T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion

background - Current laboratory tests fail to evaluate the hemostatic function of platelets in patients with thrombocytopenia. We investigated the use of the Total Thrombus-Formation Analysis System (T-TAS® 01 [Fujimori Kogyo Co, Tokyo, Japan]) to evaluate hemostasis under conditions of experimental...

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Autores: Sanz, Cristina, Escolar, Ginès, Diaz-Ricart, Maribel|||0000-0003-1122-0052, Samanbar, Sahar, Piñeyroa, Juan A., Moreno-Castaño, Ana Belén|||0000-0003-0338-7514, Pino, Marc, Torramadé-Moix, Sergi, Martínez Sánchez, Julia, Lozano, Miquel|||0000-0003-2593-833X
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:302112
Acceso en línea:https://ddd.uab.cat/record/302112
https://dx.doi.org/urn:doi:10.2450/BloodTransfus.550
Access Level:acceso abierto
Palabra clave:Thrombocytopenia
Total thrombus-formation analysis system (T-TAS)
Platelet transfusion
Bleeding
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spelling T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusionSanz, CristinaEscolar, GinèsDiaz-Ricart, Maribel|||0000-0003-1122-0052Samanbar, SaharPiñeyroa, Juan A.Moreno-Castaño, Ana Belén|||0000-0003-0338-7514Pino, MarcTorramadé-Moix, SergiMartínez Sánchez, JuliaLozano, Miquel|||0000-0003-2593-833XThrombocytopeniaTotal thrombus-formation analysis system (T-TAS)Platelet transfusionBleedingbackground - Current laboratory tests fail to evaluate the hemostatic function of platelets in patients with thrombocytopenia. We investigated the use of the Total Thrombus-Formation Analysis System (T-TAS® 01 [Fujimori Kogyo Co, Tokyo, Japan]) to evaluate hemostasis under conditions of experimental thrombocytopenia, and in patients before and after platelet transfusion. materials and methods - Specific T-TAS 01 chips, for thrombocytopenic conditions, were used. The area under the curve (AUC) and occlusion time (OT, min) were measured in: (i) experimentally induced thrombocytopenia (183±15 to 6.3±1.2×10 platelets/µL) in blood samples from healthy donors (No.=13), and (ii) blood from oncohematological thrombocytopenic patients (No.=48), before and after platelet transfusion. The influences of hematocrit and number of transfusions were analyzed in these patients. results - Progressive reductions of AUC and prolongations of OT related significantly to decreasing platelet counts (p<0.05 for all) in experimental thrombocytopenia. In samples from thrombocytopenic patients, platelet counts, AUC and OT were, respectively, 10.8±0.6×10/µL, 175.2±59, and 27.2±1 min before transfusion; and 22±1.5×10/µL, 400.8±83 and 22.9±1.5 min after platelet transfusion (p<0.01 for all). A hematocrit below 25% or exposure to ten or more previous platelet transfusions had a negative impact on the T-TAS 01 performance in patients. In vitro correction of the hematocrit improved the hemostatic response in thrombocytopenic patients. discussion - T-TAS 01 measurements were sensitive to low platelet counts in the experimental setting. The technology was sensitive to evaluate the hemostatic capacity of platelet transfusions. Exposure to multiple medications, repeated platelet transfusions and lower hematocrits may interfere with the hemostatic performance in oncohematological patients with thrombocytopenia. 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/302112https://dx.doi.org/urn:doi:10.2450/BloodTransfus.550reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI19/00888"la Caixa" Foundation https://doi.org/10.13039/100010434 202026-10Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-675open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3021122026-06-06T12:50:31Z
dc.title.none.fl_str_mv T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
title T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
spellingShingle T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
Sanz, Cristina
Thrombocytopenia
Total thrombus-formation analysis system (T-TAS)
Platelet transfusion
Bleeding
title_short T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
title_full T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
title_fullStr T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
title_full_unstemmed T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
title_sort T-TAs® 01 as a new tool for the evaluation of hemostasis in thrombocytopenic patients after platelet transfusion
dc.creator.none.fl_str_mv Sanz, Cristina
Escolar, Ginès
Diaz-Ricart, Maribel|||0000-0003-1122-0052
Samanbar, Sahar
Piñeyroa, Juan A.
Moreno-Castaño, Ana Belén|||0000-0003-0338-7514
Pino, Marc
Torramadé-Moix, Sergi
Martínez Sánchez, Julia
Lozano, Miquel|||0000-0003-2593-833X
author Sanz, Cristina
author_facet Sanz, Cristina
Escolar, Ginès
Diaz-Ricart, Maribel|||0000-0003-1122-0052
Samanbar, Sahar
Piñeyroa, Juan A.
Moreno-Castaño, Ana Belén|||0000-0003-0338-7514
Pino, Marc
Torramadé-Moix, Sergi
Martínez Sánchez, Julia
Lozano, Miquel|||0000-0003-2593-833X
author_role author
author2 Escolar, Ginès
Diaz-Ricart, Maribel|||0000-0003-1122-0052
Samanbar, Sahar
Piñeyroa, Juan A.
Moreno-Castaño, Ana Belén|||0000-0003-0338-7514
Pino, Marc
Torramadé-Moix, Sergi
Martínez Sánchez, Julia
Lozano, Miquel|||0000-0003-2593-833X
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Thrombocytopenia
Total thrombus-formation analysis system (T-TAS)
Platelet transfusion
Bleeding
topic Thrombocytopenia
Total thrombus-formation analysis system (T-TAS)
Platelet transfusion
Bleeding
description background - Current laboratory tests fail to evaluate the hemostatic function of platelets in patients with thrombocytopenia. We investigated the use of the Total Thrombus-Formation Analysis System (T-TAS® 01 [Fujimori Kogyo Co, Tokyo, Japan]) to evaluate hemostasis under conditions of experimental thrombocytopenia, and in patients before and after platelet transfusion. materials and methods - Specific T-TAS 01 chips, for thrombocytopenic conditions, were used. The area under the curve (AUC) and occlusion time (OT, min) were measured in: (i) experimentally induced thrombocytopenia (183±15 to 6.3±1.2×10 platelets/µL) in blood samples from healthy donors (No.=13), and (ii) blood from oncohematological thrombocytopenic patients (No.=48), before and after platelet transfusion. The influences of hematocrit and number of transfusions were analyzed in these patients. results - Progressive reductions of AUC and prolongations of OT related significantly to decreasing platelet counts (p<0.05 for all) in experimental thrombocytopenia. In samples from thrombocytopenic patients, platelet counts, AUC and OT were, respectively, 10.8±0.6×10/µL, 175.2±59, and 27.2±1 min before transfusion; and 22±1.5×10/µL, 400.8±83 and 22.9±1.5 min after platelet transfusion (p<0.01 for all). A hematocrit below 25% or exposure to ten or more previous platelet transfusions had a negative impact on the T-TAS 01 performance in patients. In vitro correction of the hematocrit improved the hemostatic response in thrombocytopenic patients. discussion - T-TAS 01 measurements were sensitive to low platelet counts in the experimental setting. The technology was sensitive to evaluate the hemostatic capacity of platelet transfusions. Exposure to multiple medications, repeated platelet transfusions and lower hematocrits may interfere with the hemostatic performance in oncohematological patients with thrombocytopenia.
publishDate 2024
dc.date.none.fl_str_mv 2
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/302112
https://dx.doi.org/urn:doi:10.2450/BloodTransfus.550
url https://ddd.uab.cat/record/302112
https://dx.doi.org/urn:doi:10.2450/BloodTransfus.550
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI19/00888
"la Caixa" Foundation https://doi.org/10.13039/100010434 202026-10
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-675
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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repository.mail.fl_str_mv
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