Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.

A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these habitats. In this work, active and inactive insertion...

Descripción completa

Detalles Bibliográficos
Autores: Blesa, Alba, Sánchez, Mercedes, Sacristán-Horcajada, Eva, de la Fuente, Sandra González, Peiró, Ramón, Berenguer Carlos, José
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/690104
Acceso en línea:http://hdl.handle.net/10486/690104
https://dx.doi.org/10.3390/microorganisms7010025
Access Level:acceso abierto
Palabra clave:HGT
Insertion sequence
Mobilome
Thermophiles
Thermus
Transposases
Transposons
Biología y Biomedicina / Biología
id ES_0c06fa93f56b0158fe1d3bcdf35aebda
oai_identifier_str oai:repositorio.uam.es:10486/690104
network_acronym_str ES
network_name_str España
repository_id_str
spelling Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.Blesa, AlbaSánchez, MercedesSacristán-Horcajada, Evade la Fuente, Sandra GonzálezPeiró, RamónBerenguer Carlos, JoséHGTInsertion sequenceMobilomeThermophilesThermusTransposasesTransposonsBiología y Biomedicina / BiologíaA high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these habitats. In this work, active and inactive insertion sequences (ISs) spanning the sequenced members of the genus Thermus were characterized, with special emphasis on three T. thermophilus strains: HB27, HB8, and NAR1. A large number of full ISs and fragments derived from different IS families were found, concentrating within megaplasmids present in most isolates. Potentially active ISs were identified through analysis of transposase integrity, and domestication-related transposition events of ISTth7 were identified in laboratory-adapted HB27 derivatives. Many partial copies of ISs appeared throughout the genome, which may serve as specific targets for homologous recombination contributing to genome rearrangement. Moreover, recruitment of IS1000 32 bp segments as spacers for CRISPR sequence was identified, pointing to the adaptability of these elements in the biology of these thermophiles. Further knowledge about the activity and functional diversity of ISs in this genus may contribute to the generation of engineered transposons as new genetic tools, and enrich our understanding of the outstanding plasticity shown by these thermophilesThis work was supported by grants BIO2016-77031-R from the Spanish Ministry of Economy and Competitiveness and grant nº 685474 from Horizon 2020 research and innovation program of the European Union. An institutional grant from Fundación Ramón Areces to the CBMSO is also acknowledgedMDPI AGFacultad de CienciasCentro de Biología Molecular Severo Ochoa (CBM)20192019-01-21research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/690104https://dx.doi.org/10.3390/microorganisms7010025reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6901042026-06-23T12:46:27Z
dc.title.none.fl_str_mv Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
title Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
spellingShingle Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
Blesa, Alba
HGT
Insertion sequence
Mobilome
Thermophiles
Thermus
Transposases
Transposons
Biología y Biomedicina / Biología
title_short Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
title_full Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
title_fullStr Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
title_full_unstemmed Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
title_sort Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
dc.creator.none.fl_str_mv Blesa, Alba
Sánchez, Mercedes
Sacristán-Horcajada, Eva
de la Fuente, Sandra González
Peiró, Ramón
Berenguer Carlos, José
author Blesa, Alba
author_facet Blesa, Alba
Sánchez, Mercedes
Sacristán-Horcajada, Eva
de la Fuente, Sandra González
Peiró, Ramón
Berenguer Carlos, José
author_role author
author2 Sánchez, Mercedes
Sacristán-Horcajada, Eva
de la Fuente, Sandra González
Peiró, Ramón
Berenguer Carlos, José
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Facultad de Ciencias
Centro de Biología Molecular Severo Ochoa (CBM)
dc.subject.none.fl_str_mv HGT
Insertion sequence
Mobilome
Thermophiles
Thermus
Transposases
Transposons
Biología y Biomedicina / Biología
topic HGT
Insertion sequence
Mobilome
Thermophiles
Thermus
Transposases
Transposons
Biología y Biomedicina / Biología
description A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these habitats. In this work, active and inactive insertion sequences (ISs) spanning the sequenced members of the genus Thermus were characterized, with special emphasis on three T. thermophilus strains: HB27, HB8, and NAR1. A large number of full ISs and fragments derived from different IS families were found, concentrating within megaplasmids present in most isolates. Potentially active ISs were identified through analysis of transposase integrity, and domestication-related transposition events of ISTth7 were identified in laboratory-adapted HB27 derivatives. Many partial copies of ISs appeared throughout the genome, which may serve as specific targets for homologous recombination contributing to genome rearrangement. Moreover, recruitment of IS1000 32 bp segments as spacers for CRISPR sequence was identified, pointing to the adaptability of these elements in the biology of these thermophiles. Further knowledge about the activity and functional diversity of ISs in this genus may contribute to the generation of engineered transposons as new genetic tools, and enrich our understanding of the outstanding plasticity shown by these thermophiles
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-21
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/690104
https://dx.doi.org/10.3390/microorganisms7010025
url http://hdl.handle.net/10486/690104
https://dx.doi.org/10.3390/microorganisms7010025
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403261406019584
score 15,300719

Similares en LA Referencia