Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus

Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), contai...

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Detalles Bibliográficos
Autores: Gómez, Nestor, Wigdorovitz, Andres, Castañón, Sonia, Gil Dones, Félix, Ordá, Ricardo, Borca, Manuel, Escribano, José
Tipo de recurso: artículo
Fecha de publicación:2000
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/94970
Acceso en línea:https://hdl.handle.net/20.500.14352/94970
Access Level:acceso abierto
Palabra clave:581.15
577.112
Gastroenteritis coronavirus
Spikeprotein
Botánica (Biología)
Genética
2417 Biología Vegetal (Botánica)
2409.92 Genética Molecular de Plantas
id ES_09e85579ca66b82e75bebb175e81f3fc
oai_identifier_str oai:docta.ucm.es:20.500.14352/94970
network_acronym_str ES
network_name_str España
repository_id_str
spelling Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirusGómez, NestorWigdorovitz, AndresCastañón, SoniaGil Dones, FélixOrdá, RicardoBorca, ManuelEscribano, José581.15577.112Gastroenteritis coronavirusSpikeproteinBotánica (Biología)Genética2417 Biología Vegetal (Botánica)2409.92 Genética Molecular de PlantasTransgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV.SpringerUniversidad Complutense de Madrid20002000-01-0120002000-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/94970reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/949702026-06-02T12:44:21Z
dc.title.none.fl_str_mv Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
spellingShingle Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
Gómez, Nestor
581.15
577.112
Gastroenteritis coronavirus
Spikeprotein
Botánica (Biología)
Genética
2417 Biología Vegetal (Botánica)
2409.92 Genética Molecular de Plantas
title_short Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_full Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_fullStr Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_full_unstemmed Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_sort Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
dc.creator.none.fl_str_mv Gómez, Nestor
Wigdorovitz, Andres
Castañón, Sonia
Gil Dones, Félix
Ordá, Ricardo
Borca, Manuel
Escribano, José
author Gómez, Nestor
author_facet Gómez, Nestor
Wigdorovitz, Andres
Castañón, Sonia
Gil Dones, Félix
Ordá, Ricardo
Borca, Manuel
Escribano, José
author_role author
author2 Wigdorovitz, Andres
Castañón, Sonia
Gil Dones, Félix
Ordá, Ricardo
Borca, Manuel
Escribano, José
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 581.15
577.112
Gastroenteritis coronavirus
Spikeprotein
Botánica (Biología)
Genética
2417 Biología Vegetal (Botánica)
2409.92 Genética Molecular de Plantas
topic 581.15
577.112
Gastroenteritis coronavirus
Spikeprotein
Botánica (Biología)
Genética
2417 Biología Vegetal (Botánica)
2409.92 Genética Molecular de Plantas
description Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV.
publishDate 2000
dc.date.none.fl_str_mv 2000
2000-01-01
2000
2000-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/94970
url https://hdl.handle.net/20.500.14352/94970
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403133240672256
score 15.81155

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