Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves

Green chemistry focuses on reducing the environmental impacts of chemicals through sustainable practices. Traditional methods for extracting bioactive compounds from Eucalyptus marginata leaves, such as hydro-distillation and organic solvent extraction, have limitations, including long extraction ti...

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Autores: Hasni, Soumaya, Riguene, Hajer, Mendiola, J. A., Ibáñez, Elena, Montero, Lidia, Domínguez-Rodríguez, Gloria, Ghazghazi, Hanene, Rigane, Ghayth, Salem, Ridha Ben
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/375505
Acesso em linha:http://hdl.handle.net/10261/375505
Access Level:Acceso aberto
Palavra-chave:Eucalyptus marginata
Supercritical fluid extraction
Green solvent
Acetylcholinesterase inhibition
Phenolic compounds
GC-MS analysis
LC-ESI-MS/MS
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spelling Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leavesHasni, SoumayaRiguene, HajerMendiola, J. A.Ibáñez, ElenaMontero, LidiaDomínguez-Rodríguez, GloriaGhazghazi, HaneneRigane, GhaythSalem, Ridha BenEucalyptus marginataSupercritical fluid extractionGreen solventAcetylcholinesterase inhibitionPhenolic compoundsGC-MS analysisLC-ESI-MS/MSGreen chemistry focuses on reducing the environmental impacts of chemicals through sustainable practices. Traditional methods for extracting bioactive compounds from Eucalyptus marginata leaves, such as hydro-distillation and organic solvent extraction, have limitations, including long extraction times, high energy consumption, and potential toxic solvent residues. This study explored the use of supercritical fluid extraction (SFE), pressurized liquid extraction (PLE), and gas-expanded liquid (GXL) processes to improve efficiency and selectivity. These techniques were combined in a single mixture design, where CO2 was used in the experiments carried out under SFE, while water and ethanol were used for the PLE and GXL experiments by varying the concentration of the solvents to cover all the extraction possibilities. The neuroprotective activity of the extracts was evaluated by measuring their antioxidant, anti-inflammatory, and acetylcholinesterase inhibition properties. The optimization resulted in a novel GXL extraction with an optimal ternary mixture of 27% CO2, 55% ethanol, and 18% water, with a high degree of desirability (R2 = 88.59%). Chromatographic analysis carried out by GC-MS and HPLC-ESI-MS/MS identified over 49 metabolites. The designed sustainable extraction process offers a promising approach for producing phenolic-rich plant extracts in industrial applications.We thank the Ministry of Higher Education and Scientific Research, Tunisia. L.M. acknowledges a “Ramon y Cajal” grant (RYC2021-033148-I) funded by MCIN/AEI/10.13039/501100011033 and by “European Union Next Generation EU/PRTR”. This research has been financially supported by project PID2020-113050RB-I00, under the auspices of the Agencia Estatal de Investigación (AEI) from Ministerio de Ciencia e Innovación (MICINN, Government of Spain) and by project COOPA22033, financed by the Spanish National Research Council (CSIC).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas (España)Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/375505reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113050RB-I00info:eu-repo/grantAgreement/AEI//RYC2021-033148-IThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/ijms26010094https://doi.org/10.3390/ijms26010094Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3755052026-05-22T06:33:51Z
dc.title.none.fl_str_mv Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves
title Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves
spellingShingle Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves
Hasni, Soumaya
Eucalyptus marginata
Supercritical fluid extraction
Green solvent
Acetylcholinesterase inhibition
Phenolic compounds
GC-MS analysis
LC-ESI-MS/MS
title_short Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves
title_full Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves
title_fullStr Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves
title_full_unstemmed Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves
title_sort Optimization of a pressurized extraction process based on a ternary solvent system for the recovery of neuroprotective compounds from Eucalyptus marginata leaves
dc.creator.none.fl_str_mv Hasni, Soumaya
Riguene, Hajer
Mendiola, J. A.
Ibáñez, Elena
Montero, Lidia
Domínguez-Rodríguez, Gloria
Ghazghazi, Hanene
Rigane, Ghayth
Salem, Ridha Ben
author Hasni, Soumaya
author_facet Hasni, Soumaya
Riguene, Hajer
Mendiola, J. A.
Ibáñez, Elena
Montero, Lidia
Domínguez-Rodríguez, Gloria
Ghazghazi, Hanene
Rigane, Ghayth
Salem, Ridha Ben
author_role author
author2 Riguene, Hajer
Mendiola, J. A.
Ibáñez, Elena
Montero, Lidia
Domínguez-Rodríguez, Gloria
Ghazghazi, Hanene
Rigane, Ghayth
Salem, Ridha Ben
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Eucalyptus marginata
Supercritical fluid extraction
Green solvent
Acetylcholinesterase inhibition
Phenolic compounds
GC-MS analysis
LC-ESI-MS/MS
topic Eucalyptus marginata
Supercritical fluid extraction
Green solvent
Acetylcholinesterase inhibition
Phenolic compounds
GC-MS analysis
LC-ESI-MS/MS
description Green chemistry focuses on reducing the environmental impacts of chemicals through sustainable practices. Traditional methods for extracting bioactive compounds from Eucalyptus marginata leaves, such as hydro-distillation and organic solvent extraction, have limitations, including long extraction times, high energy consumption, and potential toxic solvent residues. This study explored the use of supercritical fluid extraction (SFE), pressurized liquid extraction (PLE), and gas-expanded liquid (GXL) processes to improve efficiency and selectivity. These techniques were combined in a single mixture design, where CO2 was used in the experiments carried out under SFE, while water and ethanol were used for the PLE and GXL experiments by varying the concentration of the solvents to cover all the extraction possibilities. The neuroprotective activity of the extracts was evaluated by measuring their antioxidant, anti-inflammatory, and acetylcholinesterase inhibition properties. The optimization resulted in a novel GXL extraction with an optimal ternary mixture of 27% CO2, 55% ethanol, and 18% water, with a high degree of desirability (R2 = 88.59%). Chromatographic analysis carried out by GC-MS and HPLC-ESI-MS/MS identified over 49 metabolites. The designed sustainable extraction process offers a promising approach for producing phenolic-rich plant extracts in industrial applications.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/375505
url http://hdl.handle.net/10261/375505
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113050RB-I00
info:eu-repo/grantAgreement/AEI//RYC2021-033148-I
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/ijms26010094
https://doi.org/10.3390/ijms26010094
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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