the lack of treatments for muscle loss due to traumatic accidents or disease. For this reason, the intrinsic properties of nanofibrillar structures to promote cell adhesion, proliferation, and cell alignment presents an attractive tool for regenerative medicine to recreate organized tissues such as muscle. Electrospinning is one of the processing techniques often used for the fabrication of these nanofibrous structures and the combination of synthetic and natural polymers is often required to achieve optimal mechanical and physiochemical properties. Here, polycaprolactone (PCL) is selected as a synthetic polymer used for the fabrication of scaffolds, and the effect of protein addition on the final scaffolds' properties is studied. Collagen and gelatin were the proteins selected and two different concentrations were analyzed (2 and 4 wt/vol%). Different PCL/protein systems were prepared, and a structural, mechanical and functional characterization was performed. The influence of fiber alignment on the properties of the final scaffolds was assessed through morphological, mechanical and biological evaluations. A bioreactor was used to promote cell proliferation and differentiation within the scaffolds. The results revealed that protein addition produced a decrease in the fiber size of the membranes, an increase in their hydrophilicity, and a softening of their mechanical properties. The biological study showed the ability of the selected systems to harbor cells, allow their growth and, potentially, develop musculoskeletal tissues.Ministerio de Economía y Competitividad (MINECO/FEDER, EU) from the Spanish Government CTQ2015-71164-PWileyIngeniería QuímicaTEP229: Tecnología y Diseño de Productos Multicomponentes2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/111277https://doi.org/10.1002/jbm.a.37156reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Biomedical Materials Research part A, March, 1-13.https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.37156info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1112772026-06-17T12:51:07Z
dc.title.none.fl_str_mv Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
title Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
spellingShingle Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
Pérez-Puyana, Víctor Manuel
Collagen
Electrospinning
PCL
Scaffolds
Skeletal muscle cells
title_short Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
title_full Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
title_fullStr Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
title_full_unstemmed Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
title_sort Fabrication of hybrid scaffolds obtained from combinations of PCL with gelatin or collagen via electrospinning for skeletal muscle tissue engineering
dc.creator.none.fl_str_mv Pérez-Puyana, Víctor Manuel
Wieringa, Paul
Yuste, Yaiza
Portilla de Juan, Fernando de la
Guerrero Conejo, Antonio Francisco
Romero García, Alberto
Moroni, Lorenzo
author Pérez-Puyana, Víctor Manuel
author_facet Pérez-Puyana, Víctor Manuel
Wieringa, Paul
Yuste, Yaiza
Portilla de Juan, Fernando de la
Guerrero Conejo, Antonio Francisco
Romero García, Alberto
Moroni, Lorenzo
author_role author
author2 Wieringa, Paul
Yuste, Yaiza
Portilla de Juan, Fernando de la
Guerrero Conejo, Antonio Francisco
Romero García, Alberto
Moroni, Lorenzo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ingeniería Química
TEP229: Tecnología y Diseño de Productos Multicomponentes
dc.subject.none.fl_str_mv Collagen
Electrospinning
PCL
Scaffolds
Skeletal muscle cells
topic Collagen
Electrospinning
PCL
Scaffolds
Skeletal muscle cells
description The creation of skeletal muscle tissue in vitro is a major topic of interest today in the field of biomedical research, due to the lack of treatments for muscle loss due to traumatic accidents or disease. For this reason, the intrinsic properties of nanofibrillar structures to promote cell adhesion, proliferation, and cell alignment presents an attractive tool for regenerative medicine to recreate organized tissues such as muscle. Electrospinning is one of the processing techniques often used for the fabrication of these nanofibrous structures and the combination of synthetic and natural polymers is often required to achieve optimal mechanical and physiochemical properties. Here, polycaprolactone (PCL) is selected as a synthetic polymer used for the fabrication of scaffolds, and the effect of protein addition on the final scaffolds' properties is studied. Collagen and gelatin were the proteins selected and two different concentrations were analyzed (2 and 4 wt/vol%). Different PCL/protein systems were prepared, and a structural, mechanical and functional characterization was performed. The influence of fiber alignment on the properties of the final scaffolds was assessed through morphological, mechanical and biological evaluations. A bioreactor was used to promote cell proliferation and differentiation within the scaffolds. The results revealed that protein addition produced a decrease in the fiber size of the membranes, an increase in their hydrophilicity, and a softening of their mechanical properties. The biological study showed the ability of the selected systems to harbor cells, allow their growth and, potentially, develop musculoskeletal tissues.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/111277
https://doi.org/10.1002/jbm.a.37156
url https://hdl.handle.net/11441/111277
https://doi.org/10.1002/jbm.a.37156
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Biomedical Materials Research part A, March, 1-13.
https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.37156
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403315159171072
score 15,300724

Similares en LA Referencia