Impact of sperm DNA damage and oocyte-repairing capacity on trout development

Palabras clave extraídas del título

Detalles Bibliográficos
Autores: Fernández Díez, Cristina, González Rojo, Silvia, Lombó Alonso, Marta, Herráez Ortega, María Paz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Ajuntament de Barcelona
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18707
Acceso en línea:https://rep.bioscientifica.com/view/journals/rep/152/1/57.xml
https://hdl.handle.net/10612/18707
Access Level:acceso abierto
Palabra clave:Biología
Sperm
DNA damage
Oocyte repairing
Trout development
2407 Biología Celular
3104.11 Reproducción
2401.07 Embriología Animal
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repository_id_str
spelling Impact of sperm DNA damage and oocyte-repairing capacity on trout developmentFernández Díez, CristinaGonzález Rojo, SilviaLombó Alonso, MartaHerráez Ortega, María PazBiologíaSpermDNA damageOocyte repairingTrout development2407 Biología Celular3104.11 Reproducción2401.07 Embriología AnimalPalabras clave extraídas del título[EN] Zygotic repair of paternal DNA is essential during embryo development. In spite of the interest devoted to sperm DNA damage, its combined effect with defect-repairing oocytes has not been analyzed. Modification of the breeding season is a common practice in aquaculture. This practice reduces developmental success and could affect the both factors: sperm DNA integrity and oocyte repair capacity. To evaluate the maternal role, we analyzed the progeny outcome after fertilizing in-season trout oocytes with untreated and with UV-irradiated sperm. We also analyzed the offspring obtained out of season with untreated sperm. The analysis of the number of lesions in 4 sperm nuclear genes revealed an increase of 1.22–11.18 lesions/10 kb in out-of-season sperm, similar to that obtained after sperm UV irradiation (400 µW/cm25 min). Gene expression showed in out-of-season oocytes the overexpression of repair genes (ogg1, ung, lig3, rad1) and downregulation of tp53, indicating an enhanced repairing activity and reduced capacity to arrest development upon damage. The analysis of the progeny in out-of-season embryos revealed a similar profile tolerant to DNA damage, leading to a much lower apoptotic activity at organogenesis, lower hatching rates and increased rate of malformations. The effects were milder in descendants from in-season-irradiated sperm, showing an enhanced repairing activity at epibolia. Results point out the importance of the repairing machinery provided by the oocyte and show how susceptible it is to environmental changes. Transcripts related to DNA damage signalization and repair could be used as markers of oocyte qualitySIThis work was supported by the Spanish Ministry of Economy and Competitiveness (project AGL2011-27787)Society for Reproduction and FertilityBiologia CelularFacultad de Ciencias Biologicas y Ambientales2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://rep.bioscientifica.com/view/journals/rep/152/1/57.xmlhttps://hdl.handle.net/10612/18707reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Ajuntament de BarcelonaInglésinfo:eu-repo/grantAgreement/MINECO/ Plan Nacional de I+D+i/ AGL2011-27787info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/187072026-06-24T12:43:27Z
dc.title.none.fl_str_mv Impact of sperm DNA damage and oocyte-repairing capacity on trout development
title Impact of sperm DNA damage and oocyte-repairing capacity on trout development
spellingShingle Impact of sperm DNA damage and oocyte-repairing capacity on trout development
Fernández Díez, Cristina
Biología
Sperm
DNA damage
Oocyte repairing
Trout development
2407 Biología Celular
3104.11 Reproducción
2401.07 Embriología Animal
title_short Impact of sperm DNA damage and oocyte-repairing capacity on trout development
title_full Impact of sperm DNA damage and oocyte-repairing capacity on trout development
title_fullStr Impact of sperm DNA damage and oocyte-repairing capacity on trout development
title_full_unstemmed Impact of sperm DNA damage and oocyte-repairing capacity on trout development
title_sort Impact of sperm DNA damage and oocyte-repairing capacity on trout development
dc.creator.none.fl_str_mv Fernández Díez, Cristina
González Rojo, Silvia
Lombó Alonso, Marta
Herráez Ortega, María Paz
author Fernández Díez, Cristina
author_facet Fernández Díez, Cristina
González Rojo, Silvia
Lombó Alonso, Marta
Herráez Ortega, María Paz
author_role author
author2 González Rojo, Silvia
Lombó Alonso, Marta
Herráez Ortega, María Paz
author2_role author
author
author
dc.contributor.none.fl_str_mv Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Sperm
DNA damage
Oocyte repairing
Trout development
2407 Biología Celular
3104.11 Reproducción
2401.07 Embriología Animal
topic Biología
Sperm
DNA damage
Oocyte repairing
Trout development
2407 Biología Celular
3104.11 Reproducción
2401.07 Embriología Animal
description Palabras clave extraídas del título
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://rep.bioscientifica.com/view/journals/rep/152/1/57.xml
https://hdl.handle.net/10612/18707
url https://rep.bioscientifica.com/view/journals/rep/152/1/57.xml
https://hdl.handle.net/10612/18707
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/ Plan Nacional de I+D+i/ AGL2011-27787
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for Reproduction and Fertility
publisher.none.fl_str_mv Society for Reproduction and Fertility
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Ajuntament de Barcelona
instname_str Ajuntament de Barcelona
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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