Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation

Metabotropic glutamate receptors (mGluR) are present in cells of the nervous system, where they are activated by one of the main neurotransmitters, glutamate. They are also expressed in cells outside the nervous system. We identified and characterized two receptors belonging to group I mGluR, mGlu1R...

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Autores: Pacheco, Rodrigo, Ciruela Alférez, Francisco, Casadó, Vicent, Mallol Montero, Josefa, Gallart, Teresa, Lluís i Biset, Carme, Franco Fernández, Rafael
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176929
Acceso en línea:https://hdl.handle.net/2445/176929
Access Level:acceso abierto
Palabra clave:Àcid glutàmic
Fisiologia
Limfòcits
Glutamic acid
Physiology
Lymphocytes
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spelling Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activationPacheco, RodrigoCiruela Alférez, FranciscoCasadó, VicentMallol Montero, JosefaGallart, TeresaLluís i Biset, CarmeFranco Fernández, RafaelÀcid glutàmicFisiologiaLimfòcitsGlutamic acidPhysiologyLymphocytesMetabotropic glutamate receptors (mGluR) are present in cells of the nervous system, where they are activated by one of the main neurotransmitters, glutamate. They are also expressed in cells outside the nervous system. We identified and characterized two receptors belonging to group I mGluR, mGlu1R and mGlu5R, in human cell lines of lymphoid origin and in resting and activated lymphocytes from human peripheral blood. Both are highly expressed in the human Jurkat T cell line, whereas mGlu5R is expressed only in the human B cell line SKW6.4. In blood lymphocytes, mGlu5R is expressed constitutively, whereas mGlu1R is expressed only upon activation via the T cell receptor-CD3 complex. Group I receptors in the central nervous system are coupled to phospholipase C, whereas in blood lymphocytes, activation of mGlu5R does not trigger this signaling pathway, but instead activates adenylate cyclase. On the other hand, mGlu5R does not mediate ERK1/2 activation, whereas mGlu1R, which is coupled neither to phospholipase C nor to calcium channels and whose activation does not increase cAMP, activates the mitogen-activated protein kinase cascade. The differential expression of mGluR in resting and activated lymphocytes and the different signaling pathways that are triggered when mGlu1Rs or mGlu5Rs are activated point to a key role of glutamate in the regulation of T cell physiological function. The study of the signaling pathways (cAMP production and ERK1/2 phosphorylation) and the proliferative response obtained in the presence of glutamate analogs suggests that mGlu1R and mGlu5R have distinct functions. mGlu5R mediates the reported inhibition of cell proliferation evoked by glutamate, which is reverted by the activation of inducible mGlu1R. This is a novel non-inhibitory action mechanism for glutamate in lymphocyte activation. mGlu1R and mGlu5R thus mediate opposite glutamate effects in human lymphocytes.American Society for Biochemistry and Molecular Biology2021202120042021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfhttps://hdl.handle.net/2445/176929Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1074/jbc.M401761200Journal of Biological Chemistry, 2004, vol. 279, num. 32, p. 33352-33358https://doi.org/10.1074/jbc.M401761200(c) American Society for Biochemistry and Molecular Biology, 2004info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1769292026-05-29T05:05:01Z
dc.title.none.fl_str_mv Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation
title Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation
spellingShingle Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation
Pacheco, Rodrigo
Àcid glutàmic
Fisiologia
Limfòcits
Glutamic acid
Physiology
Lymphocytes
title_short Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation
title_full Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation
title_fullStr Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation
title_full_unstemmed Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation
title_sort Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation
dc.creator.none.fl_str_mv Pacheco, Rodrigo
Ciruela Alférez, Francisco
Casadó, Vicent
Mallol Montero, Josefa
Gallart, Teresa
Lluís i Biset, Carme
Franco Fernández, Rafael
author Pacheco, Rodrigo
author_facet Pacheco, Rodrigo
Ciruela Alférez, Francisco
Casadó, Vicent
Mallol Montero, Josefa
Gallart, Teresa
Lluís i Biset, Carme
Franco Fernández, Rafael
author_role author
author2 Ciruela Alférez, Francisco
Casadó, Vicent
Mallol Montero, Josefa
Gallart, Teresa
Lluís i Biset, Carme
Franco Fernández, Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Àcid glutàmic
Fisiologia
Limfòcits
Glutamic acid
Physiology
Lymphocytes
topic Àcid glutàmic
Fisiologia
Limfòcits
Glutamic acid
Physiology
Lymphocytes
description Metabotropic glutamate receptors (mGluR) are present in cells of the nervous system, where they are activated by one of the main neurotransmitters, glutamate. They are also expressed in cells outside the nervous system. We identified and characterized two receptors belonging to group I mGluR, mGlu1R and mGlu5R, in human cell lines of lymphoid origin and in resting and activated lymphocytes from human peripheral blood. Both are highly expressed in the human Jurkat T cell line, whereas mGlu5R is expressed only in the human B cell line SKW6.4. In blood lymphocytes, mGlu5R is expressed constitutively, whereas mGlu1R is expressed only upon activation via the T cell receptor-CD3 complex. Group I receptors in the central nervous system are coupled to phospholipase C, whereas in blood lymphocytes, activation of mGlu5R does not trigger this signaling pathway, but instead activates adenylate cyclase. On the other hand, mGlu5R does not mediate ERK1/2 activation, whereas mGlu1R, which is coupled neither to phospholipase C nor to calcium channels and whose activation does not increase cAMP, activates the mitogen-activated protein kinase cascade. The differential expression of mGluR in resting and activated lymphocytes and the different signaling pathways that are triggered when mGlu1Rs or mGlu5Rs are activated point to a key role of glutamate in the regulation of T cell physiological function. The study of the signaling pathways (cAMP production and ERK1/2 phosphorylation) and the proliferative response obtained in the presence of glutamate analogs suggests that mGlu1R and mGlu5R have distinct functions. mGlu5R mediates the reported inhibition of cell proliferation evoked by glutamate, which is reverted by the activation of inducible mGlu1R. This is a novel non-inhibitory action mechanism for glutamate in lymphocyte activation. mGlu1R and mGlu5R thus mediate opposite glutamate effects in human lymphocytes.
publishDate 2004
dc.date.none.fl_str_mv 2004
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176929
url https://hdl.handle.net/2445/176929
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1074/jbc.M401761200
Journal of Biological Chemistry, 2004, vol. 279, num. 32, p. 33352-33358
https://doi.org/10.1074/jbc.M401761200
dc.rights.none.fl_str_mv (c) American Society for Biochemistry and Molecular Biology, 2004
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Biochemistry and Molecular Biology, 2004
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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