Regulatory effects of dietary lipid sources on growth, physiological responses, fatty acid composition, and lipid metabolism in juvenile silver pomfret (Pampus argenteus)
A 70-day feeding trial was conducted to evaluate how different dietary lipid sources affect growth performance, physiological status, fatty acid deposition, and hepatic lipid metabolism in juvenile silver pomfret (Pampus argenteus) (initial weight: 2.92 ± 0.07 g). Four experimental diets, equal in p...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/407323 |
| Acceso en línea: | http://hdl.handle.net/10261/407323 |
| Access Level: | acceso embargado |
| Palabra clave: | Lipid sources Pampus argenteus Fatty acid composition Lipid metabolism Liver health |
| Sumario: | A 70-day feeding trial was conducted to evaluate how different dietary lipid sources affect growth performance, physiological status, fatty acid deposition, and hepatic lipid metabolism in juvenile silver pomfret (Pampus argenteus) (initial weight: 2.92 ± 0.07 g). Four experimental diets, equal in protein and lipid content, incorporated fish oil (FO), palm oil (PO), soybean oil (SO), and linseed oil (LO) as the main lipid source, respectively. FO and LO-fed fish achieved significantly greater final body weight (FW), weight gain (WG), specific growth rate (SGR), and feeding efficiency (FE) than those in fish fed PO. PO markedly elevated serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities while lowering glucose (GLU) level, suggesting hepatic impairment. PO also significantly reduced lipid content in both liver and muscle. Fatty acid profiles in tissues closely reflected dietary composition, with FO enhancing n-3 long-chain polyunsaturated fatty acids (LC-PUFAs) accumulation, SO and LO enriching n-6 and α-linolenic acid (ALA), respectively. Ultrastructural analysis revealed intact mitochondria and lipid droplets in FO, SO, and LO groups, while PO induced mitochondrial damage. SO upregulated lipogenetic metabolism genes (srebp-1c, accα, fas, and g6pd) expressions, while LO and PO upregulated lipolysis-related genes (pparα, lpl, atgl, and hsl) expressions. Overall, these results validate that dietary lipid sources influence the growth and hepatic health of silver pomfret by modulating fatty acid profiles, liver ultrastructure, and lipid metabolic pathways, with FO identified as the most favorable source for optimal growth and liver health in silver pomfret. |
|---|