Novel features of telomere biology revealed by the absence of telomeric DNA methylation

Cytosine methylation regulates the length and stability of telomeres, which can affect a wide variety of biological features, including cell differentiation, development, or illness. Although it is well established that subtelomeric regions are methylated, the presence of methylated cytosines at tel...

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Autores: Vega Vaquero, Alejandro, Bonoro, Giancarlo, Morselli, Marco, Vaquero Sedas, María Isabel, Rubbi, Liudmilla, Pellegrini, M., Vega Palas, Miguel Ángel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/68550
Acceso en línea:http://hdl.handle.net/11441/68550
https://doi.org/10.1101/gr.202465.115
Access Level:acceso abierto
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spelling Novel features of telomere biology revealed by the absence of telomeric DNA methylationVega Vaquero, AlejandroBonoro, GiancarloMorselli, MarcoVaquero Sedas, María IsabelRubbi, LiudmillaPellegrini, M.Vega Palas, Miguel ÁngelCytosine methylation regulates the length and stability of telomeres, which can affect a wide variety of biological features, including cell differentiation, development, or illness. Although it is well established that subtelomeric regions are methylated, the presence of methylated cytosines at telomeres has remained controversial. Here, we have analyzed multiple bisulfite sequencing studies to address the methylation status of Arabidopsis thaliana telomeres. We found that the levels of estimated telomeric DNA methylation varied among studies. Interestingly, we estimated higher levels of telomeric DNA methylation in studies that produced C-rich telomeric strands with lower efficiency. However, these high methylation estimates arose due to experimental limitations of the bisulfite technique. We found a similar phenomenon for mitochondrial DNA: The levels of mitochondrial DNA methylation detected were higher in experiments with lower mitochondrial read production efficiencies. Based on experiments with high telomeric C-rich strand production efficiencies, we concluded that Arabidopsis telomeres are not methylated, which was confirmed by methylation-dependent restriction enzyme analyses. Thus, our studies indicate that telomeres are refractory to de novo DNA methylation by the RNA-directed DNA methylation machinery. This result, together with previously reported data, reveals that subtelomeric DNA methylation controls the homeostasis of telomere lengthCold Spring Harbor Laboratory PressBioquímica Vegetal y Biología Molecular2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/68550https://doi.org/10.1101/gr.202465.115reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésGenome Research, 26, 1047-1056.http://dx.doi.org/10.1101/gr.202465.115info:eu-repo/semantics/openAccessoai:idus.us.es:11441/685502026-06-17T12:51:07Z
dc.title.none.fl_str_mv Novel features of telomere biology revealed by the absence of telomeric DNA methylation
title Novel features of telomere biology revealed by the absence of telomeric DNA methylation
spellingShingle Novel features of telomere biology revealed by the absence of telomeric DNA methylation
Vega Vaquero, Alejandro
title_short Novel features of telomere biology revealed by the absence of telomeric DNA methylation
title_full Novel features of telomere biology revealed by the absence of telomeric DNA methylation
title_fullStr Novel features of telomere biology revealed by the absence of telomeric DNA methylation
title_full_unstemmed Novel features of telomere biology revealed by the absence of telomeric DNA methylation
title_sort Novel features of telomere biology revealed by the absence of telomeric DNA methylation
dc.creator.none.fl_str_mv Vega Vaquero, Alejandro
Bonoro, Giancarlo
Morselli, Marco
Vaquero Sedas, María Isabel
Rubbi, Liudmilla
Pellegrini, M.
Vega Palas, Miguel Ángel
author Vega Vaquero, Alejandro
author_facet Vega Vaquero, Alejandro
Bonoro, Giancarlo
Morselli, Marco
Vaquero Sedas, María Isabel
Rubbi, Liudmilla
Pellegrini, M.
Vega Palas, Miguel Ángel
author_role author
author2 Bonoro, Giancarlo
Morselli, Marco
Vaquero Sedas, María Isabel
Rubbi, Liudmilla
Pellegrini, M.
Vega Palas, Miguel Ángel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
description Cytosine methylation regulates the length and stability of telomeres, which can affect a wide variety of biological features, including cell differentiation, development, or illness. Although it is well established that subtelomeric regions are methylated, the presence of methylated cytosines at telomeres has remained controversial. Here, we have analyzed multiple bisulfite sequencing studies to address the methylation status of Arabidopsis thaliana telomeres. We found that the levels of estimated telomeric DNA methylation varied among studies. Interestingly, we estimated higher levels of telomeric DNA methylation in studies that produced C-rich telomeric strands with lower efficiency. However, these high methylation estimates arose due to experimental limitations of the bisulfite technique. We found a similar phenomenon for mitochondrial DNA: The levels of mitochondrial DNA methylation detected were higher in experiments with lower mitochondrial read production efficiencies. Based on experiments with high telomeric C-rich strand production efficiencies, we concluded that Arabidopsis telomeres are not methylated, which was confirmed by methylation-dependent restriction enzyme analyses. Thus, our studies indicate that telomeres are refractory to de novo DNA methylation by the RNA-directed DNA methylation machinery. This result, together with previously reported data, reveals that subtelomeric DNA methylation controls the homeostasis of telomere length
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/68550
https://doi.org/10.1101/gr.202465.115
url http://hdl.handle.net/11441/68550
https://doi.org/10.1101/gr.202465.115
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Genome Research, 26, 1047-1056.
http://dx.doi.org/10.1101/gr.202465.115
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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