Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers

Micronuclei, comet and chromosome alterations assays are the most widely used biomarkers for determining the genotoxic damage in a population exposed to genotoxic chemicals. While chromosome alterations are an excellent biomarker to detect short- and long-term genotoxic effects, the comet assay only...

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Autores: Biern, Gloria, Giraldo, Jesús, Zock, Jan-Paul, Monyarch, Gemma, Espinosa Cardiel, Ana, Rodríguez-Trigo, Gema, Gómez, Federico Pablo, Pozo-Rodríguez, Francisco, Barberà i Mir, Joan Albert, Fuster, Carme
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/102524
Acesso em linha:https://hdl.handle.net/2445/102524
Access Level:acceso abierto
Palavra-chave:Mutació (Biologia)
Contaminants
Toxicologia
Petroli
Catàstrofe del Prestige, Espanya, 2002
Mutation (Biology)
Pollutants
Toxicology
Petroleum
Prestige oil spill, Spain, 2002
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spelling Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkersBiern, GloriaGiraldo, JesúsZock, Jan-PaulMonyarch, GemmaEspinosa Cardiel, AnaRodríguez-Trigo, GemaGómez, Federico PabloPozo-Rodríguez, FranciscoBarberà i Mir, Joan AlbertFuster, CarmeMutació (Biologia)ContaminantsToxicologiaPetroliCatàstrofe del Prestige, Espanya, 2002Mutation (Biology)PollutantsToxicologyPetroleumPrestige oil spill, Spain, 2002Micronuclei, comet and chromosome alterations assays are the most widely used biomarkers for determining the genotoxic damage in a population exposed to genotoxic chemicals. While chromosome alterations are an excellent biomarker to detect short- and long-term genotoxic effects, the comet assay only measures early biological effects, and furthermore it is unknown whether nuclear abnormalies, such as those measured in the micronucleus test, remain detectable long-term after an acute exposure. In our previous study, an increase in structural chromosome alterations in fishermen involved in the clean-up of the Prestige oil spill, two years after acute exposure, was detected. The aim of this study is to investigate whether, in lymphocytes from peripheral blood, the nuclear abnormalies (micronucleus, nucleoplasmic bridges and nuclear buds) have a similar sensitivity to the chromosome damage analysis for genotoxic detection two years after oil exposure in the same non-smoker individuals and in the same peripheral blood extraction. No significant differences in nuclear abnormalies frequencies between exposed and non-exposed individuals were found (p > 0.05). However, chromosome damage, in the same individuals, was higher in exposed vs. non-exposed individuals, especially for chromosome lesions (p < 0.05). These findings, despite the small sample size, suggest that nuclear abnormalities are probably less-successful biomarkers than are chromosome alterations to evaluate genotoxic effects two or more years after an exposure to oil. Due to the great advantage of micronucleus automatic determination, which allows for a rapid study of hundreds of individuals exposed to genotoxic chemical exposure, further studies are needed to confirm whether this assay is or is not useful in long-term genotoxic studies after the toxic agent is no longer present.MDPI2016201620152016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfhttps://hdl.handle.net/2445/102524Articles publicats en revistes (Medicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.3390/jmse3041334Journal of Marine Science and Engineering, 2015, vol. 3, p. 1334-1348http://dx.doi.org/10.3390/jmse3041334cc-by (c) Biern, G. et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1025242026-05-29T05:05:01Z
dc.title.none.fl_str_mv Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
title Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
spellingShingle Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
Biern, Gloria
Mutació (Biologia)
Contaminants
Toxicologia
Petroli
Catàstrofe del Prestige, Espanya, 2002
Mutation (Biology)
Pollutants
Toxicology
Petroleum
Prestige oil spill, Spain, 2002
title_short Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
title_full Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
title_fullStr Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
title_full_unstemmed Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
title_sort Human genotoxic study carried out two years after oil exposure during the clean-up activities using two different biomarkers
dc.creator.none.fl_str_mv Biern, Gloria
Giraldo, Jesús
Zock, Jan-Paul
Monyarch, Gemma
Espinosa Cardiel, Ana
Rodríguez-Trigo, Gema
Gómez, Federico Pablo
Pozo-Rodríguez, Francisco
Barberà i Mir, Joan Albert
Fuster, Carme
author Biern, Gloria
author_facet Biern, Gloria
Giraldo, Jesús
Zock, Jan-Paul
Monyarch, Gemma
Espinosa Cardiel, Ana
Rodríguez-Trigo, Gema
Gómez, Federico Pablo
Pozo-Rodríguez, Francisco
Barberà i Mir, Joan Albert
Fuster, Carme
author_role author
author2 Giraldo, Jesús
Zock, Jan-Paul
Monyarch, Gemma
Espinosa Cardiel, Ana
Rodríguez-Trigo, Gema
Gómez, Federico Pablo
Pozo-Rodríguez, Francisco
Barberà i Mir, Joan Albert
Fuster, Carme
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mutació (Biologia)
Contaminants
Toxicologia
Petroli
Catàstrofe del Prestige, Espanya, 2002
Mutation (Biology)
Pollutants
Toxicology
Petroleum
Prestige oil spill, Spain, 2002
topic Mutació (Biologia)
Contaminants
Toxicologia
Petroli
Catàstrofe del Prestige, Espanya, 2002
Mutation (Biology)
Pollutants
Toxicology
Petroleum
Prestige oil spill, Spain, 2002
description Micronuclei, comet and chromosome alterations assays are the most widely used biomarkers for determining the genotoxic damage in a population exposed to genotoxic chemicals. While chromosome alterations are an excellent biomarker to detect short- and long-term genotoxic effects, the comet assay only measures early biological effects, and furthermore it is unknown whether nuclear abnormalies, such as those measured in the micronucleus test, remain detectable long-term after an acute exposure. In our previous study, an increase in structural chromosome alterations in fishermen involved in the clean-up of the Prestige oil spill, two years after acute exposure, was detected. The aim of this study is to investigate whether, in lymphocytes from peripheral blood, the nuclear abnormalies (micronucleus, nucleoplasmic bridges and nuclear buds) have a similar sensitivity to the chromosome damage analysis for genotoxic detection two years after oil exposure in the same non-smoker individuals and in the same peripheral blood extraction. No significant differences in nuclear abnormalies frequencies between exposed and non-exposed individuals were found (p > 0.05). However, chromosome damage, in the same individuals, was higher in exposed vs. non-exposed individuals, especially for chromosome lesions (p < 0.05). These findings, despite the small sample size, suggest that nuclear abnormalities are probably less-successful biomarkers than are chromosome alterations to evaluate genotoxic effects two or more years after an exposure to oil. Due to the great advantage of micronucleus automatic determination, which allows for a rapid study of hundreds of individuals exposed to genotoxic chemical exposure, further studies are needed to confirm whether this assay is or is not useful in long-term genotoxic studies after the toxic agent is no longer present.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/102524
url https://hdl.handle.net/2445/102524
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.3390/jmse3041334
Journal of Marine Science and Engineering, 2015, vol. 3, p. 1334-1348
http://dx.doi.org/10.3390/jmse3041334
dc.rights.none.fl_str_mv cc-by (c) Biern, G. et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Biern, G. et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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