Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

PMCID: PMC2654259 NIHMSID: NIHMS96070

Detalles Bibliográficos
Autores: Klionsky, Daniel J., Deter, Russell L., González García, Ramón, Cebollero, Eduardo
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/41229
Acceso en línea:http://hdl.handle.net/10261/41229
Access Level:acceso abierto
Palabra clave:Autolysosome
Autophagosomes
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spelling Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotesKlionsky, Daniel J.Deter, Russell L.González García, RamónCebollero, EduardoAutolysosomeAutophagosomesPMCID: PMC2654259 NIHMSID: NIHMS96070Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.The authors of this manuscript support the guidelines described herein. We thank Heather Pua (Duke University Medical Center) and Drs. Daniel Rigden (University of Liverpool) and David Russell (Cornell University College of Veterinary Medicine) for reading the manuscript and providing helpful comments. D.J.K. extends his sincere apologies to researchers in the field of autophagy who, due to oversight or any other reason, could not be included on this manuscript. This work was supported by National Institutes of Health Public Health Service grant GM53396 to D.J.K. Due to space and other limitations, it is not possible to include all other sources of financial support.Peer reviewedNational Institutes of Health (U.S.). PubMed Central201120112008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/41229reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.landesbioscience.com/journals/autophagy/article/5338/?nocache=956794090info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/412292026-05-22T06:33:51Z
dc.title.none.fl_str_mv Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
title Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
spellingShingle Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
Klionsky, Daniel J.
Autolysosome
Autophagosomes
title_short Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
title_full Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
title_fullStr Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
title_full_unstemmed Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
title_sort Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
dc.creator.none.fl_str_mv Klionsky, Daniel J.
Deter, Russell L.
González García, Ramón
Cebollero, Eduardo
author Klionsky, Daniel J.
author_facet Klionsky, Daniel J.
Deter, Russell L.
González García, Ramón
Cebollero, Eduardo
author_role author
author2 Deter, Russell L.
González García, Ramón
Cebollero, Eduardo
author2_role author
author
author
dc.subject.none.fl_str_mv Autolysosome
Autophagosomes
topic Autolysosome
Autophagosomes
description PMCID: PMC2654259 NIHMSID: NIHMS96070
publishDate 2008
dc.date.none.fl_str_mv 2008
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/41229
url http://hdl.handle.net/10261/41229
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.landesbioscience.com/journals/autophagy/article/5338/?nocache=956794090
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Institutes of Health (U.S.). PubMed Central
publisher.none.fl_str_mv National Institutes of Health (U.S.). PubMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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