Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy

The elastic response of a living cell is affected by its physiological state. This property provides mechanical fingerprints of a cell's dysfunctionality. The softness (kilopascal range) and thickness (2–15 μm) of mammalian cells imply that the force exerted by the probe might be affected by th...

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Detalles Bibliográficos
Autores: García, Pablo D., García García, Ricardo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/183082
Acceso en línea:http://hdl.handle.net/10261/183082
Access Level:acceso abierto
Palabra clave:Contact mechanics
Force spectroscopy
Elastic properties
Cell nanomechanics
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spelling Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force MicroscopyGarcía, Pablo D.García García, RicardoContact mechanicsForce spectroscopyElastic propertiesCell nanomechanicsThe elastic response of a living cell is affected by its physiological state. This property provides mechanical fingerprints of a cell's dysfunctionality. The softness (kilopascal range) and thickness (2–15 μm) of mammalian cells imply that the force exerted by the probe might be affected by the stiffness of the solid support. This observation makes infinite sample thickness models unsuitable to describe quantitatively the forces and deformations on a cell. Here, we report a general theory to determine the true Young's moduli of a single cell from a force-indentation curve. Analytical expressions are deduced for common geometries such as flat punches, paraboloids, cones, needles, and nanowires. For a given cell and indentation, the influence of the solid support on the measurements is reduced by using sharp and high aspect ratio tips. The theory is validated by finite element simulations.We acknowledge financial support from the European Research Council ERC–AdG–340177 (3DNanoMech) and the Ministerio de Economía y Competitividad MAT2016-76507-RPeer ReviewedBiophysical SocietyMinisterio de Economía y Competitividad (España)European Research CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920182019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/183082reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2016-76507-RERC–AdG–340177https://doi.org/10.1016/j.bpj.2018.05.012Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1830822026-05-22T06:33:51Z
dc.title.none.fl_str_mv Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy
title Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy
spellingShingle Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy
García, Pablo D.
Contact mechanics
Force spectroscopy
Elastic properties
Cell nanomechanics
title_short Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy
title_full Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy
title_fullStr Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy
title_full_unstemmed Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy
title_sort Determination of the Elastic Moduli of a Single Cell Cultured on a Rigid Support by Force Microscopy
dc.creator.none.fl_str_mv García, Pablo D.
García García, Ricardo
author García, Pablo D.
author_facet García, Pablo D.
García García, Ricardo
author_role author
author2 García García, Ricardo
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Research Council
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Contact mechanics
Force spectroscopy
Elastic properties
Cell nanomechanics
topic Contact mechanics
Force spectroscopy
Elastic properties
Cell nanomechanics
description The elastic response of a living cell is affected by its physiological state. This property provides mechanical fingerprints of a cell's dysfunctionality. The softness (kilopascal range) and thickness (2–15 μm) of mammalian cells imply that the force exerted by the probe might be affected by the stiffness of the solid support. This observation makes infinite sample thickness models unsuitable to describe quantitatively the forces and deformations on a cell. Here, we report a general theory to determine the true Young's moduli of a single cell from a force-indentation curve. Analytical expressions are deduced for common geometries such as flat punches, paraboloids, cones, needles, and nanowires. For a given cell and indentation, the influence of the solid support on the measurements is reduced by using sharp and high aspect ratio tips. The theory is validated by finite element simulations.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/183082
url http://hdl.handle.net/10261/183082
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2016-76507-R
ERC–AdG–340177
https://doi.org/10.1016/j.bpj.2018.05.012
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Biophysical Society
publisher.none.fl_str_mv Biophysical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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