A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation

Spermcompetition, a prevalent evolutionary process inwhich the spermatozoa of two or more males compete for the fertilization of the same ovum, leads to morphological and physiological adaptations, including increases in energetic metabolism that may serve to propel sperm faster but that may have ne...

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Detalhes bibliográficos
Autores: Delbarco-Trillo, Javier, García-Álvarez, Olga, Soler, Ana J., Tourmente, Maximiliano, Garde, José Julián, Roldán, Eduardo R. S.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/145719
Acesso em linha:http://hdl.handle.net/10261/145719
Access Level:acceso abierto
Palavra-chave:Sperm competition
Sperm chromatin structure assay
Oxidative stress
Capacitation
Sperm DNA fragmentation
Rodents
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spelling A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentationDelbarco-Trillo, JavierGarcía-Álvarez, OlgaSoler, Ana J.Tourmente, MaximilianoGarde, José JuliánRoldán, Eduardo R. S.Sperm competitionSperm chromatin structure assayOxidative stressCapacitationSperm DNA fragmentationRodentsSpermcompetition, a prevalent evolutionary process inwhich the spermatozoa of two or more males compete for the fertilization of the same ovum, leads to morphological and physiological adaptations, including increases in energetic metabolism that may serve to propel sperm faster but that may have negative effects onDNA integrity. SpermDNA damage is associated with reduced rates of fertilization, embryo and fetal loss, offspring mortality, and mutations leading to genetic disease. We tested whether high levels of sperm competition affect sperm DNA integrity. We evaluated sperm DNA integrity in 18 species of rodents that differ in their levels of spermcompetition using the spermchromatin structure assay. DNA integrity was assessed upon sperm collection, in response to incubation under capacitating or non-capacitating conditions, and after exposure to physical and chemical stressors. Sperm DNA was very resistant to physical and chemical stressors, whereas incubation in noncapacitating and capacitating conditions resulted in only a small increase in spermDNA damage. Importantly, levels of spermcompetition were positively associated with sperm DNA fragmentation across rodent species. This is the first evidence showing that high levels of sperm competition lead to an important cost in the form of increased sperm DNA damage.Peer reviewedPeer ReviewedRoyal Society (Great Britain)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/145719reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDelbarco-Trillo, Javier; García-Álvarez, Olga; Soler, Ana J.; Tourmente, Maximiliano; Garde, José Julián; Roldán, Eduardo R. S. (2016): Data from: A cost for high levels of sperm competition in rodents: increased sperm DNA fragmentation [Dataset]; Dryad; Version 1; https://doi.org/10.5061/dryad.cf2q1Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1457192026-05-22T06:33:51Z
dc.title.none.fl_str_mv A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
title A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
spellingShingle A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
Delbarco-Trillo, Javier
Sperm competition
Sperm chromatin structure assay
Oxidative stress
Capacitation
Sperm DNA fragmentation
Rodents
title_short A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
title_full A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
title_fullStr A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
title_full_unstemmed A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
title_sort A cost for high levels of sperm competition in rodents: Increased sperm DNA fragmentation
dc.creator.none.fl_str_mv Delbarco-Trillo, Javier
García-Álvarez, Olga
Soler, Ana J.
Tourmente, Maximiliano
Garde, José Julián
Roldán, Eduardo R. S.
author Delbarco-Trillo, Javier
author_facet Delbarco-Trillo, Javier
García-Álvarez, Olga
Soler, Ana J.
Tourmente, Maximiliano
Garde, José Julián
Roldán, Eduardo R. S.
author_role author
author2 García-Álvarez, Olga
Soler, Ana J.
Tourmente, Maximiliano
Garde, José Julián
Roldán, Eduardo R. S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sperm competition
Sperm chromatin structure assay
Oxidative stress
Capacitation
Sperm DNA fragmentation
Rodents
topic Sperm competition
Sperm chromatin structure assay
Oxidative stress
Capacitation
Sperm DNA fragmentation
Rodents
description Spermcompetition, a prevalent evolutionary process inwhich the spermatozoa of two or more males compete for the fertilization of the same ovum, leads to morphological and physiological adaptations, including increases in energetic metabolism that may serve to propel sperm faster but that may have negative effects onDNA integrity. SpermDNA damage is associated with reduced rates of fertilization, embryo and fetal loss, offspring mortality, and mutations leading to genetic disease. We tested whether high levels of sperm competition affect sperm DNA integrity. We evaluated sperm DNA integrity in 18 species of rodents that differ in their levels of spermcompetition using the spermchromatin structure assay. DNA integrity was assessed upon sperm collection, in response to incubation under capacitating or non-capacitating conditions, and after exposure to physical and chemical stressors. Sperm DNA was very resistant to physical and chemical stressors, whereas incubation in noncapacitating and capacitating conditions resulted in only a small increase in spermDNA damage. Importantly, levels of spermcompetition were positively associated with sperm DNA fragmentation across rodent species. This is the first evidence showing that high levels of sperm competition lead to an important cost in the form of increased sperm DNA damage.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/145719
url http://hdl.handle.net/10261/145719
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Delbarco-Trillo, Javier; García-Álvarez, Olga; Soler, Ana J.; Tourmente, Maximiliano; Garde, José Julián; Roldán, Eduardo R. S. (2016): Data from: A cost for high levels of sperm competition in rodents: increased sperm DNA fragmentation [Dataset]; Dryad; Version 1; https://doi.org/10.5061/dryad.cf2q1
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society (Great Britain)
publisher.none.fl_str_mv Royal Society (Great Britain)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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