Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture

Multiple myeloma is a hematologic neoplasm caused by an uncontrolled clonal proliferation of neoplastic plasma cells (nPCs) in the bone marrow. The development and survival of this disease is tightly related to the bone marrow environment. Proliferation and viability of nPCs depend on their interact...

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Authors: Marín Pallá, Juan Carlos, Cordón, Lourdes, Sempere, Amparo, Clara-Trujillo, Sandra|||0000-0002-6455-2993, Gallego-Ferrer, Gloria|||0000-0002-2428-0903, Gómez Ribelles, José Luís|||0000-0001-9099-0885
Format: conjunto de datos
Publication Date:2022
Country:España
Institution:Universitat Politècnica de València (UPV)
Repository:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Language:English
OAI Identifier:oai:riunet.upv.es:10251/189950
Online Access:https://riunet.upv.es/handle/10251/189950
Access Level:Open access
Keyword:Multiple myeloma
Microgels
Fibronectin
Bortezomib
Dexamethasone
CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA
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spelling Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D CultureMarín Pallá, Juan CarlosCordón, LourdesSempere, AmparoClara-Trujillo, Sandra|||0000-0002-6455-2993Gallego-Ferrer, Gloria|||0000-0002-2428-0903Gómez Ribelles, José Luís|||0000-0001-9099-0885Multiple myelomaMicrogelsFibronectinBortezomibDexamethasoneCIENCIA DE LOS MATERIALES E INGENIERIA METALURGICAMultiple myeloma is a hematologic neoplasm caused by an uncontrolled clonal proliferation of neoplastic plasma cells (nPCs) in the bone marrow. The development and survival of this disease is tightly related to the bone marrow environment. Proliferation and viability of nPCs depend on their interaction with the stromal cells and the extracellular matrix components, which also influences the appearance of drug resistance. Recapitulating these interactions in an in vitro culture requires 3D environments that incorporate the biomolecules of interest. In this work, we studied the proliferation and viability of three multiple myeloma cell lines in a microgel consisting of biostable microspheres with fibronectin (FN) on their surfaces. We also showed that the interaction of the RPMI8226 cell line with FN induced cell arrest in the G0/G1 cell cycle phase. RPMI8226 cells developed a significant resistance to dexamethasone, which was reduced when they were treated with dexamethasone and bortezomib in combination.CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011, Iniciativa Ingenio 2010, and Consolider Program. CIBER Actions were financed by the Instituto de Salud Carlos III, with assistance from the European Regional Development Fund. The kind supplying of RPMI 8226 cells by Beatriz Martin (Josep Carreras Leukaemia Research Institute) is greatly acknowledged. The Microscopy Service of the UPV (Universitat Politècnica de València) is gratefully acknowledged.MDPIUniversitat Politècnica de ValènciaDepartamento de Termodinámica AplicadaCentro de Biomateriales e Ingeniería TisularEscuela Técnica Superior de Ingeniería IndustrialInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalAgencia Estatal de InvestigaciónGeneralitat ValencianaRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-11-21datasethttp://purl.org/coar/resource_type/c_ddb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/datasetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheettext/plainhttps://riunet.upv.es/handle/10251/189950reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106099RB-C41 MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLEGeneralitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2016%2F063 MEDULA OSEA ARTIFICIAL PARA PERSONALIZAR EL TRATAMIENTO DE PACIENTES DE CANCERES DE SANGREopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1899502026-06-13T07:49:27Z
dc.title.none.fl_str_mv Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
title Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
spellingShingle Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
Marín Pallá, Juan Carlos
Multiple myeloma
Microgels
Fibronectin
Bortezomib
Dexamethasone
CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA
title_short Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
title_full Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
title_fullStr Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
title_full_unstemmed Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
title_sort Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
dc.creator.none.fl_str_mv Marín Pallá, Juan Carlos
Cordón, Lourdes
Sempere, Amparo
Clara-Trujillo, Sandra|||0000-0002-6455-2993
Gallego-Ferrer, Gloria|||0000-0002-2428-0903
Gómez Ribelles, José Luís|||0000-0001-9099-0885
author Marín Pallá, Juan Carlos
author_facet Marín Pallá, Juan Carlos
Cordón, Lourdes
Sempere, Amparo
Clara-Trujillo, Sandra|||0000-0002-6455-2993
Gallego-Ferrer, Gloria|||0000-0002-2428-0903
Gómez Ribelles, José Luís|||0000-0001-9099-0885
author_role author
author2 Cordón, Lourdes
Sempere, Amparo
Clara-Trujillo, Sandra|||0000-0002-6455-2993
Gallego-Ferrer, Gloria|||0000-0002-2428-0903
Gómez Ribelles, José Luís|||0000-0001-9099-0885
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Termodinámica Aplicada
Centro de Biomateriales e Ingeniería Tisular
Escuela Técnica Superior de Ingeniería Industrial
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Agencia Estatal de Investigación
Generalitat Valenciana
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Multiple myeloma
Microgels
Fibronectin
Bortezomib
Dexamethasone
CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA
topic Multiple myeloma
Microgels
Fibronectin
Bortezomib
Dexamethasone
CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA
description Multiple myeloma is a hematologic neoplasm caused by an uncontrolled clonal proliferation of neoplastic plasma cells (nPCs) in the bone marrow. The development and survival of this disease is tightly related to the bone marrow environment. Proliferation and viability of nPCs depend on their interaction with the stromal cells and the extracellular matrix components, which also influences the appearance of drug resistance. Recapitulating these interactions in an in vitro culture requires 3D environments that incorporate the biomolecules of interest. In this work, we studied the proliferation and viability of three multiple myeloma cell lines in a microgel consisting of biostable microspheres with fibronectin (FN) on their surfaces. We also showed that the interaction of the RPMI8226 cell line with FN induced cell arrest in the G0/G1 cell cycle phase. RPMI8226 cells developed a significant resistance to dexamethasone, which was reduced when they were treated with dexamethasone and bortezomib in combination.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-11-21
dc.type.none.fl_str_mv dataset
http://purl.org/coar/resource_type/c_ddb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/189950
url https://riunet.upv.es/handle/10251/189950
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106099RB-C41 MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLE
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2016%2F063 MEDULA OSEA ARTIFICIAL PARA PERSONALIZAR EL TRATAMIENTO DE PACIENTES DE CANCERES DE SANGRE
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
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application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
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dc.publisher.none.fl_str_mv MDPI
Universitat Politècnica de València
publisher.none.fl_str_mv MDPI
Universitat Politècnica de València
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
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