Easy introduction of maleimides at different positions of oligonucleotide chains for conjugation purposes
[2,5-Dimethylfuran]-protected maleimides were placed at both internal positions and the 3'-end of oligonucleotides making use of solid-phase synthesis procedures. A new phosphoramidite derivative and a new solid support incorporating the protected maleimide moiety were prepared for this purpose...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/49472 |
| Acceso en línea: | https://hdl.handle.net/2445/49472 |
| Access Level: | acceso abierto |
| Palabra clave: | Biotecnologia Àcids nucleics Espectrometria de masses Nucleòtids Compostos heterocíclics Biotechnology Nucleic acids Mass spectrometry Nucleotides Heterocyclic compounds |
| Sumario: | [2,5-Dimethylfuran]-protected maleimides were placed at both internal positions and the 3'-end of oligonucleotides making use of solid-phase synthesis procedures. A new phosphoramidite derivative and a new solid support incorporating the protected maleimide moiety were prepared for this purpose. In all cases maleimide deprotection (retro-Diels-Alder reaction) followed by reaction with thiol-containing compounds afforded the target conjugate. |
|---|