Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes

Fungal endophytes develop inside plants without visible external signs, and they may confer adaptive advantages to their hosts. Culturing methods have been traditionally used to recognize the fungal endophytic assemblage, but novel metabarcoding techniques are being increasingly applied. This study...

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Autores: Durán Lázaro, María, San Emeterio Garciandía, Leticia, Canals Tresserras, Rosa María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/42673
Acceso en línea:https://hdl.handle.net/2454/42673
Access Level:acceso abierto
Palabra clave:Brachypodium rupestre
Culturing
Fire
Grazing
Metabarcoding
Mycobiome
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spelling Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimesDurán Lázaro, MaríaSan Emeterio Garciandía, LeticiaCanals Tresserras, Rosa MaríaBrachypodium rupestreCulturingFireGrazingMetabarcodingMycobiomeFungal endophytes develop inside plants without visible external signs, and they may confer adaptive advantages to their hosts. Culturing methods have been traditionally used to recognize the fungal endophytic assemblage, but novel metabarcoding techniques are being increasingly applied. This study aims to characterize the fungal endophytic assemblage in shoots, rhizomes and roots of the tall grass Brachypodium rupestre growing in a large area of natural grasslands with a continuum of anthropized disturbance regimes. Seven out of 88 taxa identified via metabarcoding accounted for 81.2% of the reads (Helotiaceae, Lachnum sp. A, Albotricha sp. A, Helotiales A, Agaricales A, Mycena sp. and Mollisiaceae C), revealing a small group of abundant endophytes and a large group of rare species. Although both methods detected the same trends in richness and fungal diversity among the tissues (root > rhizome > shoot) and grasslands (low-diversity >high-diversity grasslands), the metabarcoding tool identified 5.8 times more taxa than the traditional culturing method (15 taxa) but, surprisingly, failed to sequence the most isolated endophyte on plates, Omnidemptus graminis. Since both methods are still subject to important constraints, both are required to obtain a complete characterization of the fungal endophytic assemblage of the plant species.M.D. got funding through UPNA’s doctorate scholarship. This research was supported by 'la Caixa' Foundation and CAN foundation (LCF/PR/PR13/51080004), Ecoembes-SEO/Birdlife (Libera 2017), Government of Navarra (STEM research project 2018) and Interreg Sudoe Programme, European Regional Development Fund, European-Union, Open2preserve Project (SOE2/P5/E0804).MDPIAgronomia, Bioteknologia eta ElikaduraInstitute on Innovation and Sustainable Development in Food Chain - ISFOODAgronomía, Biotecnología y AlimentaciónGobierno de Navarra / Nafarroako Gobernua, CENEDUCA18Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/42673reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/426732026-06-17T12:41:47Z
dc.title.none.fl_str_mv Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes
title Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes
spellingShingle Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes
Durán Lázaro, María
Brachypodium rupestre
Culturing
Fire
Grazing
Metabarcoding
Mycobiome
title_short Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes
title_full Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes
title_fullStr Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes
title_full_unstemmed Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes
title_sort Comparison of culturing and metabarcoding methods to describe the fungal endophytic assemblage of brachypodium rupestre growing in a range of anthropized disturbance regimes
dc.creator.none.fl_str_mv Durán Lázaro, María
San Emeterio Garciandía, Leticia
Canals Tresserras, Rosa María
author Durán Lázaro, María
author_facet Durán Lázaro, María
San Emeterio Garciandía, Leticia
Canals Tresserras, Rosa María
author_role author
author2 San Emeterio Garciandía, Leticia
Canals Tresserras, Rosa María
author2_role author
author
dc.contributor.none.fl_str_mv Agronomia, Bioteknologia eta Elikadura
Institute on Innovation and Sustainable Development in Food Chain - ISFOOD
Agronomía, Biotecnología y Alimentación
Gobierno de Navarra / Nafarroako Gobernua, CENEDUCA18
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
dc.subject.none.fl_str_mv Brachypodium rupestre
Culturing
Fire
Grazing
Metabarcoding
Mycobiome
topic Brachypodium rupestre
Culturing
Fire
Grazing
Metabarcoding
Mycobiome
description Fungal endophytes develop inside plants without visible external signs, and they may confer adaptive advantages to their hosts. Culturing methods have been traditionally used to recognize the fungal endophytic assemblage, but novel metabarcoding techniques are being increasingly applied. This study aims to characterize the fungal endophytic assemblage in shoots, rhizomes and roots of the tall grass Brachypodium rupestre growing in a large area of natural grasslands with a continuum of anthropized disturbance regimes. Seven out of 88 taxa identified via metabarcoding accounted for 81.2% of the reads (Helotiaceae, Lachnum sp. A, Albotricha sp. A, Helotiales A, Agaricales A, Mycena sp. and Mollisiaceae C), revealing a small group of abundant endophytes and a large group of rare species. Although both methods detected the same trends in richness and fungal diversity among the tissues (root > rhizome > shoot) and grasslands (low-diversity >high-diversity grasslands), the metabarcoding tool identified 5.8 times more taxa than the traditional culturing method (15 taxa) but, surprisingly, failed to sequence the most isolated endophyte on plates, Omnidemptus graminis. Since both methods are still subject to important constraints, both are required to obtain a complete characterization of the fungal endophytic assemblage of the plant species.
publishDate 2021
dc.date.none.fl_str_mv 2021
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dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/42673
url https://hdl.handle.net/2454/42673
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv MDPI
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instname_str Universidad Pública de Navarra
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