Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites

In the mouse embryo, the generation of candidate progenitors for long-lasting hemopoiesis has been reported in the paraaortic splanchnopleura (P-Sp)/ aorta-gonad-mesonephros (AGM) region. Here, we address the following question: can the P-Sp/AGM environment support hemopoietic differentiation as wel...

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Detalles Bibliográficos
Autores: Godin, Isabelle, García-Porrero Pérez, Juan Antonio, Dieterlen-Lièvre, Françoise, Cumano, Ana
Tipo de recurso: artículo
Fecha de publicación:1999
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/1658
Acceso en línea:http://hdl.handle.net/10902/1658
Access Level:acceso abierto
Palabra clave:Aorta-gonad-mesonephros
Spleen
Omentum
Hemopoiesis
Reconstitution
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spelling Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sitesGodin, IsabelleGarcía-Porrero Pérez, Juan AntonioDieterlen-Lièvre, FrançoiseCumano, AnaAorta-gonad-mesonephrosSpleenOmentumHemopoiesisReconstitutionIn the mouse embryo, the generation of candidate progenitors for long-lasting hemopoiesis has been reported in the paraaortic splanchnopleura (P-Sp)/ aorta-gonad-mesonephros (AGM) region. Here, we address the following question: can the P-Sp/AGM environment support hemopoietic differentiation as well as generate stem cells, and, conversely, are other sites where hemopoietic differentiation occurs capable of generating stem cells? Although P-Sp/AGM generates de novo hemopoietic stem cells between 9.5 and 12.5 days post coitus (dpc), we show here that it does not support hemopoietic differentiation. Among mesoderm-derived sites, spleen and omentum were shown to be colonized by exogenous cells in the same fashion as the fetal liver. Cells colonizing the spleen were multipotent and pursued their evolution to committed progenitors in this organ. In contrast, the omentum, which was colonized by lymphoid-committed progenitors that did not expand, cannot be considered as a hemopoietic organ. From these data, stem cell generation appears incompatible with hemopoietic activity. At the peak of hemopoietic progenitor production in the P-Sp/AGM, between 10.5 and 11.5 dpc, multipotent cells were found at the exceptional frequency of 1 out of 12 total cells and 1 out of 4 AA4.1+ cells. Thus, progenitors within this region constitute a pool of undifferentiated hemopoietic cells readily accessible for characterization.Rockefeller University PressUniversidad de Cantabria19991999-07-05journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/1658Journal of Experimental Medicine. 1999 Jul 5;190(1):43-52reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-CompartirIgual 3.0 Españahttp://creativecommons.org/licenses/by-nc-sa/3.0/es/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/16582026-06-02T12:39:31Z
dc.title.none.fl_str_mv Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites
title Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites
spellingShingle Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites
Godin, Isabelle
Aorta-gonad-mesonephros
Spleen
Omentum
Hemopoiesis
Reconstitution
title_short Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites
title_full Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites
title_fullStr Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites
title_full_unstemmed Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites
title_sort Stem cell emergence and hemopoietic activity are incompatible in mouse intraembryonic sites
dc.creator.none.fl_str_mv Godin, Isabelle
García-Porrero Pérez, Juan Antonio
Dieterlen-Lièvre, Françoise
Cumano, Ana
author Godin, Isabelle
author_facet Godin, Isabelle
García-Porrero Pérez, Juan Antonio
Dieterlen-Lièvre, Françoise
Cumano, Ana
author_role author
author2 García-Porrero Pérez, Juan Antonio
Dieterlen-Lièvre, Françoise
Cumano, Ana
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
dc.subject.none.fl_str_mv Aorta-gonad-mesonephros
Spleen
Omentum
Hemopoiesis
Reconstitution
topic Aorta-gonad-mesonephros
Spleen
Omentum
Hemopoiesis
Reconstitution
description In the mouse embryo, the generation of candidate progenitors for long-lasting hemopoiesis has been reported in the paraaortic splanchnopleura (P-Sp)/ aorta-gonad-mesonephros (AGM) region. Here, we address the following question: can the P-Sp/AGM environment support hemopoietic differentiation as well as generate stem cells, and, conversely, are other sites where hemopoietic differentiation occurs capable of generating stem cells? Although P-Sp/AGM generates de novo hemopoietic stem cells between 9.5 and 12.5 days post coitus (dpc), we show here that it does not support hemopoietic differentiation. Among mesoderm-derived sites, spleen and omentum were shown to be colonized by exogenous cells in the same fashion as the fetal liver. Cells colonizing the spleen were multipotent and pursued their evolution to committed progenitors in this organ. In contrast, the omentum, which was colonized by lymphoid-committed progenitors that did not expand, cannot be considered as a hemopoietic organ. From these data, stem cell generation appears incompatible with hemopoietic activity. At the peak of hemopoietic progenitor production in the P-Sp/AGM, between 10.5 and 11.5 dpc, multipotent cells were found at the exceptional frequency of 1 out of 12 total cells and 1 out of 4 AA4.1+ cells. Thus, progenitors within this region constitute a pool of undifferentiated hemopoietic cells readily accessible for characterization.
publishDate 1999
dc.date.none.fl_str_mv 1999
1999-07-05
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10902/1658
url http://hdl.handle.net/10902/1658
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 3.0 España
http://creativecommons.org/licenses/by-nc-sa/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-CompartirIgual 3.0 España
http://creativecommons.org/licenses/by-nc-sa/3.0/es/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Rockefeller University Press
publisher.none.fl_str_mv Rockefeller University Press
dc.source.none.fl_str_mv Journal of Experimental Medicine. 1999 Jul 5;190(1):43-52
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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