Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase

Cyclodextrin glycosyltransferase (CGTase) has been used to produce cyclodextrins (CDs) from starches, but their ability to modify starches has been barely explored. The effect of CGTase on corn starch at sub-gelatinization temperature (50 °C) and at different pH conditions, pH 4.0 and pH 6.0, was ev...

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Detalles Bibliográficos
Autores: Durá, Ángela, Rosell, Cristina M.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/132245
Acceso en línea:http://hdl.handle.net/10261/132245
Access Level:acceso abierto
Palabra clave:Starch
Enzymatic modification
Pasting properties
Cyclodextrin
Glycosyltransferase
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spelling Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferaseDurá, ÁngelaRosell, Cristina M.StarchEnzymatic modificationPasting propertiesCyclodextrinGlycosyltransferaseCyclodextrin glycosyltransferase (CGTase) has been used to produce cyclodextrins (CDs) from starches, but their ability to modify starches has been barely explored. The effect of CGTase on corn starch at sub-gelatinization temperature (50 °C) and at different pH conditions, pH 4.0 and pH 6.0, was evaluated. Biochemical features, thermal and structural analysis, oligosaccharides and CDs content were studied. Microscopic analysis of the granules confirmed the enzymatic modification of the starches obtaining structures with irregular surface and small pinholes. The extent of the starch modification was largely dependent on the pHs, being higher at pH 6.0. This was also confirmed by the low viscosity of the resulting pastes during a heating and cooling cycle. Thermal parameters were not affected due to enzymatic treatment. Modified starches were less susceptible to undergo α-amylase hydrolysis. CDs released were higher for samples treated at pH 4.0. Therefore, CGTase modification of corn starches at sub-gelatinization temperature offers an attractive alternative for obtaining porous starches with different properties depending on the pH conditions.Financial support of the Spanish Ministry of Economy and Competitiveness (Project AGL2011-23802, AGL2014-52928-C2-1-R), the European Regional Development Fund (FEDER) and Generalitat Valenciana (Project Prometeo 2012/064). A. Dura would like to thank predoctoral fellowship from Spanish Ministry of Economy and Competitiveness.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Generalitat ValencianaEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/132245reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.ijbiomac.2016.03.012Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1322452026-05-22T06:33:51Z
dc.title.none.fl_str_mv Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase
title Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase
spellingShingle Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase
Durá, Ángela
Starch
Enzymatic modification
Pasting properties
Cyclodextrin
Glycosyltransferase
title_short Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase
title_full Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase
title_fullStr Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase
title_full_unstemmed Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase
title_sort Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase
dc.creator.none.fl_str_mv Durá, Ángela
Rosell, Cristina M.
author Durá, Ángela
author_facet Durá, Ángela
Rosell, Cristina M.
author_role author
author2 Rosell, Cristina M.
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Generalitat Valenciana
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Starch
Enzymatic modification
Pasting properties
Cyclodextrin
Glycosyltransferase
topic Starch
Enzymatic modification
Pasting properties
Cyclodextrin
Glycosyltransferase
description Cyclodextrin glycosyltransferase (CGTase) has been used to produce cyclodextrins (CDs) from starches, but their ability to modify starches has been barely explored. The effect of CGTase on corn starch at sub-gelatinization temperature (50 °C) and at different pH conditions, pH 4.0 and pH 6.0, was evaluated. Biochemical features, thermal and structural analysis, oligosaccharides and CDs content were studied. Microscopic analysis of the granules confirmed the enzymatic modification of the starches obtaining structures with irregular surface and small pinholes. The extent of the starch modification was largely dependent on the pHs, being higher at pH 6.0. This was also confirmed by the low viscosity of the resulting pastes during a heating and cooling cycle. Thermal parameters were not affected due to enzymatic treatment. Modified starches were less susceptible to undergo α-amylase hydrolysis. CDs released were higher for samples treated at pH 4.0. Therefore, CGTase modification of corn starches at sub-gelatinization temperature offers an attractive alternative for obtaining porous starches with different properties depending on the pH conditions.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/132245
url http://hdl.handle.net/10261/132245
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.ijbiomac.2016.03.012
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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