Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply

46 Pags.- 3 Tabls.- 5 Figs. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

Detalhes bibliográficos
Autores: Ceballos-Laita, Laura, Gutiérrez-Carbonell, Elaín, Lattanzio, Giuseppe, Vázquez-Reina, Saúl, Contreras-Moreira, Bruno, Abadía Bayona, Anunciación, Abadía Bayona, Javier, López-Millán, Ana Flor
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2015
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/111454
Acesso em linha:http://hdl.handle.net/10261/111454
Access Level:Acceso aberto
Palavra-chave:leaf apoplast
iron deficiency
Proteome
Sugar beet
two-dimensional electrophoresis
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spelling Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupplyCeballos-Laita, LauraGutiérrez-Carbonell, ElaínLattanzio, GiuseppeVázquez-Reina, SaúlContreras-Moreira, BrunoAbadía Bayona, AnunciaciónAbadía Bayona, JavierLópez-Millán, Ana Florleaf apoplastiron deficiencyProteomeSugar beettwo-dimensional electrophoresis46 Pags.- 3 Tabls.- 5 Figs. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).The fluid collected by direct leaf centrifugation has been used to study the proteome of the sugar beet apoplastic fluid as well as the changes induced by Fe deficiency and Fe resupply to Fe-deficient plants in the protein profile. Plants were grown in Fe-sufficient and Fe-deficient conditions, and Fe resupply was carried out with 45 μM Fe(III)-EDTA for 24 h. Protein extracts of leaf apoplastic fluid were analyzed by two-dimensional isoelectric focusing-SDS-PAGE electrophoresis. Gel image analysis revealed 203 consistent spots, and proteins in 81% of them (164) were identified by nLC-MS/MS using a custom made reference repository of beet protein sequences. When redundant UniProt entries were deleted, a non-redundant leaf apoplastic proteome consisting of 109 proteins was obtained. TargetP and SecretomeP algorithms predicted that 63% of them were secretory proteins. Functional classification of the non-redundant proteins indicated that stress and defense, protein metabolism, cell wall and C metabolism accounted for approximately 75% of the identified proteome. The effects of Fe-deficiency on the leaf apoplast proteome were limited, with only five spots (2.5%) changing in relative abundance, thus suggesting that protein homeostasis in the leaf apoplast fluid is well maintained upon Fe shortage. The identification of three chitinase isoforms among proteins increasing in relative abundance with Fe-deficiency suggests that one of the few effects of Fe deficiency in the leaf apoplast proteome includes cell wall modifications. Iron resupply to Fe deficient plants changed the relative abundance of 16 spots when compared to either Fe-sufficient or Fe-deficient samples. Proteins identified in these spots can be broadly classified as those responding to Fe-resupply, which included defense and cell wall related proteins, and non-responsive, which are mainly protein metabolism related proteins and whose changes in relative abundance followed the same trend as with Fe-deficiency.Work supported by the Spanish Ministry of Science and Competitiveness (MINECO; projects AGL2012-31988 and AGL2013-42175-R (co-financed with FEDER), the Aragón Government (group A03). L.C.-L., E.G.-C., G.L. and S.V. were supported by FPI-MINECO, I3P-CSIC and FPI-MINECO predoctoral contracts and a JAE-CSIC postdoctoral contract (co-financed by the European Social Fund), respectively.Peer reviewedFrontiers MediaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/111454reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3389/fpls.2015.00145Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1114542026-05-22T06:33:51Z
dc.title.none.fl_str_mv Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply
title Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply
spellingShingle Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply
Ceballos-Laita, Laura
leaf apoplast
iron deficiency
Proteome
Sugar beet
two-dimensional electrophoresis
title_short Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply
title_full Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply
title_fullStr Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply
title_full_unstemmed Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply
title_sort Protein profile of Beta vulgaris leaf apoplastic fluid and changes induced by Fe deficiency and Fe resupply
dc.creator.none.fl_str_mv Ceballos-Laita, Laura
Gutiérrez-Carbonell, Elaín
Lattanzio, Giuseppe
Vázquez-Reina, Saúl
Contreras-Moreira, Bruno
Abadía Bayona, Anunciación
Abadía Bayona, Javier
López-Millán, Ana Flor
author Ceballos-Laita, Laura
author_facet Ceballos-Laita, Laura
Gutiérrez-Carbonell, Elaín
Lattanzio, Giuseppe
Vázquez-Reina, Saúl
Contreras-Moreira, Bruno
Abadía Bayona, Anunciación
Abadía Bayona, Javier
López-Millán, Ana Flor
author_role author
author2 Gutiérrez-Carbonell, Elaín
Lattanzio, Giuseppe
Vázquez-Reina, Saúl
Contreras-Moreira, Bruno
Abadía Bayona, Anunciación
Abadía Bayona, Javier
López-Millán, Ana Flor
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv leaf apoplast
iron deficiency
Proteome
Sugar beet
two-dimensional electrophoresis
topic leaf apoplast
iron deficiency
Proteome
Sugar beet
two-dimensional electrophoresis
description 46 Pags.- 3 Tabls.- 5 Figs. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/111454
url http://hdl.handle.net/10261/111454
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3389/fpls.2015.00145
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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