Rat1p maintains RNA polymerase II CTD phosphorylation balance

In S. cerevisiae, the 5′-3′ exonuclease Rat1p partakes in transcription termination. Although Rat1p-mediated RNA degradation has been suggested to play a role for this activity, the exact mechanisms by which Rat1p helps release RNA polymerase II (RNAPII) from the DNA template are poorly understood....

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Detalles Bibliográficos
Autores: Jimeno González, Silvia, Schmid, Manfred, Malagón Cobos, Francisco, Haaning, Line Lindegaard, Jensen, Torben Heick
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/82624
Acceso en línea:https://hdl.handle.net/11441/82624
https://doi.org/10.1261/rna.041129.113
Access Level:acceso abierto
Palabra clave:RNA polymerase II
CTD phosphorylation
Transcription elongation
Transcription termination
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spelling Rat1p maintains RNA polymerase II CTD phosphorylation balanceJimeno González, SilviaSchmid, ManfredMalagón Cobos, FranciscoHaaning, Line LindegaardJensen, Torben HeickRNA polymerase IICTD phosphorylationTranscription elongationTranscription terminationIn S. cerevisiae, the 5′-3′ exonuclease Rat1p partakes in transcription termination. Although Rat1p-mediated RNA degradation has been suggested to play a role for this activity, the exact mechanisms by which Rat1p helps release RNA polymerase II (RNAPII) from the DNA template are poorly understood. Here we describe a function of Rat1p in regulating phosphorylation levels of the C-terminal domain (CTD) of the largest RNAPII subunit, Rpb1p, during transcription elongation. The rat1-1 mutant exhibits highly elevated levels of CTD phosphorylation as well as RNAPII distribution and transcription termination defects. These phenotypes are all rescued by overexpression of the CTD phosphatase Fcp1p, suggesting a functional relationship between the absence of Rat1p activity, elevated CTD phosphorylation, and transcription defects. We also demonstrate that rat1-1 cells display increased RNAPII transcription kinetics, a feature that may contribute to the cellular phenotypes of the mutant. Consistently, the rat1-1 allele is synthetic lethal with the rpb1-E1103G mutation, causing increased RNAPII speed, and is suppressed by the rpb2-10 mutation, causing slowed transcription. Thus, Rat1p plays more complex roles in controlling transcription than previously thought.Cold Spring Harbor Laboratory PressGenética2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/82624https://doi.org/10.1261/rna.041129.113reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésRNA, 20, 551-558.http://dx.doi.org/10.1261/rna.041129.113info:eu-repo/semantics/openAccessoai:idus.us.es:11441/826242026-06-17T12:51:07Z
dc.title.none.fl_str_mv Rat1p maintains RNA polymerase II CTD phosphorylation balance
title Rat1p maintains RNA polymerase II CTD phosphorylation balance
spellingShingle Rat1p maintains RNA polymerase II CTD phosphorylation balance
Jimeno González, Silvia
RNA polymerase II
CTD phosphorylation
Transcription elongation
Transcription termination
title_short Rat1p maintains RNA polymerase II CTD phosphorylation balance
title_full Rat1p maintains RNA polymerase II CTD phosphorylation balance
title_fullStr Rat1p maintains RNA polymerase II CTD phosphorylation balance
title_full_unstemmed Rat1p maintains RNA polymerase II CTD phosphorylation balance
title_sort Rat1p maintains RNA polymerase II CTD phosphorylation balance
dc.creator.none.fl_str_mv Jimeno González, Silvia
Schmid, Manfred
Malagón Cobos, Francisco
Haaning, Line Lindegaard
Jensen, Torben Heick
author Jimeno González, Silvia
author_facet Jimeno González, Silvia
Schmid, Manfred
Malagón Cobos, Francisco
Haaning, Line Lindegaard
Jensen, Torben Heick
author_role author
author2 Schmid, Manfred
Malagón Cobos, Francisco
Haaning, Line Lindegaard
Jensen, Torben Heick
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Genética
dc.subject.none.fl_str_mv RNA polymerase II
CTD phosphorylation
Transcription elongation
Transcription termination
topic RNA polymerase II
CTD phosphorylation
Transcription elongation
Transcription termination
description In S. cerevisiae, the 5′-3′ exonuclease Rat1p partakes in transcription termination. Although Rat1p-mediated RNA degradation has been suggested to play a role for this activity, the exact mechanisms by which Rat1p helps release RNA polymerase II (RNAPII) from the DNA template are poorly understood. Here we describe a function of Rat1p in regulating phosphorylation levels of the C-terminal domain (CTD) of the largest RNAPII subunit, Rpb1p, during transcription elongation. The rat1-1 mutant exhibits highly elevated levels of CTD phosphorylation as well as RNAPII distribution and transcription termination defects. These phenotypes are all rescued by overexpression of the CTD phosphatase Fcp1p, suggesting a functional relationship between the absence of Rat1p activity, elevated CTD phosphorylation, and transcription defects. We also demonstrate that rat1-1 cells display increased RNAPII transcription kinetics, a feature that may contribute to the cellular phenotypes of the mutant. Consistently, the rat1-1 allele is synthetic lethal with the rpb1-E1103G mutation, causing increased RNAPII speed, and is suppressed by the rpb2-10 mutation, causing slowed transcription. Thus, Rat1p plays more complex roles in controlling transcription than previously thought.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/82624
https://doi.org/10.1261/rna.041129.113
url https://hdl.handle.net/11441/82624
https://doi.org/10.1261/rna.041129.113
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv RNA, 20, 551-558.
http://dx.doi.org/10.1261/rna.041129.113
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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