Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance

19 p.-8 fig.-5 tab.

Detalhes bibliográficos
Autores: Rodríguez de Córdoba, Santiago, Reparaz, Andrea, Sánchez, Daniel, Pinto, Sheila, López, Lucia Juana, Martín Merinero, Héctor, Calvete, Iria, Pérez-Pérez, Julián, Jellison, Sidney S., Zhang, Yuzhou, Smith, Richard, Moreno, Inmaculada, Domínguez, Mercedes
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/380641
Acesso em linha:http://hdl.handle.net/10261/380641
Access Level:acceso abierto
Palavra-chave:Anti factor H autoantibodies
Immunochromatographic test
Complement
Atypical hemolytic uremic syndrome
C3-glomerulopathy
Diagnostic test
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spelling Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevanceRodríguez de Córdoba, SantiagoReparaz, AndreaSánchez, DanielPinto, SheilaLópez, Lucia JuanaMartín Merinero, HéctorCalvete, IriaPérez-Pérez, JuliánJellison, Sidney S.Zhang, YuzhouSmith, RichardMoreno, InmaculadaDomínguez, MercedesAnti factor H autoantibodiesImmunochromatographic testComplementAtypical hemolytic uremic syndromeC3-glomerulopathyDiagnostic test19 p.-8 fig.-5 tab.Factor H (FH) is a crucial complement regulator that prevents complement-mediated injury to healthy cells and tissues. This regulatory function can be disrupted by Factor H autoantibodies (FHAA), which then leads to diseases such as atypical hemolytic uremic syndrome (aHUS) and C3 Glomerulopathy (C3G). In pediatric aHUS, the FHAA incidence is ~10-15%, although in the Indian population, it rises to ~50%. The specific regions of FH targeted by FHAAs correlate with the pathogenic mechanism of the associated disease. In aHUS, FHAAs target the C-terminus, thereby impacting FH ability to recognize cell surfaces. In C3G, in contrast, FHAAs often target the N-terminus, generating an acquired functional FH deficiency. Detection and monitoring FHAAs are decisive for effectively treating patients. Current FHAA analysis normally identify free FHAAs that bind surface-bound FH using ELISA techniques. These methods require well-equipped laboratories and qualified staff, and do not measure FH-FHAA complexes, which can make it difficult to correlate titers with clinical outcomes. The visually-based immunochromatographic test (ICT) described herein allows for quick detection and quantification of IgG and IgM FH-FHAA complexes in human EDTA-plasma or serum. This ICT offers improved detection of FHAAs compared to ELISA as demonstrated by cases where the ICT identifies FH-FHAA complexes in samples that tested negative with the free FHAA ELISA. Importantly, the ICT indirectly informs on the amount of FH that is complexed with FHAAs, thus assessing the significance of the FHAA in disrupting the regulatory function of FH. Overall, this novel assay offers a simple, fast, cost-effective, and, likely, more clinically relevant alternative for diagnosing FHAAs in at-risk populations.The author(s) declare financial support was received for the research, authorship, and/or publication of this article. SR was supported by grants from the Spanish Ministerio de Economía y Competitividad-FEDER (PID2019-104912RB-I00) and Autonomous Region of Madrid (S2022/BMD-7278). RS was supported in part by a grant from the National Institutes of Health (R01 DK110023).Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)Comunidad de MadridNational Institutes of Health (US)Rodríguez de Córdoba, Santiago [0000-0001-6401-1874]Reparaz, Andrea [0000-0003-3751-7743]Pinto, Sheila [0000-0002-6475-9740]Martín Merinero, Héctor [0000-0002-9094-5934]Pérez-Pérez, Julián [0000-0001-6091-0012]Smith, Richard [0000-0003-1201-6731]Moreno, Inmaculada [0000-0001-5001-3764]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/380641reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104912RB-I00S2022/BMD-7278-COMPLEMENTO 3-CMhttps://doi.org/10.3389/fimmu.2024.1527016Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3806412026-05-22T06:33:51Z
dc.title.none.fl_str_mv Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance
title Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance
spellingShingle Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance
Rodríguez de Córdoba, Santiago
Anti factor H autoantibodies
Immunochromatographic test
Complement
Atypical hemolytic uremic syndrome
C3-glomerulopathy
Diagnostic test
title_short Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance
title_full Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance
title_fullStr Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance
title_full_unstemmed Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance
title_sort Novel immunochromatographic test for rapid detection of anti-factor H autoantibodies with an assessment of its clinical relevance
dc.creator.none.fl_str_mv Rodríguez de Córdoba, Santiago
Reparaz, Andrea
Sánchez, Daniel
Pinto, Sheila
López, Lucia Juana
Martín Merinero, Héctor
Calvete, Iria
Pérez-Pérez, Julián
Jellison, Sidney S.
Zhang, Yuzhou
Smith, Richard
Moreno, Inmaculada
Domínguez, Mercedes
author Rodríguez de Córdoba, Santiago
author_facet Rodríguez de Córdoba, Santiago
Reparaz, Andrea
Sánchez, Daniel
Pinto, Sheila
López, Lucia Juana
Martín Merinero, Héctor
Calvete, Iria
Pérez-Pérez, Julián
Jellison, Sidney S.
Zhang, Yuzhou
Smith, Richard
Moreno, Inmaculada
Domínguez, Mercedes
author_role author
author2 Reparaz, Andrea
Sánchez, Daniel
Pinto, Sheila
López, Lucia Juana
Martín Merinero, Héctor
Calvete, Iria
Pérez-Pérez, Julián
Jellison, Sidney S.
Zhang, Yuzhou
Smith, Richard
Moreno, Inmaculada
Domínguez, Mercedes
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
National Institutes of Health (US)
Rodríguez de Córdoba, Santiago [0000-0001-6401-1874]
Reparaz, Andrea [0000-0003-3751-7743]
Pinto, Sheila [0000-0002-6475-9740]
Martín Merinero, Héctor [0000-0002-9094-5934]
Pérez-Pérez, Julián [0000-0001-6091-0012]
Smith, Richard [0000-0003-1201-6731]
Moreno, Inmaculada [0000-0001-5001-3764]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anti factor H autoantibodies
Immunochromatographic test
Complement
Atypical hemolytic uremic syndrome
C3-glomerulopathy
Diagnostic test
topic Anti factor H autoantibodies
Immunochromatographic test
Complement
Atypical hemolytic uremic syndrome
C3-glomerulopathy
Diagnostic test
description 19 p.-8 fig.-5 tab.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/380641
url http://hdl.handle.net/10261/380641
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104912RB-I00
S2022/BMD-7278-COMPLEMENTO 3-CM
https://doi.org/10.3389/fimmu.2024.1527016
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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