Cyclic multiplex fluorescent immunohistochemistry and machine learning reveal distinct states of astrocytes and microglia in normal aging and Alzheimer's disease

[Background] Astrocytes and microglia react to Aβ plaques, neurofibrillary tangles, and neurodegeneration in the Alzheimer's disease (AD) brain. Single-nuclei and single-cell RNA-seq have revealed multiple states or subpopulations of these glial cells but lack spatial information. We have devel...

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Detalhes bibliográficos
Autores: Muñoz-Castro, Clara, Noori, Ayush, Magdamo, Colin G., Li, Zhaozhi, Marks, Jordan D., Frosch, Matthew P., Das, Sudeshna, Hyman, Bradley T., Serrano-Pozo, Alberto
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/305309
Acesso em linha:http://hdl.handle.net/10261/305309
https://api.elsevier.com/content/abstract/scopus_id/85123973861
Access Level:acceso abierto
Palavra-chave:Alzheimer’s disease
Amyloid plaques
Astrocytes
Immunohistochemistry
Microglia
Neurofibrillary tangles
Neuropathology
Tau
Descrição
Resumo:[Background] Astrocytes and microglia react to Aβ plaques, neurofibrillary tangles, and neurodegeneration in the Alzheimer's disease (AD) brain. Single-nuclei and single-cell RNA-seq have revealed multiple states or subpopulations of these glial cells but lack spatial information. We have developed a methodology of cyclic multiplex fluorescent immunohistochemistry on human postmortem brains and image analysis that enables a comprehensive morphological quantitative characterization of astrocytes and microglia in the context of their spatial relationships with plaques and tangles.

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