CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury

Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditio...

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Autores: Pannunzio, Bruno, Amo Aparicio, Jesús, Julián, Camila, López Vales, Rubèn, Peluffo, Hugo, Lago, Natalia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/187251
Acesso em linha:https://hdl.handle.net/2445/187251
Access Level:acceso abierto
Palavra-chave:Macròfags
Regeneració del sistema nerviós
Fagocitosi
Macrophages
Nervous system regeneration
Phagocytosis
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spelling CD200R1 contributes to successful functional reinnervation after a sciatic nerve injuryPannunzio, BrunoAmo Aparicio, JesúsJulián, CamilaLópez Vales, RubènPeluffo, HugoLago, NataliaMacròfagsRegeneració del sistema nerviósFagocitosiMacrophagesNervous system regenerationPhagocytosisActivating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 inWallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/187251Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/cells11111786Cells, 2022, vol. 11, p. 1786https://doi.org/10.3390/cells11111786cc-by (c) Pannunzio, Bruno et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1872512026-05-27T06:46:51Z
dc.title.none.fl_str_mv CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury
title CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury
spellingShingle CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury
Pannunzio, Bruno
Macròfags
Regeneració del sistema nerviós
Fagocitosi
Macrophages
Nervous system regeneration
Phagocytosis
title_short CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury
title_full CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury
title_fullStr CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury
title_full_unstemmed CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury
title_sort CD200R1 contributes to successful functional reinnervation after a sciatic nerve injury
dc.creator.none.fl_str_mv Pannunzio, Bruno
Amo Aparicio, Jesús
Julián, Camila
López Vales, Rubèn
Peluffo, Hugo
Lago, Natalia
author Pannunzio, Bruno
author_facet Pannunzio, Bruno
Amo Aparicio, Jesús
Julián, Camila
López Vales, Rubèn
Peluffo, Hugo
Lago, Natalia
author_role author
author2 Amo Aparicio, Jesús
Julián, Camila
López Vales, Rubèn
Peluffo, Hugo
Lago, Natalia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Macròfags
Regeneració del sistema nerviós
Fagocitosi
Macrophages
Nervous system regeneration
Phagocytosis
topic Macròfags
Regeneració del sistema nerviós
Fagocitosi
Macrophages
Nervous system regeneration
Phagocytosis
description Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 inWallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/187251
url https://hdl.handle.net/2445/187251
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/cells11111786
Cells, 2022, vol. 11, p. 1786
https://doi.org/10.3390/cells11111786
dc.rights.none.fl_str_mv cc-by (c) Pannunzio, Bruno et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Pannunzio, Bruno et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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