Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families

16 pages, 7 figures, 2 tables .-- This article is part of the Research Topic Glycobiology and Glycosylation: Deciphering the Secrets of Glycans in Humans and Pathogens

Detalhes bibliográficos
Autores: Campanero-Rhodes, María Asunción, Martí, Sara, Hernández-Ortiz, Noelia, Cubero, Meritxell, Ereño-Orbea, June, Ardá, Ana, Jiménez-Barbero, Jesús, Ardanuy, Carmen, Solís, Dolores
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/374720
Acesso em linha:http://hdl.handle.net/10261/374720
https://api.elsevier.com/content/abstract/scopus_id/85201265500
Access Level:acceso abierto
Palavra-chave:Klebsiella pneumoniae
Siglecs
Bacterial cell surface carbohydrates
Galectins
Hypermucoviscosity
Lectins
id ES_095352fdf2b0b4ac47d2978d1a0e1fb2
oai_identifier_str oai:digital.csic.es:10261/374720
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families
title Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families
spellingShingle Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families
Campanero-Rhodes, María Asunción
Klebsiella pneumoniae
Siglecs
Bacterial cell surface carbohydrates
Galectins
Hypermucoviscosity
Lectins
title_short Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families
title_full Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families
title_fullStr Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families
title_full_unstemmed Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families
title_sort Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families
dc.creator.none.fl_str_mv Campanero-Rhodes, María Asunción
Martí, Sara
Hernández-Ortiz, Noelia
Cubero, Meritxell
Ereño-Orbea, June
Ardá, Ana
Jiménez-Barbero, Jesús
Ardanuy, Carmen
Solís, Dolores
author Campanero-Rhodes, María Asunción
author_facet Campanero-Rhodes, María Asunción
Martí, Sara
Hernández-Ortiz, Noelia
Cubero, Meritxell
Ereño-Orbea, June
Ardá, Ana
Jiménez-Barbero, Jesús
Ardanuy, Carmen
Solís, Dolores
author_role author
author2 Martí, Sara
Hernández-Ortiz, Noelia
Cubero, Meritxell
Ereño-Orbea, June
Ardá, Ana
Jiménez-Barbero, Jesús
Ardanuy, Carmen
Solís, Dolores
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv #NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Klebsiella pneumoniae
Siglecs
Bacterial cell surface carbohydrates
Galectins
Hypermucoviscosity
Lectins
topic Klebsiella pneumoniae
Siglecs
Bacterial cell surface carbohydrates
Galectins
Hypermucoviscosity
Lectins
description 16 pages, 7 figures, 2 tables .-- This article is part of the Research Topic Glycobiology and Glycosylation: Deciphering the Secrets of Glycans in Humans and Pathogens
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/374720
https://api.elsevier.com/content/abstract/scopus_id/85201265500
url http://hdl.handle.net/10261/374720
https://api.elsevier.com/content/abstract/scopus_id/85201265500
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099985-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-143249OB-I00
Frontiers in immunology
https://doi.org/10.3389/fimmu.2024.1436039
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403099395784704
spelling Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin familiesCampanero-Rhodes, María AsunciónMartí, SaraHernández-Ortiz, NoeliaCubero, MeritxellEreño-Orbea, JuneArdá, AnaJiménez-Barbero, JesúsArdanuy, CarmenSolís, DoloresKlebsiella pneumoniaeSiglecsBacterial cell surface carbohydratesGalectinsHypermucoviscosityLectins16 pages, 7 figures, 2 tables .-- This article is part of the Research Topic Glycobiology and Glycosylation: Deciphering the Secrets of Glycans in Humans and PathogensKlebsiella pneumoniae is an opportunistic bacterium that frequently colonizes the nasopharynx and gastrointestinal tract and can also cause severe infections when invading other tissues, particularly in immunocompromised individuals. Moreover, K. pneumoniae variants exhibiting a hypermucoviscous (HMV) phenotype are usually associated with hypervirulent strains that can produce invasive infections even in immunocompetent individuals. Major carbohydrate structures displayed on the K. pneumoniae surface are the polysaccharide capsule and the lipopolysaccharide, which presents an O-polysaccharide chain in its outermost part. Various capsular and O-chain structures have been described. Of note, production of a thick capsule is frequently observed in HMV variants. Here we examined the surface sugar epitopes of a collection of HMV and non-HMV K. pneumoniae clinical isolates and their recognition by several Siglecs and galectins, two lectin families of the innate immune system, using bacteria microarrays as main tool. No significant differences among isolates in sialic acid content or recognition by Siglecs were observed. In contrast, analysis of the binding of model lectins with diverse carbohydrate-binding specificities revealed striking differences in the recognition by galactose- and mannose-specific lectins, which correlated with the binding or lack of binding of galectins and pointed to the O-chain as the plausible ligand. Fluorescence microscopy and microarray analyses of galectin-9 binding to entire cells and outer membranes of two representative HMV isolates supported the bacteria microarray results. In addition, Western blot analysis of the binding of galectin-9 to outer membranes unveiled protein bands recognized by this galectin, and fingerprint analysis of these bands identified several proteins containing potential O-glycosylation sites, thus broadening the spectrum of possible galectin ligands on the K. pneumoniae surface. Moreover, Siglecs and galectins apparently target different structures on K. pneumoniae surfaces, thereby behaving as non-redundant complementary tools of the innate immune system.The author(s) declare financial support was received for the research, author ship, and/or publication of this article. This research was funded by grants RTI2018-099985-B-I00 and PID2022-143249OB-I00 of MCIN/AEI/10.13039/501100011033 and “ERDF A way of making Europe”, and by CIBER (Consorcio Centro de Investigacion Biomedica en Red (CIBERES), Instituto de Salud Carlos III), CB06/06/0037 and CB06/06/1102.Peer reviewedFrontiers Media#NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA##NODATA#Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/374720https://api.elsevier.com/content/abstract/scopus_id/85201265500reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099985-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-143249OB-I00Frontiers in immunologyhttps://doi.org/10.3389/fimmu.2024.1436039Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3747202026-05-22T06:33:51Z
score 15,812429

Registos relacionados