Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles

Aquaculture is an extremely prosperous market threatened by pathogen outbreaks, including viruses as nodavirus (NNV), which infect fish species with special interest in trading such as European sea bass. Antimicrobial peptides (AMPs) might constitute potential antiviral agents, which had been previo...

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Detalles Bibliográficos
Autores: Cervera, Laura, Arizcun, Marta, Mercado, Luis, Chaves-Pozo, Elena, Cuesta, Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/352497
Acceso en línea:http://hdl.handle.net/10261/352497
https://api.elsevier.com/content/abstract/scopus_id/85183521003
Access Level:acceso abierto
Palabra clave:Antimicrobial peptides (AMPs)
Antivirals
Aquaculture
Dicentracin
European sea bass
Hepcidin
Nodavirus
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spelling Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory rolesCervera, LauraArizcun, MartaMercado, LuisChaves-Pozo, ElenaCuesta, AlbertoAntimicrobial peptides (AMPs)AntiviralsAquacultureDicentracinEuropean sea bassHepcidinNodavirusAquaculture is an extremely prosperous market threatened by pathogen outbreaks, including viruses as nodavirus (NNV), which infect fish species with special interest in trading such as European sea bass. Antimicrobial peptides (AMPs) might constitute potential antiviral agents, which had been previously evaluated in fish with positive prospects, based on their properties as immunomodulators or directly killing pathogens. In this line, we aimed to evaluate this dual role by administering two European sea bass synthetic AMPs (Hamp or Dic) prior to NNV infection. Both treatments conferred partial protection against NNV though viral replication and load were not affected. Both AMPs elicited, prior to infection, AMP response and leukocyte mobilization whilst down-regulated pro-inflammatory markers. Upon infection, Hamp and Dic peptides abrogated the inflammatory response provoked by NNV as well as avoid NNV-induced disturbance of the leucocyte distribution in the brain, mainly neutrophils, macrophages and CD8+ T cells. This study points that preventive applications of synthetic Hamp and Dic peptides exert their antiviral actions through the immunomodulatory role and not by a direct action of the antimicrobial on NNV. This work opens the door to the use of AMPs as potential prophylactic tools against NNV as well as immunostimulant in fish farms.This work was funded by Ministerio de Ciencia e Innovación-Agencia Estatal de Investigación (MCIN/AEI/10.13039/501100011033, grant PID2019-105522GB-I00 to A.C. and PRE2020-093771 to L.C.), Ministerio de Economía y Competitividad and FEDER (grant RTI2018-096625-BC33 to E.C.P. and M.A.).Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Ministerio de Economía y Competitividad (España)European CommissionArizcun, Marta [0000-0001-8514-1854]Chaves-Pozo, Elena [0000-0003-4030-1273]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/352497https://api.elsevier.com/content/abstract/scopus_id/85183521003reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105522GB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096625-B-C33The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.aquaculture.2024.740592https://doi.org/10.1016/j.aquaculture.2024.740592Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3524972026-05-22T06:33:51Z
dc.title.none.fl_str_mv Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles
title Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles
spellingShingle Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles
Cervera, Laura
Antimicrobial peptides (AMPs)
Antivirals
Aquaculture
Dicentracin
European sea bass
Hepcidin
Nodavirus
title_short Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles
title_full Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles
title_fullStr Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles
title_full_unstemmed Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles
title_sort Hepcidin and dicentracin peptides show preventive antiviral applications against NNV infection in European sea bass through immunomodulatory roles
dc.creator.none.fl_str_mv Cervera, Laura
Arizcun, Marta
Mercado, Luis
Chaves-Pozo, Elena
Cuesta, Alberto
author Cervera, Laura
author_facet Cervera, Laura
Arizcun, Marta
Mercado, Luis
Chaves-Pozo, Elena
Cuesta, Alberto
author_role author
author2 Arizcun, Marta
Mercado, Luis
Chaves-Pozo, Elena
Cuesta, Alberto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Ministerio de Economía y Competitividad (España)
European Commission
Arizcun, Marta [0000-0001-8514-1854]
Chaves-Pozo, Elena [0000-0003-4030-1273]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antimicrobial peptides (AMPs)
Antivirals
Aquaculture
Dicentracin
European sea bass
Hepcidin
Nodavirus
topic Antimicrobial peptides (AMPs)
Antivirals
Aquaculture
Dicentracin
European sea bass
Hepcidin
Nodavirus
description Aquaculture is an extremely prosperous market threatened by pathogen outbreaks, including viruses as nodavirus (NNV), which infect fish species with special interest in trading such as European sea bass. Antimicrobial peptides (AMPs) might constitute potential antiviral agents, which had been previously evaluated in fish with positive prospects, based on their properties as immunomodulators or directly killing pathogens. In this line, we aimed to evaluate this dual role by administering two European sea bass synthetic AMPs (Hamp or Dic) prior to NNV infection. Both treatments conferred partial protection against NNV though viral replication and load were not affected. Both AMPs elicited, prior to infection, AMP response and leukocyte mobilization whilst down-regulated pro-inflammatory markers. Upon infection, Hamp and Dic peptides abrogated the inflammatory response provoked by NNV as well as avoid NNV-induced disturbance of the leucocyte distribution in the brain, mainly neutrophils, macrophages and CD8+ T cells. This study points that preventive applications of synthetic Hamp and Dic peptides exert their antiviral actions through the immunomodulatory role and not by a direct action of the antimicrobial on NNV. This work opens the door to the use of AMPs as potential prophylactic tools against NNV as well as immunostimulant in fish farms.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/352497
https://api.elsevier.com/content/abstract/scopus_id/85183521003
url http://hdl.handle.net/10261/352497
https://api.elsevier.com/content/abstract/scopus_id/85183521003
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105522GB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096625-B-C33
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.aquaculture.2024.740592
https://doi.org/10.1016/j.aquaculture.2024.740592
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
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