Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?

Double-strand breaks (DSBs) are the most harmful DNA lesions, with a strong impact on cell proliferation and genome integrity. Depending on cell cycle stage, DSBs are preferentially repaired by non-homologous end joining or homologous recombination (HR). In recent years, numerous reports have reveal...

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Detalles Bibliográficos
Autores: Gómez-González, Belén, Aguilera, Andrés
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/346574
Acceso en línea:http://hdl.handle.net/10261/346574
Access Level:acceso abierto
Palabra clave:DNA damage
DNA–RNA hybrids
DSBs
Recombination
Repair
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spelling Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?Gómez-González, BelénAguilera, AndrésDNA damageDNA–RNA hybridsDSBsRecombinationRepairDouble-strand breaks (DSBs) are the most harmful DNA lesions, with a strong impact on cell proliferation and genome integrity. Depending on cell cycle stage, DSBs are preferentially repaired by non-homologous end joining or homologous recombination (HR). In recent years, numerous reports have revealed that DSBs enhance DNA–RNA hybrid formation around the break site. We call these hybrids “break-induced RNA–DNA hybrids” (BIRDHs) to differentiate them from sporadic R-loops consisting of DNA–RNA hybrids and a displaced single-strand DNA occurring co-transcriptionally in intact DNA. Here, we review and discuss the most relevant data about BIRDHs, with a focus on two main questions raised: (i) whether BIRDHs form by de novo transcription after a DSB or by a pre-existing nascent RNA in DNA regions undergoing transcription and (ii) whether they have a positive role in HR or are just obstacles to HR accidentally generated as an intrinsic risk of transcription. We aim to provide a comprehensive view of the exciting and yet unresolved questions about the source and impact of BIRDHs in the cell.Research in A.A.'s lab is funded by the Agencia Estatal de Investigación (AEI) of the Spanish Ministry of Science, the Junta de Andalucía, Foundation Vencer el Cáncer and the European regional funds FEDER.Peer reviewedEMBO PressAgencia Estatal de Investigación (España)Junta de AndalucíaFundación Vencer el CancerEuropean CommissionMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420232024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_dcae04bcPublisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/346574reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésInglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at http://dx.doi.org/10.15252/embr.202357801http://dx.doi.org/10.15252/embr.202357801Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3465742026-05-22T06:33:51Z
dc.title.none.fl_str_mv Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
title Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
spellingShingle Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
Gómez-González, Belén
DNA damage
DNA–RNA hybrids
DSBs
Recombination
Repair
title_short Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
title_full Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
title_fullStr Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
title_full_unstemmed Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
title_sort Break-induced RNA–DNA hybrids (BIRDHs) in homologous recombination: friend or foe?
dc.creator.none.fl_str_mv Gómez-González, Belén
Aguilera, Andrés
author Gómez-González, Belén
author_facet Gómez-González, Belén
Aguilera, Andrés
author_role author
author2 Aguilera, Andrés
author2_role author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Junta de Andalucía
Fundación Vencer el Cancer
European Commission
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv DNA damage
DNA–RNA hybrids
DSBs
Recombination
Repair
topic DNA damage
DNA–RNA hybrids
DSBs
Recombination
Repair
description Double-strand breaks (DSBs) are the most harmful DNA lesions, with a strong impact on cell proliferation and genome integrity. Depending on cell cycle stage, DSBs are preferentially repaired by non-homologous end joining or homologous recombination (HR). In recent years, numerous reports have revealed that DSBs enhance DNA–RNA hybrid formation around the break site. We call these hybrids “break-induced RNA–DNA hybrids” (BIRDHs) to differentiate them from sporadic R-loops consisting of DNA–RNA hybrids and a displaced single-strand DNA occurring co-transcriptionally in intact DNA. Here, we review and discuss the most relevant data about BIRDHs, with a focus on two main questions raised: (i) whether BIRDHs form by de novo transcription after a DSB or by a pre-existing nascent RNA in DNA regions undergoing transcription and (ii) whether they have a positive role in HR or are just obstacles to HR accidentally generated as an intrinsic risk of transcription. We aim to provide a comprehensive view of the exciting and yet unresolved questions about the source and impact of BIRDHs in the cell.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_dcae04bc
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/346574
url http://hdl.handle.net/10261/346574
dc.language.none.fl_str_mv Inglés
Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at http://dx.doi.org/10.15252/embr.202357801
http://dx.doi.org/10.15252/embr.202357801
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EMBO Press
publisher.none.fl_str_mv EMBO Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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