A novel methodology to characterize beat-to-beat alternations in whole-cell calcium currents
An automated high-throughput procedure to quantify the degree of electrical beat-to- beat alternants in human atrial myocytes from electrophysiological recordings of the transmembrane ion currents of calcium ions (Ca2+) is presented and discussed. The patch clamp technique in whole-cell mode was use...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/456384 |
| Acceso en línea: | https://hdl.handle.net/2117/456384 https://dx.doi.org/10.1371/journal.pone.0339890 |
| Access Level: | acceso abierto |
| Palabra clave: | Àrees temàtiques de la UPC::Enginyeria biomèdica |
| Sumario: | An automated high-throughput procedure to quantify the degree of electrical beat-to- beat alternants in human atrial myocytes from electrophysiological recordings of the transmembrane ion currents of calcium ions (Ca2+) is presented and discussed. The patch clamp technique in whole-cell mode was used to record the myocyte calcium signal. A database consisting of 24 patch clamp signals (N = 24) of which 13 had a uniform behaviour and 11 had an alternating behaviour, was created. Several fea- tures were computed to characterize the transmembrane ion currents: peak ampli- tude, time constants, and area under the ion current trace. The presented algorithm includes a feature detector whose accuracy has been validated using simulated calcium signals generated by an electrical model that accurately represents the cardiomyocyte behaviour in a patch clamp experiment. Among these calculated fea- tures, a new index measure, called the “alternation index”, is proposed in this work to quantify the degree of electrical beat-to-beat alternants. The index has been shown to be a robust measure (p = 0.01 **) for cell detection with an alternating pattern. Good agreement was observed with alternations in other calculated features like the measurement of the inactivation of L-type calcium current (Ica) or the tail current (ITail) generated by calcium extrusion upon repolarization. |
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