omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells

We have studied the contribution of P-type voltage-dependent Ca2+ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K+ or nicotine using omega-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) i...

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Detalhes bibliográficos
Autores: Granja, Ricardo, Fernández-Fernández, José Manuel, 1967-, Izaguirre, Victor, González García, Carmen, Ceña, Valentin
Formato: artículo
Estado:Versión publicada
Fecha de publicación:1995
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/25632
Acesso em linha:http://hdl.handle.net/10230/25632
http://dx.doi.org/10.1016/0014-5793(95)00149-4
Access Level:acceso abierto
Palavra-chave:Canals de potassi
Nicotina
Calcium channel
P-type
Nicotinic receptor
omega-Agatoxin IVA
Secretion
Chromaffin cell
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repository_id_str
spelling omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cellsGranja, RicardoFernández-Fernández, José Manuel, 1967-Izaguirre, VictorGonzález García, CarmenCeña, ValentinCanals de potassiNicotinaCalcium channelP-typeNicotinic receptoromega-Agatoxin IVASecretionChromaffin cellWe have studied the contribution of P-type voltage-dependent Ca2+ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K+ or nicotine using omega-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced the release of about 13% of norepinephrine, 5% epinephrine and 11% ATP, and that omega-agatoxin (100 nM) did not affect this secretion. However, both nicotine-induced CA and ATP secretion were significantly blocked (about 50%) by omega-agatoxin IVA (100 nM). In addition, this toxin also reversibly blocked (about 70%) the inward current induced by nicotine in bovine chromaffin cells. The results suggest that, besides its known action of blocking P-type voltage-dependent channels, omega-agatoxin is a potent and reversible blocker of the nicotinic receptor channel in chromaffin cells, and that this action would explain the blockade of nicotine-induced secretion.This work was supported, in part, by grants from Fundación Salud 2000, Dirección General de Investigación Cientifica y Técnica, Grant PM92-0112, and the European Economic Community, Grant SC1"-CT91-0709. R.G. was supported by a University of Alicante fellowship, J.M.F. by a CAM fellowship, and V.I. by a Generalitat Valenciana fellowship.Elsevier201620161995info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/25632http://dx.doi.org/10.1016/0014-5793(95)00149-4reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFEBS letters. 1995;362(1):15-8© Elsevier This is the published version of an article http://dx.doi.org/10.1016/0014-5793(95)00149-4 that appeared in the journal Developmental cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseinfo:eu-repo/semantics/openAccessoai:recercat.cat:10230/256322026-05-29T05:05:01Z
dc.title.none.fl_str_mv omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells
title omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells
spellingShingle omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells
Granja, Ricardo
Canals de potassi
Nicotina
Calcium channel
P-type
Nicotinic receptor
omega-Agatoxin IVA
Secretion
Chromaffin cell
title_short omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells
title_full omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells
title_fullStr omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells
title_full_unstemmed omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells
title_sort omega-Agatoxin IVA blocks nicotinic receptor channels in bovine chromaffin cells
dc.creator.none.fl_str_mv Granja, Ricardo
Fernández-Fernández, José Manuel, 1967-
Izaguirre, Victor
González García, Carmen
Ceña, Valentin
author Granja, Ricardo
author_facet Granja, Ricardo
Fernández-Fernández, José Manuel, 1967-
Izaguirre, Victor
González García, Carmen
Ceña, Valentin
author_role author
author2 Fernández-Fernández, José Manuel, 1967-
Izaguirre, Victor
González García, Carmen
Ceña, Valentin
author2_role author
author
author
author
dc.subject.none.fl_str_mv Canals de potassi
Nicotina
Calcium channel
P-type
Nicotinic receptor
omega-Agatoxin IVA
Secretion
Chromaffin cell
topic Canals de potassi
Nicotina
Calcium channel
P-type
Nicotinic receptor
omega-Agatoxin IVA
Secretion
Chromaffin cell
description We have studied the contribution of P-type voltage-dependent Ca2+ channels to both catacholamine (CA) and ATP secretion from bovine chromaffin cells induced by high K+ or nicotine using omega-agatoxin IVA, a selective blocker of P-type voltage-dependent Ca2+ channels. We found that high K+ (75 mM) induced the release of about 13% of norepinephrine, 5% epinephrine and 11% ATP, and that omega-agatoxin (100 nM) did not affect this secretion. However, both nicotine-induced CA and ATP secretion were significantly blocked (about 50%) by omega-agatoxin IVA (100 nM). In addition, this toxin also reversibly blocked (about 70%) the inward current induced by nicotine in bovine chromaffin cells. The results suggest that, besides its known action of blocking P-type voltage-dependent channels, omega-agatoxin is a potent and reversible blocker of the nicotinic receptor channel in chromaffin cells, and that this action would explain the blockade of nicotine-induced secretion.
publishDate 1995
dc.date.none.fl_str_mv 1995
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/25632
http://dx.doi.org/10.1016/0014-5793(95)00149-4
url http://hdl.handle.net/10230/25632
http://dx.doi.org/10.1016/0014-5793(95)00149-4
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv FEBS letters. 1995;362(1):15-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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