Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting

Metagenomic and transcriptomic techniques applied to composting could increase our understanding of the overall microbial ecology and could help us to optimise operational conditions which are directly related with economic interest. In this study, the fungal diversity and abundance of two-phase oli...

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Autores: Tortosa Muñoz, Germán, Torralbo, F., Maza-Márquez, Paula, Aranda, Elisabet, Calvo, Carlos, González-Murua, Carmen, Bedmar, Eulogio J.
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/202003
Acceso en línea:http://hdl.handle.net/10261/202003
Access Level:acceso abierto
Palabra clave:Fungal diversity
Illumina MiSeq
Two-phase olive mill waste
qPCR
cDNA
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spelling Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) compostingTortosa Muñoz, GermánTorralbo, F.Maza-Márquez, PaulaAranda, ElisabetCalvo, CarlosGonzález-Murua, CarmenBedmar, Eulogio J.Fungal diversityIllumina MiSeqTwo-phase olive mill wasteqPCRcDNAMetagenomic and transcriptomic techniques applied to composting could increase our understanding of the overall microbial ecology and could help us to optimise operational conditions which are directly related with economic interest. In this study, the fungal diversity and abundance of two-phase olive mill waste (“alperujo”) composting was studied using Illumina MiSeq sequencing and quantitative PCR, respectively. The results showed an increase of the fungal diversity during the process, with Ascomycota being the predominant phylum. Penicillium was the main genera identified at the mesophilic and maturation phases, with Debaryomyces and Sarocladium at the thermophilic phase, respectively. The fungal abundance was increased during composting, which confirms their important role during thermophilic and maturation phases. Some Basidiomycota showed an increased during the process, which showed a positive correlation with the humification parameters. According to that, the genus Cystofilobasidium could be used as a potential fungal biomarker to assess alperujo compost maturation.This work was supported by the ERDF-co-financed projects from Junta de Andalucía (Spain) [P12-AGR-1968], the Spanish MINECO [AGL2015-64582-CO3-02] and MINECO-CSIC RECUPERA 2020 [20134R070]. G. Tortosa thanks the farmer J. González Almendros for his financial and technical assistance during AL composting and also, A. J. Fernández-González, A. Vicente-Lasa, M. Fernández-López and P. J. Villadas for their helpful discussions. Support by Basque Country Government [IT-583 932-16] and the Spanish Red Sirena [AGL2015-68881-REDT] are also acknowledged. E. Aranda thanks MINECO-ERDF for supporting her Ramón y Cajal project [RYC-2013-12481] and [CTM2017-84332-R].ElsevierJunta de AndalucíaMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_816binfo:eu-repo/semantics/preprinthttp://hdl.handle.net/10261/202003reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64582-C3-2-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-68881-REDThttp://dx.doi.org/10.1016/j.biortech.2019.122267Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2020032026-05-22T06:33:51Z
dc.title.none.fl_str_mv Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
title Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
spellingShingle Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
Tortosa Muñoz, Germán
Fungal diversity
Illumina MiSeq
Two-phase olive mill waste
qPCR
cDNA
title_short Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
title_full Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
title_fullStr Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
title_full_unstemmed Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
title_sort Assessment of the diversity and abundance of the total and active fungal population and its correlation with humification during two-phase olive mill waste (‘‘alperujo”) composting
dc.creator.none.fl_str_mv Tortosa Muñoz, Germán
Torralbo, F.
Maza-Márquez, Paula
Aranda, Elisabet
Calvo, Carlos
González-Murua, Carmen
Bedmar, Eulogio J.
author Tortosa Muñoz, Germán
author_facet Tortosa Muñoz, Germán
Torralbo, F.
Maza-Márquez, Paula
Aranda, Elisabet
Calvo, Carlos
González-Murua, Carmen
Bedmar, Eulogio J.
author_role author
author2 Torralbo, F.
Maza-Márquez, Paula
Aranda, Elisabet
Calvo, Carlos
González-Murua, Carmen
Bedmar, Eulogio J.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fungal diversity
Illumina MiSeq
Two-phase olive mill waste
qPCR
cDNA
topic Fungal diversity
Illumina MiSeq
Two-phase olive mill waste
qPCR
cDNA
description Metagenomic and transcriptomic techniques applied to composting could increase our understanding of the overall microbial ecology and could help us to optimise operational conditions which are directly related with economic interest. In this study, the fungal diversity and abundance of two-phase olive mill waste (“alperujo”) composting was studied using Illumina MiSeq sequencing and quantitative PCR, respectively. The results showed an increase of the fungal diversity during the process, with Ascomycota being the predominant phylum. Penicillium was the main genera identified at the mesophilic and maturation phases, with Debaryomyces and Sarocladium at the thermophilic phase, respectively. The fungal abundance was increased during composting, which confirms their important role during thermophilic and maturation phases. Some Basidiomycota showed an increased during the process, which showed a positive correlation with the humification parameters. According to that, the genus Cystofilobasidium could be used as a potential fungal biomarker to assess alperujo compost maturation.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_816b
dc.type.openaire.fl_str_mv info:eu-repo/semantics/preprint
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/202003
url http://hdl.handle.net/10261/202003
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64582-C3-2-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-68881-REDT
http://dx.doi.org/10.1016/j.biortech.2019.122267
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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