Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells

Several studies have demonstrated that rosemary polyphenols exert changes in the lipid metabolism in adipose and hepatic cells. In this work, the effects of a polyphenol-enriched supercritical rosemary extract (SC-RE) and carnosic acid (CA) on the transcriptome and cholesterol metabolism in HT-29 co...

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Authors: Valdés, Alberto, Sullini, Giuseppe, Ibáñez, Elena, Cifuentes, Alejandro, García-Cañas, Virginia
Format: article
Status:Versión aceptada para publicación
Publication Date:2015
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/149960
Online Access:http://hdl.handle.net/10261/149960
Access Level:Open access
Keyword:Transcriptomics
Colon cancer
Foodomics
Rosemary
Unfolded protein response
Carnosic acid
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spelling Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cellsValdés, AlbertoSullini, GiuseppeIbáñez, ElenaCifuentes, AlejandroGarcía-Cañas, VirginiaTranscriptomicsColon cancerFoodomicsRosemaryUnfolded protein responseCarnosic acidSeveral studies have demonstrated that rosemary polyphenols exert changes in the lipid metabolism in adipose and hepatic cells. In this work, the effects of a polyphenol-enriched supercritical rosemary extract (SC-RE) and carnosic acid (CA) on the transcriptome and cholesterol metabolism in HT-29 colon cancer cells were examined using a Foodomics approach. Targeted metabolomics analysis indicated that the SC-RE treatment induced cholesterol accumulation after 24 h. Transcriptomic analysis suggested that most of the changes induced by the SC-RE and CA were orchestrated by unfolded protein response (UPR) and triggered by endoplasmic reticulum stress. Results suggested up-regulation of VLDLR gene as the principal contributor to the observed cholesterol accumulation in SC-RE-treated cells. In addition, the SC-RE attenuated the activity of E2F transcription factor, down-regulating several genes involved in G1-S transition of the cell cycle.This work was supported by an AGL2011-29857-C03-01 project (Ministerio de Educacion y Ciencia, Spain) and S2013/ABI-2728 (Comunidad de Madrid). A.V. thanks the Ministerio de Economía y Competitividad for his FPI pre-doctoral fellowship (BES-2012-057014).Peer reviewedPeer ReviewedElsevierComunidad de MadridMinisterio de Economía y Competitividad (España)Ministerio de Educación y Ciencia (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/149960reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésInglés#PLACEHOLDER_PARENT_METADATA_VALUE#S2013/ABI-2728/ALIBIRD-CMThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.jff.2015.03.043https://doi.org/10.1016/j.jff.2015.03.043Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1499602026-05-22T06:33:51Z
dc.title.none.fl_str_mv Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells
title Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells
spellingShingle Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells
Valdés, Alberto
Transcriptomics
Colon cancer
Foodomics
Rosemary
Unfolded protein response
Carnosic acid
title_short Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells
title_full Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells
title_fullStr Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells
title_full_unstemmed Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells
title_sort Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in colon cancer cells
dc.creator.none.fl_str_mv Valdés, Alberto
Sullini, Giuseppe
Ibáñez, Elena
Cifuentes, Alejandro
García-Cañas, Virginia
author Valdés, Alberto
author_facet Valdés, Alberto
Sullini, Giuseppe
Ibáñez, Elena
Cifuentes, Alejandro
García-Cañas, Virginia
author_role author
author2 Sullini, Giuseppe
Ibáñez, Elena
Cifuentes, Alejandro
García-Cañas, Virginia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
Ministerio de Economía y Competitividad (España)
Ministerio de Educación y Ciencia (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Transcriptomics
Colon cancer
Foodomics
Rosemary
Unfolded protein response
Carnosic acid
topic Transcriptomics
Colon cancer
Foodomics
Rosemary
Unfolded protein response
Carnosic acid
description Several studies have demonstrated that rosemary polyphenols exert changes in the lipid metabolism in adipose and hepatic cells. In this work, the effects of a polyphenol-enriched supercritical rosemary extract (SC-RE) and carnosic acid (CA) on the transcriptome and cholesterol metabolism in HT-29 colon cancer cells were examined using a Foodomics approach. Targeted metabolomics analysis indicated that the SC-RE treatment induced cholesterol accumulation after 24 h. Transcriptomic analysis suggested that most of the changes induced by the SC-RE and CA were orchestrated by unfolded protein response (UPR) and triggered by endoplasmic reticulum stress. Results suggested up-regulation of VLDLR gene as the principal contributor to the observed cholesterol accumulation in SC-RE-treated cells. In addition, the SC-RE attenuated the activity of E2F transcription factor, down-regulating several genes involved in G1-S transition of the cell cycle.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/149960
url http://hdl.handle.net/10261/149960
dc.language.none.fl_str_mv Inglés
Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
S2013/ABI-2728/ALIBIRD-CM
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.jff.2015.03.043
https://doi.org/10.1016/j.jff.2015.03.043
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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